LOCUS       pBbA2c-LasI             3218 bp ds-DNA     circular     15-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     347..366
                     /label="CAT-R"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(433..535)
                     /label="cat promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(565..1188)
                     /label="TetR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     1096..1115
                     /label="Tet-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1207..1262
                     /label="tetR/tetA promoters"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1243..1261
                     /label="tet operator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1272..1299)
                     /label="pBbA2c-RFP REV"
                     /note="sequence: cgaccgatctgaacgatcaTatgtatatctccttcttaaaagatcttt"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1300..1319
                     /label="LasI FWD"
                     /note="sequence: tttaagaaggagatatacatAtgatcgttcagatcggtcg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1300..1908
                     /label="LasI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1888..1908)
                     /label="LasI REV"
                     /note="sequence: ccttactcgagtttggatccttattaggaaacagccagacg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1909..1928
                     /label="pBbA2c-RFP FWD"
                     /note="sequence: gtctggctgtttcctaataaggatccaaactcgagtaagg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      1941..2012
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      2028..2055
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2083..2100)
                     /label="L4440"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(2217..2762)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(2297..2316)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      2876..2970
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2991..432)
                     /label="CmR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
       61 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      121 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      181 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      241 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      301 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      361 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      421 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      481 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
      541 tcattttcgc cagatatcga cgtcttaaga cccactttca catttaagtt gtttttctaa
      601 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
      661 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
      721 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
      781 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
      841 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
      901 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
      961 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat
     1021 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc
     1081 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag
     1141 cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta
     1201 atttttgttg acactctatc gttgatagag ttattttacc actccctatc agtgatagag
     1261 aaaagaattc aaaagatctt ttaagaagga gatatacatA tgatcgttca gatcggtcgt
     1321 cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc
     1381 aaagaacgta aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac
     1441 gctctgtccc cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt
     1501 tgctggcgta tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg
     1561 ctgcacggta aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc
     1621 aactccggtc agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct
     1681 ctggctcgtt actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt
     1741 gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa
     1801 atcggtatcg aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct
     1861 ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt cctaataagg atccaaactc
     1921 gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1981 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2041 gtgggccttt ctgcgtttat acctagggat atattccgct tcctcgctca ctgactcgct
     2101 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     2161 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     2221 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     2281 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     2341 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     2401 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     2461 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2521 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     2581 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     2641 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     2701 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     2761 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     2821 caaatgtagc acctgaagtc agccccatac gatataagtt gttactagtg cttggattct
     2881 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2941 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     3001 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     3061 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     3121 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     3181 tcaaaactgg tgaaactcac ccagggattg gctgagac
//