LOCUS       Exported                7558 bp DNA     circular SYN 15-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB200128-1150amg
VERSION     .
KEYWORDS    AAV-GFAP(long)-FLEX-GCaMP7f
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
COMMENT     Alias: VB200128-1150amg
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
     promoter        169..2346
                     /label=GFAP_long
                     /note="/application_notes=Tissue specificity: Brain. Cell 
                     typespecificity: Astrocytes."
                     /note="/description=Human glial fibrillary acidic 
                     proteinpromoter (2.1 kb)"
                     /note="/full_name=Human glial fibrillary acidic protein 
                     promoter"
                     /note="/official_designation=GFAP"
                     /note="/uuid=cfeed5fca4044be9b6b6531b0560debb"
     misc_feature    2371..2404
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutionsof LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=a19dd1e203df40eb846e5102df13ffb4"
     misc_feature    2437..2470
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=b48e25a55648467f8c98b3f68faf613f"
     misc_feature    complement(2483..3835)
                     /label={GCaMP7f}
                     /label=nonstandard type:  ORF
                     /note="/application_notes="
                     /note="/official_designation={GCaMP7f}"
                     /note="/uuid=194006c259c34a6a85511fa786435ace"
     misc_feature    complement(3836..3841)
                     /label=Kozak
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=ba4f837cfff042e38e7561244b24cfa6"
     misc_feature    complement(3848..3881)
                     /label=Lox2272
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase;can't work with other types of Lox site."
                     /note="/description=Mutated Lox site with two base 
                     substitutionsof LoxP"
                     /note="/official_designation=Lox2272"
                     /note="/uuid=991e0551b2034abd9c3ad4e364260ba6"
     misc_feature    complement(3914..3947)
                     /label=LoxP
                     /label=nonstandard type:  Miscellaneous
                     /note="/application_notes=Recognition site of Cre 
                     recombinase."
                     /note="/description=Locus of X(cross)-over in P1"
                     /note="/official_designation=LoxP"
                     /note="/uuid=f4dc79ba31354e87bba6736ab88dbb23"
     misc_feature    3978..4575
                     /label=WPRE
                     /label=nonstandard type:  Regulatory_Elem
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     polyA_signal    4606..4813
                     /label=BGH pA
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     misc_feature    complement(4821..4961)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/application_notes=Allows rescue of virus from 
                     recombinantplasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
     misc_feature    5878..6738
                     /label=AmpiciIIin
                     /label=nonstandard type:  ORF
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
     rep_origin      6909..7497
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgga gctcccacct
      181 ccctctctgt gctgggactc acagagggag acctcaggag gcagtctgtc catcacatgt
      241 ccaaatgcag agcataccct gggctgggcg cagtggcgca caactgtaat tccagcactt
      301 tgggaggctg atgtggaagg atcacttgag cccagaagtt ctagaccagc ctgggcaaca
      361 tggcaagacc ctatctctac aaaaaaagtt aaaaaatcag ccacgtgtgg tgacacacac
      421 ctgtagtccc agctattcag gaggctgagg tgaggggatc acttaaggct gggaggttga
      481 ggctgcagtg agtcgtggtt gcgccactgc actccagcct gggcaacagt gagaccctgt
      541 ctcaaaagac aaaaaaaaaa aaaaaaaaaa aagaacatat cctggtgtgg agtaggggac
      601 gctgctctga cagaggctcg ggggcctgag ctggctctgt gagctgggga ggaggcagac
      661 agccaggcct tgtctgcaag cagacctggc agcattgggc tggccgcccc ccagggcctc
      721 ctcttcatgc ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag
      781 tgcctgcttc ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca
      841 gggggcttgc attgcacccc agcctgacag cctggcatct tgggataaaa gcagcacagc
      901 cccctagggg ctgcccttgc tgtgtggcgc caccggcggt ggagaacaag gctctattca
      961 gcctgtgccc aggaaagggg atcaggggat gcccaggcat ggacagtggg tggcaggggg
     1021 ggagaggagg gctgtctgct tcccagaagt ccaaggacac aaatgggtga ggggactggg
     1081 cagggttctg accctgtggg accagagtgg agggcgtaga tggacctgaa gtctccaggg
     1141 acaacagggc ccaggtctca ggctcctagt tgggcccagt ggctccagcg tttccaaacc
     1201 catccatccc cagaggttct tcccatctct ccaggctgat gtgtgggaac tcgaggaaat
     1261 aaatctccag tgggagacgg aggggtggcc agggaaacgg ggcgctgcag gaataaagac
     1321 gagccagcac agccagctca tgcgtaacgg ctttgtggag ctgtcaaggc ctggtctctg
     1381 ggagagaggc acagggaggc cagacaagga aggggtgacc tggagggaca gatccagggg
     1441 ctaaagtcct gataaggcaa gagagtgccg gccccctctt gccctatcag gacctccact
     1501 gccacataga ggccatgatt gacccttaga caaagggctg gtgtccaatc ccagccccca
     1561 gccccagaac tccagggaat gaatgggcag agagcaggaa tgtgggacat ctgtgttcaa
     1621 gggaaggact ccaggagtct gctgggaatg aggcctagta ggaaatgagg tggcccttga
     1681 gggtacagaa caggttcatt cttcgccaaa ttcccagcac cttgcaggca cttacagctg
     1741 agtgagataa tgcctgggtt atgaaatcaa aaagttggaa agcaggtcag aggtcatctg
     1801 gtacagccct tccttccctt tttttttttt tttttttttg tgagacaagg tctctctctg
     1861 ttgcccaggc tggagtggcg caaacacagc tcactgcagc ctcaacctac tgggctcaag
     1921 caatcctcca gcctcagcct cccaaagtgc tgggattaca agcatgagcc accccactca
     1981 gccctttcct tcctttttaa ttgatgcata ataattgtaa gtattcatca tggtccaacc
     2041 aaccctttct tgacccacct tcctagagag agggtcctct tgcttcagcg gtcagggccc
     2101 cagacccatg gtctggctcc aggtaccacc tgcctcatgc aggagttggc gtgcccagga
     2161 agctctgcct ctgggcacag tgacctcagt ggggtgaggg gagctctccc catagctggg
     2221 ctgcggccca accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgggc
     2281 atcgccagtc tagcccactc cttcataaag ccctcgcatc ccaggagcga gcagagccag
     2341 agcaggcaag tttgtacaaa aaagcaggct ataacttcgt ataggatact ttatacgaag
     2401 ttatcctcga gtaccggtca cgcgtacgca agcttgataa cttcgtatag catacattat
     2461 acgaagttat cctagggcca ccttacttcg ctgtcatcat ttgtacaaac tcttcgtagt
     2521 ttacctgacc atccccatcg atgtctgctt ccctgatcat ttcatcaacc tcttcatctg
     2581 ttaacttctc tccaaggttt gtcatcacgt ggcgaagctc tgctgcactg atgtagccat
     2641 tgccatcctt atcaaacaca cggaacgctt ctctaatttc ttcttcactg tctgtgtatt
     2701 tcatttttct tgccatcatt gtcaggaact cagggaagtc gattgtgccg tcaccgtcgg
     2761 catctacttc attgatcatg tcctgcagct ctgcttctgt ggggttctgc cccagagacc
     2821 gcatcaccgt ccccagctcc ttggttgtta ttgtcccatc cccgtccttg tcaaataggg
     2881 agaacagctc tttaaattct gcgatctgct cttcagtcag ttggtccggc aggttgtact
     2941 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgccctta agctcgatgc
     3001 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3061 ccttgaagaa gatggtgcgc tcctggatgt agccttcggg catggcggac ttgaagaagt
     3121 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     3181 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     3241 gcttgccgta ggtggcatcg ccctcaccct cgccggacac gctgaacttg tggccgttta
     3301 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     3361 tcaccatgct ccctccggta ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     3421 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttcgaaa
     3481 gtttggactg cacgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     3541 gggtgttctg ctggtagtgg taggcgagct gcacgccgcc gtcctcgatg ttgtggcgga
     3601 tcttgaagtt cgccttgatg ccgttcttct gcttgtcggc cttgatatag acgttctcga
     3661 gtgagctcag ccgacctata gctctgactg cgtgacctgt cttattccac ttacgacgtg
     3721 atgagtcgac catggtggcg agatccttat cgtcatcgtc gtacagatcc cgacccattt
     3781 gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa cccatggtgg
     3841 ctctagaata acttcgtata aagtatccta tacgaagtta taggtacctg agctcaacat
     3901 cgatagcact agtataactt cgtataatgt atgctatacg aagttatacc cagctttctt
     3961 gtacaaagtg ggaattccga taatcaacct ctggattaca aaatttgtga aagattgact
     4021 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4081 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4141 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4201 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4261 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4321 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     4381 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4441 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4501 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4561 gcctccccgc atcgggaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt
     4621 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4681 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4741 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     4801 ggcatgctgg ggagggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     4861 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     4921 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     4981 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     5041 ccctgtagcg gcgcattaag cgcggcgggg gtggtggtta cgcgcagcgt gaccgctaca
     5101 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5161 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5221 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     5281 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5341 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     5401 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5461 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     5521 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5581 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5641 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5701 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5761 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5821 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5881 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5941 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6001 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6061 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     6601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
