LOCUS       Exported                6907 bp DNA     circular SYN 16-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB211117-1080cmd
VERSION     .
KEYWORDS    VB211117-1080cmd.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 16, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functionalequivalent of wild-type 5' ITR)"
                     /note="/name=5' ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=5f14ec1a645a407dab2725d71bd1c86a"
                     /note="/vntifkey=21"
     misc_feature    169..174
                     /label=BglII
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=BglII"
                     /note="/official_designation=BglII"
                     /note="/uuid=00295cad732446d0a5047be780aa021b"
                     /note="/vntifkey=21"
     promoter        175..2204
                     /label={TPH2}
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name={TPH2}"
                     /note="/official_designation={TPH2}"
                     /note="/uuid=efa65a9b00b04004b900cf1f019f8293"
                     /note="/vntifkey=30"
     misc_feature    2205..2210
                     /label=AgeI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows linearization of vector."
                     /note="/full_name=AgeI restriction enzyme site"
                     /note="/name=AgeI"
                     /note="/official_designation=AgeI"
                     /note="/uuid=1a230db670374afd9cc5150e9d8d9944"
                     /note="/vntifkey=21"
     misc_feature    2235..2240
                     /label=Kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
     misc_feature    2241..3296
                     /label=Cre
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Most commonly used 
                     site-specificrecombinase"
                     /note="/full_name=Cyclization recombinase"
                     /note="/name=Cre"
                     /note="/official_designation=Cre"
                     /note="/uuid=1749f2b2045243a587f477d238cc8911"
                     /note="/vntifkey=21"
     misc_feature    3327..3924
                     /label=WPRE
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Enhances virus stability in 
                     packagingcells, leading to higher titer of packaged virus; 
                     enhances higher expression of transgenes."
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
                     /note="/name=WPRE"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
                     /note="/vntifkey=21"
     polyA_signal    3955..4162
                     /label=BGH pA
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows transcription termination 
                     andpolyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/name=BGH pA"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
                     /note="/vntifkey=25"
     misc_feature    complement(4170..4310)
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows replication of the viral 
                     genomeand its packaging into virus."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/name=3' ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /note="/vntifkey=21"
     misc_feature    5227..6087
                     /label=AmpiciIIin
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpiciIIin"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /note="/vntifkey=21"
     rep_origin      6258..6846
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=pUC ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgag atctggactg
      181 accactccgt gcctttgctt ttcgtgtctt ctgcagagag gtcgataccc tttgttctac
      241 ttggttatcg cacaacttga ttctctcagc cagactgtga tctccgtggg tcagtgctgc
      301 atcgaacact tctttgcatg cttgggattc agtttcagct tactagaaac tcagggctca
      361 acaaaatttt gttgagttat acatctgttc tgtgtctggc tctgtagtct gtcgagtctg
      421 cttttcttta cacccttctc cttctttgga agagaacacg gattcccaaa gtcaaattga
      481 ctgaattaag atcctacctt agacattctt ttgtgcctta gtacacacac acacatacac
      541 acacacacac acacacacac acagatgcac acacatacac acatatgcac acacacatac
      601 acacagatgc acacacacac atacacatac acacagatgc acacacgcat acacatacac
      661 acagatgcac acacacatac acatacacac agatgcacac acgcacacac atacacatac
      721 acacatgcac acgcacacat aaacatacac acagatgcac acacgcacac acataaacat
      781 acacatgcac acacacacac acatgcacac acacatgcac atgcacacac acacacacac
      841 accagtttta tgataactga ctatgttctg acctataaac gtgcataaaa atccagaata
      901 tgattactta gcattgttag ctattatttt ttacaattac ttcagttaat ttattaataa
      961 tttctcttcc tcttagcctc ttagtcttca gaggaaatag gagctaaatg attatttcca
     1021 gagttggatc aaataataat cctaaaccct caagtctcca gaaacagtta aaatccaacc
     1081 aatgttaaca tatacagact tgttttgaag aatcttcatt ttgttttcta tacaatccaa
     1141 ttgtacgagt cccagatttt gtttagttct tcagttaaac atgtactctt ggttaagaat
     1201 tttccatgtt taaaagttat ttgttatttt tacgtacatg agtacactgt agctgtcttc
     1261 atacacacca gaagagggca tcaaatcccc attacatatg attgtgagcc acctagtggt
     1321 tgctgggatt tgaactcagg acctctggaa gagcagtcag cactcttaac cactaagcta
     1381 tctctccagc cccaaacttt ccattcttta aaggattgct aagctttccc gtggctttct
     1441 aaagttggga aggtacctat agaattttgt ctatgcctgt cagattgctg ggtctgatta
     1501 agttatagat ggggagcagc agaattgcat cagaaaagca tcaaagggat agtgggccta
     1561 tgggcatttc atttccatat gtttcttact tagtgaagaa cattctagaa ggtgagcatc
     1621 ttggctaagt tcaagcctcg aatttcaata gcctgagtaa ccttctacct ggagacacca
     1681 caggttttgg aaacaggcct cttacgaaag ccatcacaca tacatacaca taaaatttgc
     1741 acatcgtaac atgtcatggt gcagaaaagc cttccttaag ctattttctt acttttagaa
     1801 agcacaaagc actcaaactc atagttactg gaagactgag ggtaaccttc caatagcagg
     1861 ccctagaatt aaaattagac gactgctatt ttgttgcaag gaaaaaaata ataataaacc
     1921 ctgacgcata gctctccacc atctcttccc aaagggctac tcgacctatg aaacaaacaa
     1981 atctcatcat gagcacagat aaccccaggc ttcagacgtg taatctgatt gtggccatca
     2041 gcaaccagaa atgagttttt tctaatcagc ttcccatcac tcctcagtca ctcatataaa
     2101 ggaacacagg gaggggagga agcgcactgc tcttcagcac cagggttctg gacagcgcct
     2161 cgagcagcca gctgccgctc accttcctcc tacatctctg ccgcaccggt caagtttgta
     2221 caaaaaagca ggctgccacc atgggcccaa agaagaagag aaaggtttcg aatttactga
     2281 ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga
     2341 acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc
     2401 tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg
     2461 cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa
     2521 aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac
     2581 gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg
     2641 atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc
     2701 gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga
     2761 ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct
     2821 cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca
     2881 ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt
     2941 ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg
     3001 gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg
     3061 aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct
     3121 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
     3181 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
     3241 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattagaccc
     3301 agctttcttg tacaaagtgg gaattccgat aatcaacctc tggattacaa aatttgtgaa
     3361 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3421 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3481 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3541 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3601 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3661 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3721 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3781 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3841 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3901 ctttgggccg cctccccgca tcgggaattc ctagagctcg ctgatcagcc tcgactgtgc
     3961 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4021 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4081 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4141 agaatagcag gcatgctggg gagggccgca ggaaccccta gtgatggagt tggccactcc
     4201 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4261 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4321 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4381 tagtacgcgc cctgtagcgg cgcattaagc gcggcggggg tggtggttac gcgcagcgtg
     4441 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     4501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4681 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     4741 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     4801 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4861 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4921 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4981 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5941 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6901 cacatgt
//
