LOCUS       3xGGGGS_TRIPalpha_E32G_ 6240 bp ds-DNA     circular     22-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(214..896)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(994..1653)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(2114..2420)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(2439..2507)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2512..2676
                     /label="ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3050..3058
                     /label="kozak2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3059..3097
                     /label="alpha tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3101..4417
                     /label="hHTR6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4448..4456
                     /label="kozak2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4448..4457
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4448..4457
                     /label="New Annotation"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_fwdcolor="#7ea7d8"
     CDS             4454..4999
                     /label="SNAP-tag"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4454..5174
                     /label="Translation 4454-5174"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAVKEWLLAHEGHRLGKPGLGGGGGSGGGGSGGGGSASEGADGAICGYNLATLVMLGPSERVFCPLCGPCSSDIYELM*"
     misc_feature    6074..6240
                     /label="ITR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
       61 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1981 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     2221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     2341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     2401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     2461 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct gcgcgctcgc
     2521 tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc
     2581 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttccttg
     2641 tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag gaagatcggc
     2701 cgcacgcgtc tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
     2761 tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     2821 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     2881 gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     2941 tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
     3001 gctcgtttag tgaaccgtca gatcgcctgg agaattcggt accacgcgtg ccaccatgtc
     3061 acgacttgag gaggaactca gaaggagact cactgaacca gtcccagagc cgggcccaac
     3121 cgccaatagc accccggcct ggggggcagg gccgccgtcg gccccggggg gcagcggctg
     3181 ggtggcggcc gcgctgtgcg tggtcatcgc gctgacggcg gcggccaact cgctgctgat
     3241 cgcgctcatc tgcactcagc ccgcgctgcg caacacgtcc aacttcttcc tggtgtcgct
     3301 cttcacgtct gacctgatgg tggggctggt ggtgatgccg ccggccatgc tgaacgcgct
     3361 gtacgggcgc tgggtgctgg cgcgcggcct ctgcctgctc tggaccgcct tcgacgtgat
     3421 gtgctgcagc gcctccatcc tcaacctctg cctcatcagc ctggaccgct acctgctcat
     3481 cctctcgccg ctgcgctaca agctgcgcat gacgcccctg cgtgccctgg ccctagtcct
     3541 gggcgcctgg agcctcgccg ctctcgcctc cttcctgccc ctgctgctgg gctggcacga
     3601 gctgggccac gcacggccac ccgtccctgg ccagtgccgc ctgctggcca gcctgccttt
     3661 tgtccttgtg gcgtcgggcc tcaccttctt cctgccctcg ggtgccatat gcttcaccta
     3721 ctgcaggatc ctgctagctg cccgcaagca ggccgtgcag gtggcctccc tcaccaccgg
     3781 catggccagt caggcctcgg agacgctgca ggtgcccagg accccacgcc caggggtgga
     3841 gtctgctgac agcaggcgtc tagccacgaa gcacagcagg aaggccctga aggccagcct
     3901 gacgctgggc atcctgctgg gcatgttctt tgtgacctgg ttgcccttct ttgtggccaa
     3961 catagtccag gccgtgtgcg actgcatctc cccaggcctc ttcgatgtcc tcacatggct
     4021 gggttactgt aacagcacca tgaaccccat catctaccca ctcttcatgc gggacttcaa
     4081 gcgggcgctg ggcaggttcc tgccatgtcc acgctgtccc cgggagcgcc aggccagcct
     4141 ggcctcgcca tcactgcgca cctctcacag cggcccccgg cccggcctta gcctacagca
     4201 ggtgctgccg ctgcccctgc cgccggactc agattcggac tcagacgcag gctcaggcgg
     4261 ctcctcgggc ctgcggctca cggcccagct gctgcttcct ggcgaggcca cccaggaccc
     4321 cccgctgccc accagggccg ctgccgccgt caatttcttc aacatcgacc ccgcggagcc
     4381 cgagctgcgg ccgcatccac ttggcatccc cacgaacaac ggtacagcgg gcccgggatc
     4441 cgcgatcgcc accatggaca aagactgcga aatgaagcgc accaccctgg atagccctct
     4501 gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa
     4561 aggaacatct gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc
     4621 agagccactg atgcaggcca ccgcctggct caacgcctac tttcaccagc ctgaggccat
     4681 cgaggagttc cctgtgccag ccctgcacca cccagtgttc cagcaggaga gctttacccg
     4741 ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga gaggtcatca gctacagcca
     4801 cctggccgcc ctggccggca atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg
     4861 aaatcccgtg cccattctga tcccctgcca ccgggtggtg cagggcgacc tggacgtggg
     4921 gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg gccacagact
     4981 gggcaagcct gggctgggtG GAGGAGGAGG ATCCGGAGGA GGAGGATCTG GAGGAGGAGG
     5041 ATCTGCCAGC GAGGGAGCTG ACGGAGCTAT CTGTGGCTAC AACCTGGCCA CCCTGGTCAT
     5101 GCTGGGACCA AGCGAGAGGG TGTTCTGCCC ACTGTGCGGA CCTTGCAGCT CCGATATCTA
     5161 CGAGCTGATG tgaactagta agcttatcga taatcaacct ctggattaca aaatttgtga
     5221 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5281 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5341 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5401 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5461 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5521 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5581 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5641 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5701 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5761 cctttgggcc gcctccccgc atcgataccg tcgacctcga cccgggcggc cgcttcgagc
     5821 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5881 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5941 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     6001 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatcttcc
     6061 tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc
     6121 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     6181 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
//