LOCUS       HisSumoOphP-pPIC       11584 bp DNA     circular SYN 18-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Rutherford Appleton Laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2022 from SnapGene 6.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             950..3541
                     /codon_start=1
                     /label=SumoOphP
                     /translation="MGHHHHHHHHGGSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSE
                     IFFKIKKTTPLRRLMEAFAKRQGKEMDSLTFLYDGIEIQADQTPEDLDMEDNDIIEAHR
                     EQIGGENLYFQGTSMSFPGWGPYPPVERDETSAITYSSKLHGSVTVRDPYSQLEVPFED
                     SEETKAFVHSQRKFARTYLDENPDREAWLETLKKSWNYRRFSALKPESDGHYYFEYNDG
                     LQSQLSLYRVRMGEEDTVLTESGPGGELFFNPNLLSLDGNAALTGFVMSPCGNYWAYGV
                     SEHGSDWMSIYVRKTSSPHLPSQERGKDPGRMNDKIRHVRFFIVSWTSDSKGFFYSRYP
                     PEDDEGKGNAPAMNCMVYYHRIGEDQESDVLVHEDPEHPFWISSVQLTPSGRYILFAAS
                     RDASHTQLVKIADLHENDIGTNMKWKNLHDPWEARFTIVGDEGSKIYFMTNLKAKNYKV
                     ATFDANHPDEGLTTLIAEDPNAFLVSASIHAQDKLLLVYLRNASHEIHIRDLTTGKPLG
                     RIFEDLLGQFMVSGRRQDNDIFVLFSSFLSPGTVYRYTFGEEKGYRSLFRAISIPGLNL
                     DDFMTESVFYPSKDGTSVHMFITRPKDVLLDGTSPVLQYGYGGFSLAMLPTFSLSTLLF
                     CKIYRAIYAIPNIRGGSEYGESWHREGMLDKKQNVFDDFNAATEWLIANKYASKDRIAI
                     RGGSNGGVLTTACANQAPGLYRCVITIEGIIDMLRFPKFTFGASWRSEYGDPEDPEDFD
                     FIFKYSPYHNIPPPGDTVMPAMLFFTAAYDDRVSPLHTFKHVAALQHNFPKGPNPCLMR
                     IDLNSGHFAGKSTQEMLEETADEYSFIGKSMGLTMQTQGSVDSSRWSCVTV"
     CDS             956..979
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1277..1297
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      3629..3875
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(4288..6822)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(7236..8051)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7236..8051)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    8430..9186
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    9329..9469
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9655..10243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10414..11274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11275..11379)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccgaaacta tgggtcatca
      961 tcatcatcat catcatcatg gtggttctga ctccgaggtc aaccaggagg ctaagcctga
     1021 ggttaagcca gaggttaagc ctgagaccca cattaacttg aaggtttccg acggttcatc
     1081 cgaaatcttc ttcaagatta agaagactac cccattgaga agattgatgg aggccttcgc
     1141 caagagacaa ggaaaggaga tggactcctt gactttcttg tacgacggta tcgagatcca
     1201 ggccgaccaa accccagagg acttggacat ggaggacaac gacatcatcg aggctcacag
     1261 agagcaaatt ggtggtgaga acttgtactt ccaaggtact agtatgtcgt ttccaggatg
     1321 gggaccatat ccacctgttg aaagggacga aacttcagcg ataacttaca gtagcaagtt
     1381 gcatggctct gttacggttc gtgatcccta cagtcaactt gaagtacctt tcgaggatag
     1441 tgaagaaacg aaggcgtttg ttcattcaca acgcaagttt gcacgaacct atctagatga
     1501 aaatcccgat cgagaagcat ggctcgagac gctgaagaag agctggaatt acaggagatt
     1561 tagcgcattg aaacctgaaa gcgacggaca ttactacttc gagtataacg atggccttca
     1621 atctcagctt tccctgtatc gtgtgaggat gggggaggag gataccgtcc tcactgagtc
     1681 tggtcctggt ggagaactct ttttcaatcc caacttgctt tcccttgatg gcaacgccgc
     1741 acttactggt ttcgtcatga gtccatgtgg gaattattgg gcatatggcg tatctgaaca
     1801 tggatccgac tggatgtcta tttacgttcg aaagacgtcg tctccgcatc ttccgtcaca
     1861 agaacgagga aaggatcctg gacggatgaa cgacaagatt cgacacgtcc gtttcttcat
     1921 tgtatcttgg acaagtgaca gcaaaggttt tttctattct aggtatcctc ctgaggatga
     1981 cgaagggaaa ggtaatgcac cagcaatgaa ttgcatggtc tattatcatc ggattggcga
     2041 ggatcaggag agtgacgtcc ttgttcatga ggatcctgag catcctttct ggatctcgtc
     2101 ggtgcagctc actccgagcg gccgatacat cctctttgcc gcgagccgcg acgcaagtca
     2161 cactcaatta gtcaagatcg cagatcttca cgaaaatgac attgggacga atatgaaatg
     2221 gaaaaacctt catgatccat gggaggctag gtttacaata gtcggagacg agggttcaaa
     2281 gatctatttc atgaccaatc tcaaggccaa gaactacaaa gtggcgacgt ttgatgccaa
     2341 ccacccagac gagggtctta cgacgctcat agcagaagac cctaacgcgt ttctcgtttc
     2401 agcaagcatt cacgcacaag acaagctcct acttgtttat cttcgtaacg ccagccacga
     2461 gattcatatc cgagatctaa ccactgggaa gcccctcggg cgtatctttg aggatttact
     2521 tggacagttt atggtatcag gtcgacggca ggataacgat attttcgttc tctttagcag
     2581 tttcctctct cctggaactg tgtatcggta cacgttcggt gaagagaaag gataccgttc
     2641 actatttcgt gctataagta ttccgggcct caatctagat gactttatga cagagtcggt
     2701 tttctatcca tccaaggacg ggacttctgt tcacatgttt atcacccgcc cgaaggatgt
     2761 actgctcgat ggaacttccc ctgtcctaca atacggctat ggcggtttct ccctagcaat
     2821 gcttcctaca ttctccctct ctacgctgct attctgcaaa atctacaggg caatctacgc
     2881 gatacccaat attcgtggtg gttcggaata cggcgagtca tggcaccggg agggtatgtt
     2941 agacaagaag cagaacgtgt ttgacgactt caacgctgca accgaatggc ttattgcgaa
     3001 taagtacgca agcaaggatc gcattgccat ccgaggagga tcaaatggag gtgttttgac
     3061 cactgcatgt gcaaatcaag caccgggact ctaccgttgt gtgatcacaa ttgagggtat
     3121 aattgatatg cttagatttc ctaagtttac atttggcgct agctggcgtt ctgaatatgg
     3181 cgatcctgag gatcctgaag atttcgactt catattcaaa tactccccct atcacaacat
     3241 ccctcccccg ggagacactg tcatgccagc catgctattc tttactgcgg cgtatgatga
     3301 tcgtgtctcg cctttgcaca cgttcaagca cgttgcggca ttgcaacaca actttcccaa
     3361 aggcccgaat ccatgcctaa tgcgcatcga cttgaattcg ggacactttg ctggcaagag
     3421 tacgcaggag atgttggaag aaacggcaga tgaatacagc tttatcggga agtctatggg
     3481 cctcactatg cagactcaag gttcagtgga ttcaagccgc tggtcctgcg taacagttta
     3541 agcggccgcg aattaattcg ccttagacat gactgttcct cagttcaagt tgggcactta
     3601 cgagaagacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca tttgcctgag
     3661 agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat aggatttttt
     3721 ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc gcagctgatg
     3781 aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc ttggtatttc
     3841 ccactcctct tcagagtaca gaagattaag tgagaagttc gtttgtgcaa gcttatcgat
     3901 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     3961 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
     4021 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
     4081 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
     4141 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
     4201 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat ctatcgaatc
     4261 taaatgtaag ttaaaatctc taaataatta aataagtccc agtttctcca tacgaacctt
     4321 aacagcattg cggtgagcat ctagaccttc aacagcagcc agatccatca ctgcttggcc
     4381 aatatgtttc agtccctcag gagttacgtc ttgtgaagtg atgaacttct ggaaggttgc
     4441 agtgttaact ccgctgtatt gacgggcata tccgtacgtt ggcaaagtgt ggttggtacc
     4501 ggaggagtaa tctccacaac tctctggaga gtaggcacca acaaacacag atccagcgtg
     4561 ttgtacttga tcaacataag aagaagcatt ctcgatttgc aggatcaagt gttcaggagc
     4621 gtactgattg gacatttcca aagcctgctc gtaggttgca accgataggg ttgtagagtg
     4681 tgcaatacac ttgcgtacaa tttcaaccct tggcaactgc acagcttggt tgtgaacagc
     4741 atcttcaatt ctggcaagct ccttgtctgt catatcgaca gccaacagaa tcacctggga
     4801 atcaatacca tgttcagctt gagacagaag gtctgaggca acgaaatctg gatcagcgta
     4861 tttatcagca ataactagaa cttcagaagg cccagcaggc atgtcaatac tacacagggc
     4921 tgatgtgtca ttttgaacca tcatcttggc agcagtaacg aactggtttc ctggaccaaa
     4981 tattttgtca cacttaggaa cagtttctgt tccgtaagcc atagcagcta ctgcctgggc
     5041 gcctcctgct agcacgatac acttagcacc aaccttgtgg gcaacgtaga tgacttctgg
     5101 ggtaagggta ccatccttct taggtggaga tgcaaaaaca atttctttgc aaccagcaac
     5161 tttggcagga acacccagca tcagggaagt ggaaggcaga attgcggttc caccaggaat
     5221 atagaggcca actttctcaa taggtcttgc aaaacgagag cagactacac cagggcaagt
     5281 ctcaacttgc aacgtctccg ttagttgagc ttcatggaat ttcctgacgt tatctataga
     5341 gagatcaatg gctctcttaa cgttatctgg caattgcata agttcctctg ggaaaggagc
     5401 ttctaacaca ggtgtcttca aagcgactcc atcaaacttg gcagttagtt ctaaaagggc
     5461 tttgtcacca ttttgacgaa cattgtcgac aattggtttg actaattcca taatctgttc
     5521 cgttttctgg ataggacgac gaagggcatc ttcaatttct tgtgaggagg ccttagaaac
     5581 gtcaattttg cacaattcaa tacgaccttc agaagggact tctttaggtt tggattcttc
     5641 tttaggttgt tccttggtgt atcctggctt ggcatctcct ttccttctag tgacctttag
     5701 ggacttcata tccaggtttc tctccacctc gtccaacgtc acaccgtact tggcacatct
     5761 aactaatgca aaataaaata agtcagcaca ttcccaggct atatcttcct tggatttagc
     5821 ttctgcaagt tcatcagctt cctccctaat tttagcgttc aacaaaactt cgtcgtcaaa
     5881 taaccgtttg gtataagaac cttctggagc attgctctta cgatcccaca aggtggcttc
     5941 catggctcta agaccctttg attggccaaa acaggaagtg cgttccaagt gacagaaacc
     6001 aacacctgtt tgttcaacca caaatttcaa gcagtctcca tcacaatcca attcgatacc
     6061 cagcaacttt tgagttgctc cagatgtagc acctttatac cacaaaccgt gacgacgaga
     6121 ttggtagact ccagtttgtg tccttatagc ctccggaata gactttttgg acgagtacac
     6181 caggcccaac gagtaattag aagagtcagc caccaaagta gtgaatagac catcggggcg
     6241 gtcagtagtc aaagacgcca acaaaatttc actgacaggg aactttttga catcttcaga
     6301 aagttcgtat tcagtagtca attgccgagc atcaataatg gggattatac cagaagcaac
     6361 agtggaagtc acatctacca actttgcggt ctcagaaaaa gcataaacag ttctactacc
     6421 gccattagtg aaacttttca aatcgcccag tggagaagaa aaaggcacag cgatactagc
     6481 attagcgggc aaggatgcaa ctttatcaac cagggtccta tagataaccc tagcgcctgg
     6541 gatcatcctt tggacaactc tttctgccaa atctaggtcc aaaatcactt cattgatacc
     6601 attattgtac aacttgagca agttgtcgat cagctcctca aattggtcct ctgtaacgga
     6661 tgactcaact tgcacattaa cttgaagctc agtcgattga gtgaacttga tcaggttgtg
     6721 cagctggtca gcagcatagg gaaacacggc ttttcctacc aaactcaagg aattatcaaa
     6781 ctctgcaaca cttgcgtatg caggtagcaa gggaaatgtc atacttgaag tcggacagtg
     6841 agtgtagtct tgagaaattc tgaagccgta tttttattat cagtgagtca gtcatcagga
     6901 gatcctctac gccggacgca tcgtggccga cctgcagggg gggggggggc gctgaggtct
     6961 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca
     7021 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
     7081 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
     7141 caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg
     7201 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     7261 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     7321 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     7381 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     7441 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     7501 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     7561 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     7621 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     7681 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     7741 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     7801 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     7861 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     7921 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     7981 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
     8041 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     8101 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     8161 tccccccccc ccctgcaggt cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     8221 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     8281 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     8341 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     8401 ctaatgcagg agtcgcataa gggagagcgt cgagtatcta tgattggaag tatgggaatg
     8461 gtgatacccg cattcttcag tgtcttgagg tctcctatca gattatgccc aactaaagca
     8521 accggaggag gagatttcat ggtaaatttc tctgactttt ggtcatcagt agactcgaac
     8581 tgtgagacta tctcggttat gacagcagaa atgtccttct tggagacagt aaatgaagtc
     8641 ccaccaataa agaaatcctt gttatcagga acaaacttct tgtttcgaac tttttcggtg
     8701 ccttgaacta taaaatgtag agtggatatg tcgggtagga atggagcggg caaatgctta
     8761 ccttctggac cttcaagagg tatgtagggt ttgtagatac tgatgccaac ttcagtgaca
     8821 acgttgctat ttcgttcaaa ccattccgaa tccagagaaa tcaaagttgt ttgtctacta
     8881 ttgatccaag ccagtgcggt cttgaaactg acaatagtgt gctcgtgttt tgaggtcatc
     8941 tttgtatgaa taaatctagt ctttgatcta aataatcttg acgagccaag gcgataaata
     9001 cccaaatcta aaactctttt aaaacgttaa aaggacaagt atgtctgcct gtattaaacc
     9061 ccaaatcagc tcgtagtctg atcctcatca acttgagggg cactatcttg ttttagagaa
     9121 atttgcggag atgcgatatc gagaaaaagg tacgctgatt ttaaacgtga aatttatctc
     9181 aagatctctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9241 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9301 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     9361 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     9421 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     9481 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9541 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9601 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9661 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9721 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9781 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9841 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9901 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9961 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10021 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10081 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10141 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10201 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10261 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10321 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10381 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10441 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10501 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10561 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10621 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10681 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
    10741 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10801 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10861 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10921 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10981 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
    11041 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11101 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11161 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11221 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11281 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11341 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11401 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11461 gaggcccttt cgtcttcaag aattaattct catgtttgac agcttatcat cgataagctg
    11521 actcatgttg gtattgtgaa atagacgcag atcgggaaca ctgaaaaata acagttatta
    11581 ttcg
//
