LOCUS       Exported               10433 bp ds-DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      924..1512
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1695..1787
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    1800..1821
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1836..1866
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1874..1890
                     /label=rlac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1935..1953
                     /label=rT3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1981..2207
                     /label=rRSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             2208..2388
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    2435..2560
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2544..2907
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
     misc_feature    3053..3286
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             3663..3763
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    3782..3900
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.8"
                     /note="/other=misc_feature"
     misc_feature    complement(3920..3943)
                     /label=rMCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.8"
                     /note="/match_length=22.2"
                     /note="/other=misc_feature"
     promoter        3946..4456
                     /label=rhPGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4511..4535
                     /label=rattB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     regulatory      4547..4556
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4553..5269
                     /label=rEGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    5270..5320
                     /label=rMCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=77.3"
                     /note="/other=misc_feature"
     CDS             5321..6823
                     /label=rALDH1A1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     protein_bind    complement(6838..6862)
                     /label=rattB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    6893..7481
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(7364..7375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7524..8023
                     /label=rPGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             8037..8840
                     /label=rNeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     CDS             8849..8929
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=81.5"
                     /note="/match_length=27.8"
                     /note="/other=CDS"
     LTR             8932..9165
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     LTR             8985..9165
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    9237..9358
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      9398..9533
                     /label=rSV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(9554..9572)
                     /label=rT7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9580..9735
                     /label=rlacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     primer_bind     complement(9582..9598)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9740..10195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      9740..10168
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        10221..10325
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(10326..10433,1..753)
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     3781 cttttaaaag aaaagggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3841 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3901 atcgatcacg agactagcct cgagaagctt gatatcgaat tccacggggt tggggttgcg
     3961 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     4021 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     4081 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     4141 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     4201 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     4261 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     4321 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     4381 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     4441 cgacctctct ccccaggggg atccaccggt tcgtcgacta gtccagtgtg gtggaattct
     4501 gcagatatca acaagtttgt acaaaaaagc aggctcgcta ccggtcgcca ccatggtgag
     4561 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4621 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4681 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4741 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     4801 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4861 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4921 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4981 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5041 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5101 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5161 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5221 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag
     5281 atctcgagct caagcttcga attctgcagt cgacggtacc atgtcatcct caggcacgcc
     5341 agacttacct gtcctactca ccgatttgaa gattcaatat actaagatct tcataaacaa
     5401 tgaatggcat gattcagtga gtggcaagaa atttcctgtc tttaatcctg caactgagga
     5461 ggagctctgc caggtagaag aaggagataa ggaggatgtt gacaaggcag tgaaggccgc
     5521 aagacaggct tttcagattg gatccccgtg gcgtactatg gatgcttccg agagggggcg
     5581 actattatac aagttggctg atttaatcga aagagatcgt ctgctgctgg cgacaatgga
     5641 gtcaatgaat ggtggaaaac tctattccaa tgcatatctg aatgatttag caggctgcat
     5701 caaaacattg cgctactgtg caggttgggc tgacaagatc cagggccgta caataccaat
     5761 tgatggaaat ttttttacat atacaagaca tgaacctatt ggtgtatgtg gccaaatcat
     5821 tccttggaat ttcccgttgg ttatgctcat ttggaagata gggcctgcac tgagctgtgg
     5881 aaacacagtg gttgtcaaac cagcagagca aactcctctc actgctctcc acgtggcatc
     5941 tttaataaaa gaggcagggt ttcctcctgg agtagtgaat attgttcctg gttatgggcc
     6001 tacagcaggg gcagccattt cttctcacat ggatatagac aaagtagcct tcacaggatc
     6061 aacagaggtt ggcaagttga tcaaagaagc tgccgggaaa agcaatctga agagggtgac
     6121 cctggagctt ggaggaaaga gcccttgcat tgtgttagct gatgccgact tggacaatgc
     6181 tgttgaattt gcacaccatg gggtattcta ccaccagggc cagtgttgta tagccgcatc
     6241 caggattttt gtggaagaat caatttatga tgagtttgtt cgaaggagtg ttgagcgggc
     6301 taagaagtat atccttggaa atcctctgac cccaggagtc actcaaggcc ctcagattga
     6361 caaggaacaa tatgataaaa tactcgacct cattgagagt gggaagaaag aaggggccaa
     6421 actggaatgt ggaggaggcc cgtgggggaa taaaggctac tttgtccagc ccacagtgtt
     6481 ctctaatgtt acagatgaga tgcgcattgc caaagaggag atttttggac cagtgcagca
     6541 aatcatgaag tttaaatctt tagatgacgt gatcaaaaga gcaaacaata ctttctatgg
     6601 cttatcagca ggagtgttta ccaaagacat tgataaagcc ataacaatct cctctgctct
     6661 gcaggcagga acagtgtggg tgaattgcta tggcgtggta agtgcccagt gcccctttgg
     6721 tggattcaag atgtctggaa atggaagaga actgggagag tacggtttcc atgaatatac
     6781 agaggtcaaa acagtcacag tgaaaatctc tcagaagaac tcatctagat aactgatacc
     6841 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc
     6901 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6961 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7021 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7081 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7141 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7201 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7261 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7321 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7381 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7441 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc tgcagatatc
     7501 cggactagtg atctaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     7561 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     7621 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     7681 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     7741 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     7801 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     7861 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     7921 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     7981 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg
     8041 gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     8101 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     8161 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     8221 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     8281 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     8341 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     8401 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     8461 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     8521 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     8581 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     8641 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8701 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     8761 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     8821 ttcttgacga gttcttctga ggggatccgt cgagggcccg gtacctttaa gaccaatgac
     8881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggga ctggaagggc
     8941 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9181 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9361 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9421 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9481 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9541 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9601 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9661 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9721 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9781 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9841 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9901 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9961 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10021 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10081 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10141 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10201 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10321 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10381 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gat
//
