LOCUS       Exported               13014 bp ds-DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      928..1516
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1699..1791
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    1804..1825
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1840..1870
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1874..1896
                     /label=rlac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1902..1918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(1967..2088)
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(2083..2208)
                     /label=rble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
     CDS             complement(2218..2592)
                     /label=rBleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(2611..2658)
                     /label=rEM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        complement(2706..3035)
                     /label=rSV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      2720..2855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(3049..3477)
                     /direction=LEFT
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(3498..3519)
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     polyA_signal    complement(3523..3747)
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     LTR             complement(3779..4012)
                     /label=r3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     CDS             complement(4014..4088)
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
     misc_feature    complement(4084..4672)
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4190..4201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4688..5285)
                     /label=rPuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=99.8"
                     /note="/other=CDS"
     CDS             complement(4688..5284)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             complement(5285..5341)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5351..5374)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5375..5422)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5423..9526)
                     /label=rCas9
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     regulatory      9523..9532
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(9551..9762)
                     /label=rEF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(9788..9806)
                     /label=rsmall t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
     misc_RNA        complement(9824..9899)
                     /label=rgRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     misc_feature    9900..9919
                     /label=ALDH1A3 gRNA1 target
     promoter        complement(9929..10169)
                     /label=rU6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(10220..10338)
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.2"
                     /note="/match_length=100.8"
                     /note="/other=misc_feature"
     CDS             complement(10388..10488)
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    complement(10865..11098)
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(11244..11607)
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
     misc_feature    complement(11591..11716)
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     LTR             complement(11763..11943)
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     promoter        complement(11961..12160)
                     /label=rCMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
     promoter        complement(11961..12159)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        complement(12161..12540)
                     /label=rCMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     misc_feature    12695..12747
                     /label=rbom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
     promoter        12806..12910
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(12911..13014,1..757)
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     1981 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     2101 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     2161 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca
     2221 gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc
     2281 ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt
     2341 cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca
     2401 ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc
     2461 gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc
     2521 ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt
     2581 caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga tatactatgc
     2641 cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca agctcccggg
     2701 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2761 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2821 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2881 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     2941 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     3001 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttcgcgttaa
     3061 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3121 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3181 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3241 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3301 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3361 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3421 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg
     3481 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     3541 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     3601 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     3661 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     3721 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctg
     3781 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     3841 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     3901 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     3961 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     4021 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagtc
     4081 gacgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     4141 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     4201 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     4261 aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     4321 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     4381 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     4441 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     4501 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     4561 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     4621 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgactt
     4681 aacgcgttca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     4741 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     4801 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     4861 cggcgctgcc cagacccttg ccctggtggt cgggcgagac tccgacggtg gccaggaacc
     4921 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     4981 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     5041 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     5101 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     5161 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     5221 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     5281 cggtcggtcc aggattctct tcgacatctc cggcttgttt cagcagagag aagtttgttg
     5341 cgccggatcc cttatcgtca tcgtctttgt aatctttctt cttcttagcc tgtccagcct
     5401 tctttgtggc ggcaggtcgc ttgtcgcctc ccagctgaga caggtcgatc cgtgtctcgt
     5461 acaggccggt gatgctctgg tggatcaggg tggcgtccag cacctctttg gtgctggtgt
     5521 acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc ggcaggggct cccagattgg
     5581 tcagggtaaa caggtggatg atattctcgg cctgctctct gatgggctta tcccggtgct
     5641 tgttgtaggc ggacagcact ttgtccagat tagcgtcggc caggatcact ctcttggaga
     5701 actcgctgat ctgctcgatg atctcgtcca ggtagtgctt gtgctgttcc acaaacagct
     5761 gtttctgctc attatcctcg ggggagccct tcagcttctc atagtggctg gccaggtaca
     5821 ggaagttcac atatttggag ggcagggcca gttcgtttcc cttctgcagt tcgccggcag
     5881 aggccagcat tctcttccgg ccgttttcca gctcgaacag ggagtactta ggcagcttga
     5941 tgatcaggtc ctttttcact tctttgtagc ccttggcttc cagaaagtcg atgggattct
     6001 tctcgaagct gcttctttcc atgatggtga tccccagcag ctctttcaca ctcttcagtt
     6061 tcttggactt gcccttttcc actttggcca ccaccagcac agaataggcc acggtggggc
     6121 tgtcgaagcc gccgtacttc ttagggtccc agtccttctt tctggcgatc agcttatcgc
     6181 tgttcctctt gggcaggata gactctttgc tgaagccgcc tgtctgcacc tcggtctttt
     6241 tcacgatatt cacttggggc atgctcagca ctttccgcac ggtggcaaaa tcccggccct
     6301 tatcccacac gatctccccg gtttcgccgt ttgtctcgat cagaggccgc ttccggatct
     6361 cgccgttggc cagggtaatc tcggtcttga aaaagttcat gatgttgctg tagaagaagt
     6421 acttggcggt agccttgccg atttcctgct cgctcttggc gatcatcttc cgcacgtcgt
     6481 acaccttgta gtcgccgtac acgaactcgc tttccagctt agggtacttt ttgatcaggg
     6541 cggttcccac gacggcgttc aggtaggcgt cgtgggcgtg gtggtagttg ttgatctcgc
     6601 gcactttgta aaactggaaa tccttccgga aatcggacac cagcttggac ttcagggtga
     6661 tcactttcac ttcccggatc agcttgtcat tctcgtcgta cttagtgttc atccgggagt
     6721 ccaggatctg tgccacgtgc tttgtgatct gccgggtttc caccagctgt ctcttgatga
     6781 agccggcctt atccagttcg ctcaggccgc ctctctcggc cttggtcaga ttgtcgaact
     6841 ttctctgggt aatcagcttg gcgttcagca gctgccgcca gtagttcttc atcttcttca
     6901 cgacctcttc ggagggcacg ttgtcgctct tgccccggtt cttgtcgctt ctggtcagca
     6961 ccttgttgtc gatggagtcg tccttcagaa agctctgagg cacgatatgg tccacatcgt
     7021 agtcggacag ccggttgatg tccagttcct ggtccacgta catatcccgc ccattctgca
     7081 ggtagtacag gtacagcttc tcgttctgca gctgggtgtt ttccacgggg tgttctttca
     7141 ggatctggct gcccagctct ttgatgccct cttcgatccg cttcattctc tcgcggctgt
     7201 tcttctgtcc cttctgggtg gtctggttct ctctggccat ttcgatcacg atgttctcgg
     7261 gcttgtgccg gcccatcact ttcacgagct cgtccaccac cttcactgtc tgcaggatgc
     7321 ccttcttaat ggcggggctg ccggccagat tggcaatgtg ctcgtgcagg ctatcgccct
     7381 ggccggacac ctgggctttc tggatgtcct ctttaaaggt caggctgtcg tcgtggatca
     7441 gctgcatgaa gtttctgttg gcgaagccgt cggacttcag gaaatccagg attgtcttgc
     7501 cggactgctt gtcccggatg ccgttgatca gcttccggct cagcctgccc cagccggtgt
     7561 atctccgccg cttcagctgc ttcatcactt tgtcgtcgaa caggtgggca taggttttca
     7621 gccgttcctc gatcatctct ctgtcctcaa acagtgtcag ggtcagcacg atatcttcca
     7681 gaatgtcctc gttttcctca ttgtccagga agtccttgtc cttgataatt ttcagcagat
     7741 cgtggtatgt gcccagggag gcgttgaacc gatcttccac gccggagatt tccacggagt
     7801 cgaagcactc gattttcttg aagtagtcct ctttcagctg cttcacggtc actttccggt
     7861 tggtcttgaa cagcaggtcc acgatggcct ttttctgctc gccgctcagg aaggcgggct
     7921 ttctcattcc ctcggtcacg tatttcactt tggtcagctc gttatacacg gtgaagtact
     7981 cgtacagcag gctgtgcttg ggcagcacct tctcgttggg caggttctta tcgaagttgg
     8041 tcatccgctc gatgaagctc tgggcggaag cgcccttgtc caccacttcc tcgaagttcc
     8101 agggggtgat ggtttcctcg ctctttctgg tcatccaggc gaatctgctg tttcccctgg
     8161 ccagagggcc cacgtagtag gggatgcgga aggtcaggat cttctcgatc ttttcccggt
     8221 tgtccttcag gaatgggtaa aaatcttcct gccgccgcag aatggcgtgc agctctccca
     8281 ggtggatctg gtgggggatg ctgccgttgt cgaaggtccg ctgcttccgc agcaggtcct
     8341 ctctgttcag cttcacgagc agttcctcgg tgccgtccat cttttccagg atgggcttga
     8401 tgaacttgta gaactcttcc tggctggctc cgccgtcaat gtagccggcg tagccgttct
     8461 tgctctggtc gaagaaaatc tctttgtact tctcaggcag ctgctgccgc acgagagctt
     8521 tcagcagggt caggtcctgg tggtgctcgt cgtatctctt gatcatagag gcgctcaggg
     8581 gggccttggt gatctcggtg ttcactctca ggatgtcgct cagcaggatg gcgtcggaca
     8641 ggttcttggc ggccagaaac aggtcggcgt actggtcgcc gatctgggcc agcaggttgt
     8701 ccaggtcgtc gtcgtaggtg tccttgctca gctgcagttt ggcatcctcg gccaggtcga
     8761 agttgctctt gaagttgggg gtcaggccca ggctcagggc aatcaggttt ccgaacaggc
     8821 cattcttctt ctcgccgggc agctgggcga tcagattttc cagccgtctg ctcttgctca
     8881 gtctggcaga caggatggcc ttggcgtcca cgccgctggc gttgatgggg ttttcctcga
     8941 acagctggtt gtaggtctgc accagctgga tgaacagctt gtccacgtcg ctgttgtcgg
     9001 ggttcaggtc gccctcgatc aggaagtggc cccggaactt gatcatgtgg gccagggcca
     9061 gatagatcag ccgcaggtcg gccttgtcgg tgctgtccac cagtttcttt ctcaggtggt
     9121 agatggtggg gtacttctcg tggtaggcca cctcgtccac gatgttgccg aagatggggt
     9181 gccgctcgtg cttcttatcc tcttccacca ggaaggactc ttccagtctg tggaagaagc
     9241 tgtcgtccac cttggccatc tcgttgctga agatctcttg cagatagcag atccggttct
     9301 tccgtctggt gtatcttctt ctggcggttc tcttcagccg ggtggcctcg gctgtttcgc
     9361 cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat gctgtgccgg tcggtgttgc
     9421 ccagcacctt gaatttcttg ctgggcacct tgtactcgtc ggtgatcacg gcccagccca
     9481 cagagttggt gccgatgtcc aggccgatgc tgtacttctt gtccatggtg gcagcgctct
     9541 agaaccggtc ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     9601 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     9661 cactttccca gtttaccccg cgccaccttc tctaggcacc ggatcaattg ccgacccctc
     9721 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc
     9781 caattcccac tcctttcaag acctagctag cgaattcaaa aaagcaccga ctcggtgcca
     9841 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaact
     9901 ggaaaacggg cagccggacc ggtgtttcgt cctttccaca agatatataa agccaagaaa
     9961 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
    10021 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
    10081 gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa
    10141 tatgaaggaa tcatgggaaa taggccctcg gtaccttaat taaccaaact ggatctctgc
    10201 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
    10261 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
    10321 cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa ttggcgcacg
    10381 cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc
    10441 ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa
    10501 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
    10561 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
    10621 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
    10681 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
    10741 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
    10801 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
    10861 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
    10921 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
    10981 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
    11041 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
    11101 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
    11161 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
    11221 atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt
    11281 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
    11341 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
    11401 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
    11461 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
    11521 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
    11581 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
    11641 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
    11701 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
    11761 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
    11821 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
    11881 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
    11941 cccagtacag gcaaaacgcg ctgcttatat agacctccca ccgtacacgc ctaccgccca
    12001 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
    12061 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
    12121 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
    12181 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
    12241 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
    12301 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
    12361 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
    12421 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
    12481 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
    12541 aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat
    12601 tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact
    12661 actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag
    12721 agcagattgt actgagagtg caccataggg gatcgggaga tctcccgatc cgtcgacgtc
    12781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    12841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    12901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    12961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctga
//
