LOCUS       Exported               13013 bp ds-DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.1.0
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(94..141)
                     /label=rEM7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        complement(189..518)
                     /label=rSV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      203..338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(532..960)
                     /direction=LEFT
                     /label=rf1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(981..1002)
                     /label=rSV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     polyA_signal    complement(1006..1230)
                     /label=rbGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     LTR             complement(1262..1495)
                     /label=r3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     CDS             complement(1497..1571)
                     /label=rnef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
     misc_feature    complement(1567..2155)
                     /label=rWPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1673..1684
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(2171..2768)
                     /label=rPuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=99.8"
                     /note="/other=CDS"
     CDS             complement(2171..2767)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             complement(2768..2824)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2834..2857)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2858..2905)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(2906..7009)
                     /label=rCas9
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     regulatory      7006..7015
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(7034..7245)
                     /label=rEF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(7271..7289)
                     /label=rsmall t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=CDS"
     misc_RNA        complement(7307..7382)
                     /label=rgRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     misc_feature    7383..7402
                     /label=ALDH1A3-gRNA2 target
     promoter        complement(7412..7652)
                     /label=rU6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7703..7820)
                     /label=rcPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(7870..7970)
                     /label=rRev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    complement(8347..8580)
                     /label=rRRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(8726..9089)
                     /label=rHIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
     misc_feature    complement(9073..9198)
                     /label=rHIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     LTR             complement(9245..9425)
                     /label=r5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     promoter        complement(9443..9642)
                     /label=rCMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=98.0"
                     /note="/other=promoter"
     promoter        complement(9443..9641)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        complement(9643..10022)
                     /label=rCMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     misc_feature    10177..10229
                     /label=rbom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
     promoter        10288..10392
                     /label=rAmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             10393..11253
                     /label=rAmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      11424..12012
                     /label=rori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             12195..12287
                     /label=rlacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    12300..12321
                     /label=rCAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        12336..12366
                     /label=rlac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    12370..12392
                     /label=rlac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     protein_bind    12374..12390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12398..12414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(12463..12584)
                     /label=rSV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(12579..12704)
                     /label=rble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=33.3"
                     /note="/other=CDS"
     CDS             complement(join(12714..13013,1..75))
                     /label=rBleoR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
       61 ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta
      121 tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc
      181 gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      241 tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      301 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      361 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      421 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      481 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt
      541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      961 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
     1021 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     1081 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     1141 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     1201 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc
     1261 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     1321 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     1381 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     1441 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     1501 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     1561 gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     1621 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     1681 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     1741 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     1801 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     1861 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     1921 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     1981 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     2041 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     2101 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga
     2161 cttaacgcgt tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     2221 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     2281 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     2341 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gactccgacg gtggccagga
     2401 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     2461 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     2521 cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca
     2581 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     2641 cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     2701 cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     2761 actcggtcgg tccaggattc tcttcgacat ctccggcttg tttcagcaga gagaagtttg
     2821 ttgcgccgga tcccttatcg tcatcgtctt tgtaatcttt cttcttctta gcctgtccag
     2881 ccttctttgt ggcggcaggt cgcttgtcgc ctcccagctg agacaggtcg atccgtgtct
     2941 cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct ttggtgctgg
     3001 tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg gctcccagat
     3061 tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc ttatcccggt
     3121 gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc actctcttgg
     3181 agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt tccacaaaca
     3241 gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg ctggccaggt
     3301 acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc agttcgccgg
     3361 cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac ttaggcagct
     3421 tgatgatcag gtcctttttc acttctttgt agcccttggc ttccagaaag tcgatgggat
     3481 tcttctcgaa gctgcttctt tccatgatgg tgatccccag cagctctttc acactcttca
     3541 gtttcttgga cttgcccttt tccactttgg ccaccaccag cacagaatag gccacggtgg
     3601 ggctgtcgaa gccgccgtac ttcttagggt cccagtcctt ctttctggcg atcagcttat
     3661 cgctgttcct cttgggcagg atagactctt tgctgaagcc gcctgtctgc acctcggtct
     3721 ttttcacgat attcacttgg ggcatgctca gcactttccg cacggtggca aaatcccggc
     3781 ccttatccca cacgatctcc ccggtttcgc cgtttgtctc gatcagaggc cgcttccgga
     3841 tctcgccgtt ggccagggta atctcggtct tgaaaaagtt catgatgttg ctgtagaaga
     3901 agtacttggc ggtagccttg ccgatttcct gctcgctctt ggcgatcatc ttccgcacgt
     3961 cgtacacctt gtagtcgccg tacacgaact cgctttccag cttagggtac tttttgatca
     4021 gggcggttcc cacgacggcg ttcaggtagg cgtcgtgggc gtggtggtag ttgttgatct
     4081 cgcgcacttt gtaaaactgg aaatccttcc ggaaatcgga caccagcttg gacttcaggg
     4141 tgatcacttt cacttcccgg atcagcttgt cattctcgtc gtacttagtg ttcatccggg
     4201 agtccaggat ctgtgccacg tgctttgtga tctgccgggt ttccaccagc tgtctcttga
     4261 tgaagccggc cttatccagt tcgctcaggc cgcctctctc ggccttggtc agattgtcga
     4321 actttctctg ggtaatcagc ttggcgttca gcagctgccg ccagtagttc ttcatcttct
     4381 tcacgacctc ttcggagggc acgttgtcgc tcttgccccg gttcttgtcg cttctggtca
     4441 gcaccttgtt gtcgatggag tcgtccttca gaaagctctg aggcacgata tggtccacat
     4501 cgtagtcgga cagccggttg atgtccagtt cctggtccac gtacatatcc cgcccattct
     4561 gcaggtagta caggtacagc ttctcgttct gcagctgggt gttttccacg gggtgttctt
     4621 tcaggatctg gctgcccagc tctttgatgc cctcttcgat ccgcttcatt ctctcgcggc
     4681 tgttcttctg tcccttctgg gtggtctggt tctctctggc catttcgatc acgatgttct
     4741 cgggcttgtg ccggcccatc actttcacga gctcgtccac caccttcact gtctgcagga
     4801 tgcccttctt aatggcgggg ctgccggcca gattggcaat gtgctcgtgc aggctatcgc
     4861 cctggccgga cacctgggct ttctggatgt cctctttaaa ggtcaggctg tcgtcgtgga
     4921 tcagctgcat gaagtttctg ttggcgaagc cgtcggactt caggaaatcc aggattgtct
     4981 tgccggactg cttgtcccgg atgccgttga tcagcttccg gctcagcctg ccccagccgg
     5041 tgtatctccg ccgcttcagc tgcttcatca ctttgtcgtc gaacaggtgg gcataggttt
     5101 tcagccgttc ctcgatcatc tctctgtcct caaacagtgt cagggtcagc acgatatctt
     5161 ccagaatgtc ctcgttttcc tcattgtcca ggaagtcctt gtccttgata attttcagca
     5221 gatcgtggta tgtgcccagg gaggcgttga accgatcttc cacgccggag atttccacgg
     5281 agtcgaagca ctcgattttc ttgaagtagt cctctttcag ctgcttcacg gtcactttcc
     5341 ggttggtctt gaacagcagg tccacgatgg cctttttctg ctcgccgctc aggaaggcgg
     5401 gctttctcat tccctcggtc acgtatttca ctttggtcag ctcgttatac acggtgaagt
     5461 actcgtacag caggctgtgc ttgggcagca ccttctcgtt gggcaggttc ttatcgaagt
     5521 tggtcatccg ctcgatgaag ctctgggcgg aagcgccctt gtccaccact tcctcgaagt
     5581 tccagggggt gatggtttcc tcgctctttc tggtcatcca ggcgaatctg ctgtttcccc
     5641 tggccagagg gcccacgtag taggggatgc ggaaggtcag gatcttctcg atcttttccc
     5701 ggttgtcctt caggaatggg taaaaatctt cctgccgccg cagaatggcg tgcagctctc
     5761 ccaggtggat ctggtggggg atgctgccgt tgtcgaaggt ccgctgcttc cgcagcaggt
     5821 cctctctgtt cagcttcacg agcagttcct cggtgccgtc catcttttcc aggatgggct
     5881 tgatgaactt gtagaactct tcctggctgg ctccgccgtc aatgtagccg gcgtagccgt
     5941 tcttgctctg gtcgaagaaa atctctttgt acttctcagg cagctgctgc cgcacgagag
     6001 ctttcagcag ggtcaggtcc tggtggtgct cgtcgtatct cttgatcata gaggcgctca
     6061 ggggggcctt ggtgatctcg gtgttcactc tcaggatgtc gctcagcagg atggcgtcgg
     6121 acaggttctt ggcggccaga aacaggtcgg cgtactggtc gccgatctgg gccagcaggt
     6181 tgtccaggtc gtcgtcgtag gtgtccttgc tcagctgcag tttggcatcc tcggccaggt
     6241 cgaagttgct cttgaagttg ggggtcaggc ccaggctcag ggcaatcagg tttccgaaca
     6301 ggccattctt cttctcgccg ggcagctggg cgatcagatt ttccagccgt ctgctcttgc
     6361 tcagtctggc agacaggatg gccttggcgt ccacgccgct ggcgttgatg gggttttcct
     6421 cgaacagctg gttgtaggtc tgcaccagct ggatgaacag cttgtccacg tcgctgttgt
     6481 cggggttcag gtcgccctcg atcaggaagt ggccccggaa cttgatcatg tgggccaggg
     6541 ccagatagat cagccgcagg tcggccttgt cggtgctgtc caccagtttc tttctcaggt
     6601 ggtagatggt ggggtacttc tcgtggtagg ccacctcgtc cacgatgttg ccgaagatgg
     6661 ggtgccgctc gtgcttctta tcctcttcca ccaggaagga ctcttccagt ctgtggaaga
     6721 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
     6781 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
     6841 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
     6901 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
     6961 ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtccatg gtggcagcgc
     7021 tctagaaccg gtcctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga
     7081 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga
     7141 catcactttc ccagtttacc ccgcgccacc ttctctaggc accggatcaa ttgccgaccc
     7201 ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg
     7261 agccaattcc cactcctttc aagacctagc tagcgaattc aaaaaagcac cgactcggtg
     7321 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
     7381 accgttagcg gtggccatgg ctcggtgttt cgtcctttcc acaagatata taaagccaag
     7441 aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt
     7501 aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg tactaatatc
     7561 tttgtgttta cagtcaaatt aattctaatt atctctctaa cagccttgta tcgtatatgc
     7621 aaatatgaag gaatcatggg aaataggccc tcggtacctt aattaaccaa actggatctc
     7681 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     7741 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     7801 tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctgcag aattggcgca
     7861 cgcagtgccg atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     7921 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     7981 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     8041 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     8101 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     8161 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     8221 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     8281 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     8341 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     8401 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     8461 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     8521 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     8581 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     8641 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     8701 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
     8761 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     8821 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     8881 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     8941 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     9001 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     9061 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     9121 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     9181 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     9241 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     9301 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     9361 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     9421 gacccagtac aggcaaaacg cgctgcttat atagacctcc caccgtacac gcctaccgcc
     9481 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     9541 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     9601 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     9661 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     9721 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     9781 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     9841 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     9901 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     9961 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
    10021 tcaacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
    10081 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
    10141 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
    10201 agagcagatt gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg
    10261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    12181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    12241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    12301 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    12361 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    12421 tacgccaagc tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt
    12481 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
    12541 ctattgcttt atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac
    12601 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
    12661 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc
    12721 tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
    12781 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
    12841 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
    12901 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
    12961 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccg
//
