LOCUS       Exported                8324 bp ds-DNA     circular SYN 20-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 25, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      911..1499
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1682..1774
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    1787..1808
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1823..1853
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1861..1877
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     1885..1901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1922..1940
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1968..2194
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             2195..2375
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    2422..2547
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2531..2894
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
     misc_feature    3040..3273
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             3650..3676
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=7.7"
                     /note="/other=CDS"
     promoter        3680..3920
                     /label=U6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3930..3949
                     /label=ALDH1A3 gRNA3 target
     misc_RNA        3950..4025
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     misc_feature    4081..4198
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4264..4330
                     /label=small t antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=12.8"
                     /note="/other=CDS"
     promoter        4337..5515
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     promoter        4356..4567
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          4568..5506
                     /label=EF-1-alpha intron A
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             5528..6128
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.2"
                     /note="/match_length=100.3"
                     /note="/other=CDS"
     misc_feature    6144..6732
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             complement(6615..6626)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6728..6802
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.0"
                     /note="/match_length=25.8"
                     /note="/other=CDS"
     LTR             6804..7037
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    7115..7236
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      7276..7411
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(7432..7450)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             7458..7613
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     primer_bind     complement(7460..7476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7618..8073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      7618..8046
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        8099..8203
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(8204..8324,1..740)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
       61 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      121 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      181 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      241 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      301 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      361 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      421 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      481 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      541 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      601 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      661 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      721 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      781 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      841 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      961 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1861 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     1921 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     1981 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     2041 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     2101 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     2161 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     2221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2341 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     2401 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     2461 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     2521 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     2581 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     2641 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     2701 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     2761 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2821 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2881 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2941 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     3001 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     3061 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     3121 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     3181 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     3241 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     3301 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3361 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3421 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3481 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3541 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3601 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3661 aaccccgagg ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     3721 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
     3781 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     3841 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     3901 atatatcttg tggaaaggac gaaacaccgt gaacttgacc tccaggttgg ttttagagct
     3961 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4021 ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa
     4081 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4141 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4201 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca
     4261 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     4321 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4381 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     4441 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4501 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4561 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     4621 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     4681 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     4741 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     4801 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     4861 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     4921 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     4981 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     5041 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     5101 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     5161 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     5221 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     5281 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     5341 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     5401 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     5461 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac
     5521 ggccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5581 tggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5641 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5701 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
     5761 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5821 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5881 ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg
     5941 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6001 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6061 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6121 gtgcctgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6181 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6241 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6301 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6361 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6421 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6481 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6541 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6601 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6661 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6721 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     6781 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6841 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6901 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6961 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7021 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     7081 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7261 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7321 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7381 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     7441 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     7501 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7561 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7621 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7681 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7741 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7801 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7861 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7921 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7981 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8041 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     8101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8221 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8281 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcac
//
