LOCUS       Exported                5986 bp ds-DNA     circular SYN 19-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   CIM-Functional Validation Core
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 20, 2022 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    complement(6..196)
                     /label=ITR(R)
                     /note="Tol2 ITR(R)"
     misc_recomb     248..281
                     /label=LoxP
     misc_feature    288..420
                     /label=BP/SA
                     /note="carp beta actin splice acceptor from intron 1/exon2 
                     boundary"
     primer_bind     complement(416..437)
                     /label=MISC-bact_intron-R1
     CDS             453..1127
                     /label=mRFP*
                     /note="mRFP with AUG removed"
     primer_bind     complement(1106..1134)
                     /label=CDS-mRFP-R1
     polyA_signal    1136..2311
                     /label=ocean pout terminator
                     /note="3'UTR, poly(A) signal, and putative boundary element
                     from ocean pout (FRM- Pat Gibbs)."
     misc_feature    2355..2715
                     /label=gamma-crystallin
                     /note="Xenopus"
     exon            2744..2767
                     /label=beta actin exon 1
                     /note="carp beta actin exon 1 (non-coding)"
     intron          2768..2965
                     /label=beta-actin intron 1
                     /note="carp beta actin intron 1"
     primer_bind     complement(2956..2983)
                     /label=MISC-bact_intron-R1
     misc_signal     2986..3015
                     /label=NLS
     CDS             3016..3717
                     /label=tagBFP
     misc_feature    3736..3863
                     /label=SD
                     /note="carp beta actin splice-donor exon/intron 1 boundary"
     misc_recomb     3883..3916
                     /label=loxP
     misc_feature    3973..4190
                     /label=ITR(L)
                     /note="Tol2 ITR(L)"
     CDS             4255..5064
                     /label=Kan(R)
     rep_origin      5097..5978
                     /label=ColE1 Ori
ORIGIN
        1 ggtaccgagc agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac
       61 ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa
      121 aagtaaaaaa gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca
      181 agaaagaaaa ctagagtggt ctccctttag tgagggttaa ttgatgggat agggataaca
      241 gggtaatata acttcgtata gcatacatta tacgaagtta tcgttaccac ccactagcgg
      301 tcagactgca gattgcagca cgaaacagga agctgactcc acatggtcac atgctcactg
      361 aagtgttgac ttccctgaca gctgtgcact ttctaaaccg gttttctcat tcatttacag
      421 ttcagccgga attcacccgg gtaccggaag gtgcctcctc cgaggacgtc atcaaggagt
      481 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      541 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      601 gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttccagtac ggctccaagg
      661 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
      721 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
      781 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
      841 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcggatgt
      901 accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg aaggacggcg
      961 gccactacga cgccgaggtc aagaccacct acatggccaa gaagcccgtg cagctgcccg
     1021 gcgcctacaa gaccgacatc aagctggaca tcacctccca caacgaggac tacaccatcg
     1081 tggaacagta cgagcgcgcc gagggccgcc actccaccgg agcctaagac tagtaattaa
     1141 gtctcagcca ccgttaactg aacatgtcaa aacctgtgga gactgttgag atttgatgtt
     1201 ctgaaaagat aaagcctata aataaaatgt tgcccaaatt tcctgcctga tgtttttctt
     1261 tgtctttgct acatggcttt gctgctcgga tcggctcact ctgtgtatgc cacgttcact
     1321 ttgtactctc cttctcacgg taggtttatt atttttagat gtgcagttag tttctgtgaa
     1381 ataacacacc acacactgat attgtctgtg cattgacttg gtgagtgcac attgtttttg
     1441 atcttgacat atttatattt gattgatcag gtgaactgtg tgaatctaaa gtgctccata
     1501 cagatgttct gcattgaaaa tattctcatt ttattagtgg aagtgagtgt atgccacatc
     1561 caatcaattt cagcaaacac cccagtatga tttaatgcaa aaaaatgaag gtatcaaaca
     1621 cgcattacta ctttgcagtt aaatatttaa catttattcc aacacgaaaa aaagcagtaa
     1681 ataacacttt gacaaacacg tcaggacatc ttatttttgt caccctcaca ggcaatttag
     1741 tataatatat tatatatata tatatatcat ataataatat tcagtataat atatatatat
     1801 atatcatatt ataatattca gtataatata aaacacaaac acatatatgt ataatataat
     1861 ataacatttt tatttattga gatgcctcta tggaccgtgt tataagaagt aaagatcagg
     1921 agaagtaaac atgaagtgta attatgaata ctgatgttaa attaagctat gatgagtttt
     1981 cactgttaat ttaccatctc aattaaatgt tgatgcctcc atgaccaagt taagcagatg
     2041 agactgagac aactgtagaa gacaagatgt tcactttgct gaatatagct ggcttgacag
     2101 ttatctatga ctctataaat atatatatat tttttttttt ataaaatgat ttatttataa
     2161 ctatatatcc atttctcaga caggtgcttc atatccctca ctcccgtagc tgtccatgct
     2221 ggatctgtcc ccgttgtttt taaaaagcta aataagttat taacatgact gcatccagcg
     2281 agccaaacct gtctggtgta cagctaccag agaagcttga gatcctagtc accggcgttc
     2341 ctagttcttt aaactgttac aaagtgtttg gaccagcaac tgaattgcac tgtaaattac
     2401 cacatcaggg tctccggggt caacgctgct aagcaatgga ctggaaaatt tgtgacattg
     2461 agtttttgga ataactggga atgctggata actctttggg taaagctatg gaacaaaagt
     2521 accagcatga actaagaaat gactgtcaca gcacaacatc aaacacaatg caagacaaag
     2581 gttctctgct tttgccttac ttgacagaaa aagtaagctc taggttcatc gcatgcagac
     2641 agcagcaata catgacatat ataaaggctg gctcttttct cccttccatt gaactgaaac
     2701 ttccactcag tcagacttgc gggggatcca cagcctgact aactcgacaa acccccccaa
     2761 acctaaggtg agttgatctt taagcttttt acattttcag ctcgcatata tcaattcgcg
     2821 cgcattgtca cactagcacc cactagcggt cagactgcag acaaacagga agctgactcc
     2881 acatggtcac atgctcactg aagtgttgac ttccctgaca gctgtgcact ttctaaaccg
     2941 gttttctcat tcatttacag ttcagccgga attcacctcg agaccatggc tccaaagaag
     3001 aagcgtaagg taaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     3061 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     3121 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     3181 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     3241 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     3301 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     3361 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     3421 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     3481 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag
     3541 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     3601 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     3661 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct caattaaagc
     3721 tgagatctac ctaaggtgag ttgatcttta agctttttac attttcagct cgcatatatc
     3781 aattcgaacg tttaattaga atgtttaaat aaagctagat taaatgatta ggctcagtta
     3841 ccggtctttt ttttctcatt tacactgagc tcaagacgtc tgataacttc gtatagcata
     3901 cattatacga agttattacc ctgttatccc tatggctagc gatgggatct aatacgactc
     3961 actataggga gaccaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     4021 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     4081 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     4141 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg ggatccagct
     4201 gcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     4261 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     4321 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     4381 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     4441 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     4501 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     4561 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     4621 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     4681 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     4741 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     4801 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     4861 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     4921 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     4981 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     5041 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcaaaaa
     5101 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5581 gatacctaca gcgtgagcta tgagaaagcg ccacggttcc cgaagggaga aaggcggaca
     5641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5821 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5881 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5941 ccgagcgcag cgagtcagtg agcgaggaag cggaacagat ttaaat
//
