LOCUS       p15A-OptoCre-knt-Ps-R   3410 bp ds-DNA     circular     22-NOV-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..71
                     /label="P*"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     72..105
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    145..273
                     /label="rrnBT1-T7TE terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_recomb     276..309
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     RBS             310..329
                     /label="R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    330..1124
                     /label="knt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             330..1124
                     /label="Translation 330-1124"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    1137..1265
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1272..1983)
                     /label="p15a"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     terminator      2074..2179
                     /label="TO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(2195..2854)
                     /label="cmR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(2855..2957)
                     /label="PcmR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 TTATCAAAAA GAGTATTGTC TTAAAGTCTA ACCTATAGGA TTCTTACAGC CATCGAGAGG
       61 GACACGGCGA Aataacttcg tatagcatac attatacgaa gttatccagg catcaaataa
      121 ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
      181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      241 gctcaccttc gggtgggcct ttctgcgttt ataccataac ttcgtatagc atacattata
      301 cgaagttatT TTAAGAAGGA GATATACATa tgattgaaca agatggattg cacgcaggtt
      361 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
      421 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
      481 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
      541 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
      601 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
      661 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
      721 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
      781 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
      841 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
      901 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
      961 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1021 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     1081 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgataatag ggatctccag
     1141 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1201 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1261 ttatacctag ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1321 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     1381 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     1441 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     1501 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     1561 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     1621 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     1681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     1741 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     1801 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     1861 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     1921 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     1981 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
     2041 agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc
     2101 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     2161 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     2221 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     2281 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     2341 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2401 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2461 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2521 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2581 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2641 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2701 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2761 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2821 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2881 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2941 ctcttacgtg ccgatcaacg tctcattttc gccagatatc gacaattccg gttcgcttgc
     3001 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     3061 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     3121 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     3181 ccctgagtgc ttgcggcagc gtgagcttca aaagcgctct gaagttccta tactttctag
     3241 agaataggaa cttcgaactg caggtcgacg gatccccgga attaattctc atgtttgaca
     3301 ggatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     3361 gtgatgccgg ccacgatgcc gaagttccta ttatcacgag gccctttgag
//