LOCUS       p15A-OptoCre-knt-P-Rs   3217 bp ds-DNA     circular     22-NOV-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..71
                     /label="P"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     72..105
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    145..273
                     /label="rrnBT1-T7TE terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_recomb     276..309
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    310..333
                     /label="R*"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    334..1128
                     /label="knt"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             334..1128
                     /label="Translation 334-1128"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    1141..1269
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1276..1987)
                     /label="p15a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      2078..2183
                     /label="TO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(2199..2858)
                     /label="cmR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        complement(2859..2961)
                     /label="PcmR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 TTATCAAAAA GAGTATTGCA TTAAAGTCTA ACCTATAGGA ATCTTACAGC CATCGAGAGG
       61 GACACGGCGA Aataacttcg tatagcatac attatacgaa gttatccagg catcaaataa
      121 ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
      181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      241 gctcaccttc gggtgggcct ttctgcgttt ataccataac ttcgtatagc atacattata
      301 cgaagttatA TCACTCTACG GCCAGCTGCA AACatgattg aacaagatgg attgcacgca
      361 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      421 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
      481 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
      541 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
      601 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
      661 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
      721 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
      781 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
      841 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
      901 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
      961 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     1021 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     1081 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgata atagggatct
     1141 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1201 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1261 gcgtttatac ctagggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     1321 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     1381 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     1441 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     1501 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     1561 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     1621 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     1681 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     1741 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     1801 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     1861 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     1921 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     1981 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac
     2041 ctgaagtcag ccccatacga tataagttgt tactagtgct tggattctca ccaataaaaa
     2101 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2161 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     2221 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     2281 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2341 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2401 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     2461 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2521 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     2581 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     2641 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     2701 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     2761 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     2821 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     2881 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     2941 gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc ggtgcctaat
     3001 gagtgagcta acttacatta attgcgttgc gcattcacca ccctgaattg actctcttcc
     3061 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgga tcttccccat
     3121 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     3181 cgatgccgaa gttcctatta tcacgaggcc ctttgag
//