LOCUS       p15A-OptoCre-cat-P-R    3427 bp ds-DNA     circular     12-SEP-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..71
                     /label="P"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     72..105
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    145..273
                     /label="rrnBT1-T7TE terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_recomb     276..309
                     /label="LoxP"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     RBS             310..329
                     /label="RBS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             330..989
                     /label="cat"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             330..989
                     /label="Translation 330-989"
     misc_feature    1002..1130
                     /label="dbl term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1137..1848)
                     /label="p15a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     terminator      1939..2044
                     /label="TO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2070..2864)
                     /label="kanR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 TTATCAAAAA GAGTATTGCA TTAAAGTCTA ACCTATAGGA ATCTTACAGC CATCGAGAGG
       61 GACACGGCGA Aataacttcg tatagcatac attatacgaa gttatccagg catcaaataa
      121 ggatccaaac tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag
      181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      241 gctcaccttc gggtgggcct ttctgcgttt ataccataac ttcgtatagc atacattata
      301 cgaagttatT TTAAGAAGGA GATATACATa tggagaaaaa aatcactgga tataccaccg
      361 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
      421 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
      481 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
      541 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
      601 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
      661 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
      721 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
      781 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
      841 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
      901 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
      961 actgcgatga gtggcagggc ggggcgtaat aatagggatc tccaggcatc aaataaaacg
     1021 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1081 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cctagggata
     1141 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
     1201 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
     1261 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     1321 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     1381 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     1441 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
     1501 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
     1561 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
     1621 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
     1681 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
     1741 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
     1801 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
     1861 tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg
     1921 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1981 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     2041 aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     2101 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     2161 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     2221 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     2281 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     2341 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     2401 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     2461 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     2521 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     2581 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     2641 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     2701 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     2761 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     2821 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     2881 cctgtctctt gatcagatca tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     2941 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     3001 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgcat tcaccaccct
     3061 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     3121 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     3181 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     3241 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     3301 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     3361 ccgcacctgt ggcgccggtg atgccggcca cgatgccgaa gttcctatta tcacgaggcc
     3421 ctttgag
//