LOCUS       Exported                8409 bp ds-DNA     circular SYN 29-AUG-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Biology Department
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    4849..4855
                     /label=5XUAS-hsp70
                     /note="color: #ff9900"
     primer_bind     4855..4898
                     /label=2X-UAS-FP
                     /note="color: black; sequence: 
                     gtAAGCTTcggagtactgtcctccgagcggagtactgtcctccgag"
     primer_bind     4855..4879
                     /label=1X-UAS-FP
                     /note="color: black; sequence: gtAAGCTTcggagtactgtcctccgag"
     misc_feature    4861..5159
                     /label=5XUAS-hsp70
                     /note="color: #ff9900"
     misc_feature    4861..4898
                     /label=5X-UAS
                     /note="color: #a6acb3"
     primer_bind     4861..4898
                     /label=3X-UAS-FP
                     /note="color: black; sequence: 
                     gtAAGCTTcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcc
                     tccgag"
     primer_bind     4861..4898
                     /label=4X-UAS-FP
                     /note="color: black; sequence: 
                     gtAAGCTTcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcc
                     tccgagcggagtactgtcctccgag"
     misc_feature    4861..4879
                     /label=19-mer-UAS sequence
                     /note="color: #a6acb3"
     primer_bind     4861..4879
                     /label=2X-UAS-FP
                     /note="color: black; sequence: 
                     gtAAGCTTcggagtactgtcctccgagcggagtactgtcctccgag"
     misc_feature    4880..4898
                     /label=19-mer-UAS sequence
                     /note="color: #a6acb3"
     misc_feature    4917..5155
                     /label=HSP-70
                     /note="color: #a6acb3"
     primer_bind     5099..5119
                     /label=Seq-UASattB-5'
                     /note="color: black; sequence: actgcaactactgaaatctgc"
     primer_bind     complement(5135..5165)
                     /label=X-UAS-RP
                     /note="color: black; sequence: 
                     gtGAATTCtattcagagttctcttcttgtattc"
     misc_feature    5161..5207
                     /label=MCS
                     /note="color: #a6acb3"
     misc_feature    5206..5905
                     /label=SV40 poly(A)
                     /note="color: #ffff00"
     primer_bind     complement(5249..5271)
                     /label=UASTattB-seq-3'
                     /note="color: black; sequence: aaatctctgtaggtagtttgtcc"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactctag cgagcgccgg
     4921 agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa
     4981 cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct
     5041 gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac
     5101 tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag
     5161 aattcgttaa cagatctgcg gccgcggctc gagggtacct ctagaggatc tttgtgaagg
     5221 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     5281 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     5341 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     5401 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     5461 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     5521 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     5581 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     5641 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     5701 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5761 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5881 aatgtatctt atcatgtctg gatccactag tgtcgacgat gtaggtcacg gtctcgaagc
     5941 cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca
     6001 tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc
     6061 gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac
     6121 gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca
     6181 cggcgggcat gtcgacacta gttctagcca gcttttgttc cctttagtga gggttaattt
     6241 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6301 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6361 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6421 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6841 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7081 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7381 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7441 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7501 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7561 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7621 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7681 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7741 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7801 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7861 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7921 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7981 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8041 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8101 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8161 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8221 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8281 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8341 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8401 aagtgccac
//
