LOCUS       Exported               10879 bp DNA     circular SYN 23-MAR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2022 from SnapGene 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    5398..5422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5422..6863
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors human AKT1 CCDS9994.1"
                     /label=Akt1 (CCDS9994.1)
                     /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK
                     VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR
                     LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLM
                     LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY
                     EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA
                     KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM
                     ECVDSERRPHFPQFSYSASGTA"
     primer_bind     complement(6847..6886)
                     /label=attB Insert.REV
     protein_bind    complement(6863..6887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6875..6880
                     /label=BsrGI-dual Cutsite
                     /note="For cutting in the insert flanking attB, L, P sites 
                     (and the cddB gene)"
     CDS             6889..6930
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6931..6936
                     /codon_start=1
                     /label=Gap Seq
                     /translation="AS"
     CDS             6937..7707
                     /codon_start=1
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(7713..7733)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7762..8350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7815..7835)
                     /label=wPRE-R SeqRevPrimer
     CDS             complement(8233..8244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8363..8862
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             8883..9482
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             9620..9853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9925..10046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10065..10200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10233..10251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10261..10277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10270..10292)
                     /label=pDONR221-3 SeqPrimerFwd
     rep_origin      10422..10877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacaca
     5401 agtttgtaca aaaaagttgg catgagcgac gtggctattg tgaaggaggg ttggctgcac
     5461 aaacgagggg agtacatcaa gacctggcgg ccacgctact tcctcctcaa gaatgatggc
     5521 accttcattg gctacaagga gcggccgcag gatgtggacc aacgtgaggc tcccctcaac
     5581 aacttctctg tggcgcagtg ccagctgatg aagacggagc ggccccggcc caacaccttc
     5641 atcatccgct gcctgcagtg gaccactgtc atcgaacgca ccttccatgt ggagactcct
     5701 gaggagcggg aggagtggac aaccgccatc cagactgtgg ctgacggcct caagaagcag
     5761 gaggaggagg agatggactt ccggtcgggc tcacccagtg acaactcagg ggctgaagag
     5821 atggaggtgt ccctggccaa gcccaagcac cgcgtgacca tgaacgagtt tgagtacctg
     5881 aagctgctgg gcaagggcac tttcggcaag gtgatcctgg tgaaggagaa ggccacaggc
     5941 cgctactacg ccatgaagat cctcaagaag gaagtcatcg tggccaagga cgaggtggcc
     6001 cacacactca ccgagaaccg cgtcctgcag aactccaggc accccttcct cacagccctg
     6061 aagtactctt tccagaccca cgaccgcctc tgctttgtca tggagtacgc caacgggggc
     6121 gagctgttct tccacctgtc ccgggagcgt gtgttctccg aggaccgggc ccgcttctat
     6181 ggcgctgaga ttgtgtcagc cctggactac ctgcactcgg agaagaacgt ggtgtaccgg
     6241 gacctcaagc tggagaacct catgctggac aaggacgggc acattaagat cacagacttc
     6301 gggctgtgca aggaggggat caaggacggt gccaccatga agaccttttg cggcacacct
     6361 gagtacctgg cccccgaggt gctggaggac aatgactacg gccgtgcagt ggactggtgg
     6421 gggctgggcg tggtcatgta cgagatgatg tgcggtcgcc tgcccttcta caaccaggac
     6481 catgagaagc tttttgagct catcctcatg gaggagatcc gcttcccgcg cacgcttggt
     6541 cccgaggcca agtccttgct ttcagggctg ctcaagaagg accccaagca gaggcttggc
     6601 gggggctccg aggacgccaa ggagatcatg cagcatcgct tctttgccgg tatcgtgtgg
     6661 cagcacgtgt acgagaagaa gctcagccca cccttcaagc cccaggtcac gtcggagact
     6721 gacaccaggt attttgatga ggagttcacg gcccagatga tcaccatcac accacctgac
     6781 caagatgaca gcatggagtg tgtggacagc gagcgcaggc cccacttccc ccagttctcc
     6841 tactcggcca gcggcacggc ctgcccaact ttcttgtaca aagtgggcgg caagcccatc
     6901 cccaaccccc tgctgggcct ggacagcacc gctagcatcc cgctgctgaa cgctaaacag
     6961 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat
     7021 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat
     7081 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg
     7141 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc
     7201 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa
     7261 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc
     7321 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg
     7381 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac
     7441 ctggacagga atactctggc cgctatgctg atccgagagc tgcgggcagc cctggaactg
     7501 ttcgagcagg aaggcctggc tccatatctg tcacggtggg agaagctgga taacttcatc
     7561 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt
     7621 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga
     7681 gaaatcagtc tgcggtctgc cgaaaagtaa gccaacttta ttatacatag ttgatatcca
     7741 gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact
     7801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     8161 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8341 gcctccccgc ctggaattca ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     8401 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     8461 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     8521 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     8581 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     8641 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     8701 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     8761 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     8821 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     8881 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8941 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9001 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9061 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     9121 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9181 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9241 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9301 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9361 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9421 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     9481 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
     9541 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9601 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
     9661 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc
     9721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9841 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9901 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9961 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10021 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
    10081 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    10141 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    10201 taggcttttg cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
    10261 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
    10321 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
    10381 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc
    10441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    10861 attaacgttt acaatttcc
//
