LOCUS       CMV-SauriABE8e          7299 bp ds-DNA     circular     27-JUL-2022
DEFINITION  .
ACCESSION   urn.local...c-8wnwdza
VERSION     urn.local...c-8wnwdza
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     15..39
                     /standard_name="ACK11"
     misc_feature    complement(273..1133)
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     primer_bind     514..538
                     /standard_name="ACK12"
     primer_bind     679..698
                     /standard_name="ACK563"
     primer_bind     1014..1038
                     /standard_name="ACK13"
     regulatory      complement(1175..1203)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     regulatory      1524..2112
                     /note="Geneious type: promoter"
                     /standard_name="CMV_Full"
     regulatory      2031..2112
                     /note="Geneious type: Promoter"
                     /standard_name="CMV prom"
     regulatory      2133..2151
                     /note="Geneious type: Promoter"
                     /standard_name="T7 prom"
     primer_bind     2134..2156
                     /standard_name="AR397"
     misc_feature    2159..2181
                     /note="Geneious type: primer"
                     /note="sequence: gccaccatgaaacggacagccga"
                     /standard_name="jrd_793_annealing"
     CDS             2165..6064
                     /standard_name="ABE"
     gene            2165..2221
                     /standard_name="BPNLS"
     primer_bind     2218..2243
                     /standard_name="KZ1433"
     gene            2222..2719
                     /standard_name="TadAmax10 (ABE8)"
     primer_bind     2714..2739
                     /standard_name="KZ1427"
     gene            2720..2815
                     /standard_name="32aa linker"
     gene            2816..5998
                     /standard_name="nSauriCas9max"
     primer_bind     2829..2851
                     /standard_name="AR901"
     variation       2858..2860
                     /standard_name="D15A"
     primer_bind     3131..3151
                     /standard_name="AR912"
     primer_bind     3795..3813
                     /standard_name="AR913"
     primer_bind     4444..4462
                     /standard_name="AR914"
     variation       4577..4579
                     /standard_name="N588"
     primer_bind     5085..5104
                     /standard_name="AR915"
     primer_bind     5985..6018
                     /standard_name="AR903"
     gene            5999..6010
                     /standard_name="4aa linker"
     gene            6011..6061
                     /standard_name="bpNLS"
     primer_bind     6024..6046
                     /standard_name="KZ1406"
     misc_feature    complement(6038..6064)
                     /note="Geneious type: primer"
                     /note="sequence: ttagactttcctcttcttcttgggctc"
                     /standard_name="JRD_796_annealing"
     regulatory      6113..6340
                     /note="Geneious type: Terminator"
                     /standard_name="bGH_PA term"
     regulatory      complement(6460..6489)
                     /note="Geneious type: Promoter"
                     /standard_name="lac prom"
     primer_bind     6504..6528
                     /standard_name="ACK21"
     misc_feature    complement(6798..118)
                     /standard_name="pBR322 origin"
     primer_bind     6813..6837
                     /standard_name="ACK10"
     primer_bind     7036..7054
                     /standard_name="QB0766 RepO-F"
     primer_bind     7133..7152
                     /standard_name="833_BR322-rev1"
ORIGIN      
        1 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
       61 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      121 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1261 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
     1321 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     1381 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     1441 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     1501 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1561 catagcccat atattgagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1621 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1681 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1741 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1801 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1861 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     1921 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     1981 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2041 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     2101 aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc
     2161 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
     2221 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     2281 ggcacgggat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     2341 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     2401 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     2461 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     2521 cgtggtgttt ggcgtgagga actcaaaaag aggcgccgca ggctccctga tgaacgtgct
     2581 gaactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     2641 tgccgccctg ctgtgcgatt tctatcggat gcctagacag gtgttcaatg ctcagaagaa
     2701 ggcccagagc tccatcaact ccggaggatc tagcggaggc tcctctggct ctgagacacc
     2761 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcaatgca
     2821 ggagaaccag cagaagcaaa attacatcct gggcctggcc atcggcatca ccagcgtggg
     2881 ctatggcctg atcgacagca agaccagaga agtgattgac gccggcgtgc ggctattccc
     2941 agaggccgac tctgaaaaca acagcaatag aagatctaag cggggcgccc ggagactgaa
     3001 aagacgaaga atccacagac tgaacagagt gaaagacctc ctggctgact accagatgat
     3061 cgacttaaac aacgtgccca agtctaccga cccctacacc atccgggtga agggactgcg
     3121 ggaacctctg accaaggaag agtttgccat cgctctgctg catatcgcca agagaagagg
     3181 cctgcacaac atctccgtga gcatgggcga tgaggaacag gacaacgagc tgtccaccaa
     3241 gcagcagctg cagaagaacg ctcagcagct gcaggacaaa tacgtgtgcg agctgcaact
     3301 ggaaagactg accaacatca acaaggtcag aggcgagaag aaccggttca agaccgaaga
     3361 tttcgtgaag gaagtgaagc agctgtgcga gacccagcgg cagtaccaca acatcgacga
     3421 tcagttcatc cagcagtaca tcgacctggt gagcacccgg agagaatact tcgagggccc
     3481 tggcaacggc tctccatatg gctgggatgg agatctgctg aagtggtatg agaagctgat
     3541 gggcagatgc acctacttcc ctgaggagct gagaagcgtg aagtacgcct acagcgccga
     3601 tctgtttaac gccctgaacg atctgaacaa cctggttgtg acccgcgacg acaaccctaa
     3661 gctggaatac tacgagaaat accacattat cgagaacgtg ttcaagcaga agaaaaatcc
     3721 aactctgaag caaatcgcca aagagatcgg cgtgcaggat tacgacatca gaggatacag
     3781 aattaccaag tccggtaagc ctcagttcac cagcttcaaa ctctaccacg acctgaaaaa
     3841 catctttgaa caggccaaat acctggaaga tgtggagatg ctggatgaga tagctaaaat
     3901 cctgacaatc taccaagacg agatcagcat caagaaagcc ctggaccagc tgcctgagct
     3961 gctgaccgag agcgaaaaaa gccagatcgc tcagctgacc ggctacaccg gtacacatag
     4021 actgtctctg aagtgcatcc acatcgtgat cgacgagctg tgggagagcc ccgaaaacca
     4081 gatggaaatc ttcaccagac tgaacctcaa gccaaagaag gtggaaatga gcgagatcga
     4141 cagcatccct accacactgg tggatgagtt catcctgagc cctgtggtga agcgggcctt
     4201 catccagtcc atcaaggtga tcaacgctgt gatcaacaga ttcggcctgc ccgaggacat
     4261 cattatcgag ctggccagag agaagaacag caaggacaga aggaagttca tcaacaagct
     4321 gcagaaacag aatgaggcca cccggaaaaa aatcgagcag ctgctggcca agtacggcaa
     4381 taccaatgcc aagtacatga tcgaaaagat caagctgcat gacatgcagg agggcaagtg
     4441 tctgtacagc ctggaagcta tccccctgga agacctgctg tctaatccta cacactacga
     4501 ggtggaccac atcatcccta gaagcgtgtc cttcgacaac agcctgaaca acaaggttct
     4561 ggtgaagcaa agcgagaaca gcaagaaggg caataggacc ccttaccagt acctgagcag
     4621 caacgagtcc aagatctctt acaaccagtt caagcagcac attctgaacc tgtctaaggc
     4681 caaagataga atcagcaaga agaaacgaga tatgctgctg gaagaacggg acatcaacaa
     4741 attcgaggtg cagaaggaat tcatcaacag aaaccttgtg gacacccggt acgccactcg
     4801 ggagctgagc aacctgctga agacctactt cagcacacac gactacgccg tgaaagtgaa
     4861 gaccatcaac ggcggcttca caaaccacct gaggaaggtg tgggacttca agaagcaccg
     4921 gaaccacggc tacaagcacc acgccgagga tgccctggtc atcgccaacg ccgactttct
     4981 gttcaaaacc cacaaggccc tgagacggac agacaagatc ctggaacagc ctggactgga
     5041 agtcaacgac accaccgtga aggtggacac agaggagaag taccaggagt tattcgagac
     5101 accgaaacaa gtgaagaaca tcaagcagtt tagagatttc aagtattctc acagagttga
     5161 caagaagccc aaccggcagc tgatcaatga taccctgtac agtaccagag agatcgatgg
     5221 cgaaacctac gtggtccaaa cactgaaaga cctgtacgcc aaggacaatg aaaaggtgaa
     5281 aaagctcttt acagaacggc ctcaaaagat actgatgtac cagcacgatc ctaagacctt
     5341 tgagaaactg atgaccattc tgaatcagta cgctgaggca aagaatcctc tggccgctta
     5401 ttacgaggat aagggcgaat acgtgaccaa gtacgccaag aagggcaacg gccctgccat
     5461 ccacaagatc aaatacatcg acaagaaact gggcagctac ctggacgtga gtaacaaata
     5521 tcctgagaca cagaacaagc tggtgaaact gtctctgaag agctttagat tcgacatcta
     5581 caaatgtgaa cagggctaca agatggtgtc cattggctac ctcgacgtac tgaagaagga
     5641 caactactac tacatcccca aagataagta cgaggccgag aagcagaaaa agaagatcaa
     5701 ggaaagcgac ctcttcgtgg gcagcttcta ctacaacgac ctgatcatgt acgaggacga
     5761 actcttccgg gtgatcggag tgaactccga tatcaacaac ctggttgagc tgaatatggt
     5821 cgacatcacc tacaaggatt tctgcgaggt gaacaacgtg acaggcgaga agagaatcaa
     5881 gaaaaccatc ggaaagagag tggtgctgat cgagaagtat acgaccgaca tcctgggaaa
     5941 tctgtataaa acgcccctgc ctaagaagcc ccagctcatt ttcaagagag gcgagctgtc
     6001 tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
     6061 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     6121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6361 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6481 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7261 tttggtatct gcgctctgct gaagccagtt accttcgga
//
