LOCUS       Exported               11410 bp ds-DNA     circular SYN 22-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCRISPRai
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 22, 2022 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    123..156
                     /note="loxP"
     misc_feature    123..156
                     /note="loxP"
     enhancer        181..599
                     /note="hCMV enh"
     promoter        600..1918
                     /note="rEF1 prom"
     protein_bind    1968..1992
                     /gene="mutant version of lambda attB"
                     /bound_moiety="BP Clonase"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2002..6117
                     /codon_start=1
                     /note="dCas9"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDGTGG"
     CDS             4589..4600
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     CDS             6118..6138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             6139..6228
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             6235..6300
                     /codon_start=1
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             6235..6300
                     /codon_start=1
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             6334..7758
                     /codon_start=1
                     /note="MS2-P65-HSF1-GFP"
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSAGGGGSGGGGSGGGGSGPKKKRKVAAAGSPSG
                     QISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAG
                     EGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAE
                     PMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQIS
                     SSGQGGGGSGFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAE
                     NSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISL
                     LTGSEPPKAKDPTVSAA"
     misc_feature    6334..6723
                     /note="MCP (MS2 coat protein)"
     CDS             6778..6798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     misc_feature    6814..7365
                     /note="p65"
     misc_feature    7380..7752
                     /note="HSF1"
     CDS             7771..7836
                     /codon_start=1
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             7771..7836
                     /codon_start=1
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             7852..7872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             7873..8106
                     /codon_start=1
                     /note="COM"
                     /translation="GSMKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPT
                     EKHCGKREKITHSDETVRYGSTSGHKLNGGGGG"
     CDS             8107..8325
                     /codon_start=1
                     /note="KRAB"
                     /translation="MDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLE
                     NYKNLVSLGYQLTKPDVILRLEKGEEP"
     gene            8343..8921
                     /note="WPRE"
     polyA_signal    8953..9193
                     /note="SV40 pA"
     misc_feature    9235..9268
                     /note="loxP"
     misc_feature    9235..9268
                     /note="loxP"
     polyA_signal    9301..9324
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    9302..9316
                     /note="GT rich"
     rep_origin      complement(9583..10171)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10342..11202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11203..11307)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
       61 aagctacaac aaggcaaggc ttgaccgaca attgtaagaa ttcatctccc ggtaccgcat
      121 gcataacttc gtataatgta tgctatacga agttatgcta gcaccatggc tcgagttctt
      181 aattaacctg caggcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      481 gtcatcgcta ttaccatgat gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      541 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttga
      601 ctagtggagc cgagagtaat tcatacaaaa ggagggatcg ccttcgcaag gggagagccc
      661 agggaccgtc cctaaattct cacagaccca aatccctgta gccgccccac gacagcgcga
      721 ggagcatgcg cccagggctg agcgcgggta gatcagagca cacaagctca cagtccccgg
      781 cggtgggggg aggggcgcgc tgagcggggg ccagggagct ggcgcggggc aaactgggaa
      841 agtggtgtcg tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtatataagt
      901 gcggtagtcg ccttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt
      961 ggcgggtgtg gcttccgcgg gccccggagc tggagccctg ctctgagcgg gccgggctga
     1021 tatgcgagtg tcgtccgcag ggtttagctg tgagcattcc cacttcgagt ggcgggcggt
     1081 gcgggggtga gagtgcgagg cctagcggca accccgtagc ctcgcctcgt gtccggcttg
     1141 aggcctagcg tggtgtccgc cgccgcgtgc cactccggcc gcactatgcg ttttttgtcc
     1201 ttgctgccct cgattgcctt ccagcagcat gggctaacaa agggagggtg tggggctcac
     1261 tcttaaggag cccatgaagc ttacgttgga taggaatgga agggcaggag gggcgactgg
     1321 ggcccgcccg ccttcggagc acatgtccga cgccacctgg atggggcgag gcctgtggct
     1381 ttccgaagca atcgggcgtg agtttagcct acctgggcca tgtggcccta gcactgggca
     1441 cggtctggcc tggcggtgcc gcgttccctt gcctcccaac aagggtgagg ccgtcccgcc
     1501 cggcaccagt tgcttgcgcg gaaagatggc cgctcccggg gccctgttgc aaggagctca
     1561 aaatggagga cgcggcagcc cggtggagcg ggcgggtgag tcacccacac aaaggaagag
     1621 ggccttgccc ctcgccggcc gctgcttcct gtgaccccgt ggtctatcgg ccgcatagtc
     1681 acctcgggct tctcttgagc accgctcgtc gcggcggggg gaggggatct aatggcgttg
     1741 gagtttgttc acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt
     1801 cttggaattt gcccctttga gtttggagcg aggctaattc tcaagcctct tagcggttca
     1861 aaggtatttt ctaaacccgt ttccaggtgt tgtgaaagcc accgctaatt caaagcaatc
     1921 tagcgtttaa acttaagctt ggtaccgaat taattctgca gatatcaaca agtttgtaca
     1981 aaaaagcagg ctggcgccac catggacaag aagtactcca ttgggctcgc tatcggtacc
     2041 aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa
     2101 gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggagc cctcctgttc
     2161 gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc
     2221 cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg
     2281 gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac
     2341 gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca
     2401 accatatatc atctgaggaa gaagctggta gacagtactg ataaggctga cttgcggttg
     2461 atctatctcg cgctggcgca catgatcaaa tttcggggac acttcctcat cgagggggac
     2521 ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat
     2581 cagcttttcg aggagaaccc gatcaacgca tccggcgttg acgccaaagc aatcctgagc
     2641 gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag
     2701 aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc caactttaaa
     2761 tctaacttcg acctggccga agatgccaag ctgcaactga gcaaagacac ctacgatgat
     2821 gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt tttggcggca
     2881 aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac ggagatcacc
     2941 aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca agacttgact
     3001 ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat tttcttcgat
     3061 cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga ggaattttac
     3121 aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct ggtaaagctg
     3181 aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat cccccaccag
     3241 attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta cccctttttg
     3301 aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta ctatgtaggc
     3361 cccctcgctc ggggaaattc cagattcgcg tggatgactc gcaaatcaga agagaccatc
     3421 actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc cttcatcgaa
     3481 aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa acactctctg
     3541 ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt cacagaaggg
     3601 atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga cctcctcttc
     3661 aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa aaagattgaa
     3721 tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc cctgggaacg
     3781 tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga ggagaacgag
     3841 gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga gatgattgaa
     3901 gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca gctcaagaga
     3961 cgccgatata caggatgggg gcggctgtca agaaaactga tcaatggcat ccgagacaag
     4021 cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa ccggaacttc
     4081 atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa agcacaagtt
     4141 tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag cccagctatc
     4201 aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt aatgggaagg
     4261 cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac ccagaaggga
     4321 cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga actggggtcc
     4381 caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa gctctacctg
     4441 tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat caaccggttg
     4501 tccgactacg acgtggatgc tatcgtgccc caaagctttc tcaaagatga ttctattgat
     4561 aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt cccctcagaa
     4621 gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa actgatcaca
     4681 caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga gttggataaa
     4741 gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca cgtggcccaa
     4801 attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat tcgagaggtg
     4861 aaagttatta ctctgaagtc taagctggtc tcagatttca gaaaggactt tcagttttat
     4921 aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa tgcagtggta
     4981 ggcactgcac ttatcaaaaa atatcccaag ctggaatctg aatttgttta cggagactat
     5041 aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg caaggccacc
     5101 gctaagtact tcttttacag caatattatg aattttttca agaccgagat tacactggcc
     5161 aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg agaaatcgtg
     5221 tgggacaagg gtagggattt cgcgacagtc cgcaaggtcc tgtccatgcc gcaggtgaac
     5281 atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat cctcccgaaa
     5341 aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa atacggcgga
     5401 ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga gaaagggaag
     5461 tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga gcgatccagc
     5521 ttcgagaaaa accccatcga ctttctcgaa gcgaaaggat ataaagaggt caaaaaagac
     5581 ctcatcatta agctgcccaa gtactctctc tttgagcttg aaaacggccg gaaacgaatg
     5641 ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc taaatacgtt
     5701 aatttcttgt atctggccag ccactatgaa aagctcaaag ggtctcccga agataatgag
     5761 cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat cgagcaaata
     5821 agcgagttct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt gctttctgct
     5881 tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat ccacttgttt
     5941 actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac catagacaga
     6001 aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca gtcaattacg
     6061 gggctctatg aaacaagaat cgacctctct cagctcggtg gagacggcac cggcgggccc
     6121 aagaagaaga ggaaggtata cccatacgat gttcctgact atgcgggcta tccctatgac
     6181 gtcccggact atgcaggatc gtatccttat gacgttccag attacgctgg atctggatct
     6241 ggcgccacca acttcagcct gctgaagcag gcaggcgacg tggaagagaa ccctggccct
     6301 ggatctctcg acgcgatcgc acgtacggcc accatggctt caaactttac tcagttcgtg
     6361 ctcgtggaca atggtgggac aggggatgtg acagtggctc cttctaattt cgctaatggg
     6421 gtggcagagt ggatcagctc caactcacgg agccaggcct acaaggtgac atgcagcgtc
     6481 aggcagtcta gtgcccagaa gagaaagtat accatcaagg tggaggtccc caaagtggct
     6541 acccagacag tgggcggagt cgaactgcct gtcgccgctt ggaggtccta cctgaacatg
     6601 gagctcacta tcccaatttt cgctaccaat tctgactgtg aactcatcgt gaaggcaatg
     6661 caggggctcc tcaaagacgg taatcctatc ccttccgcca tcgccgctaa ctcaggtatc
     6721 tacagcgctg gaggaggtgg aagcggagga ggaggaagcg gaggaggagg tagcggacct
     6781 aagaaaaaga ggaaggtggc ggccgctgga tccccttcag ggcagatcag caaccaggcc
     6841 ctggctctgg cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct
     6901 atggtgcctc tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag
     6961 tcactgagcg ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct
     7021 ctgctgcacc tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc
     7081 gatcccggag tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg
     7141 aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa
     7201 gccattaccc ggctggtgac cggcagccag cggccccccg accccgctcc aactcccctg
     7261 ggaaccagcg gcctgcctaa tgggctgtcc ggagatgaag acttctcaag catcgctgat
     7321 atggacttta gtgccctgct gtcacagatt tcctctagtg ggcagggagg aggtggaagc
     7381 ggcttcagcg tggacaccag tgccctgctg gacctgttca gcccctcggt gaccgtgccc
     7441 gacatgagcc tgcctgacct tgacagcagc ctggccagta tccaagagct cctgtctccc
     7501 caggagcccc ccaggcctcc cgaggcagag aacagcagcc cggattcagg gaagcagctg
     7561 gtgcactaca cagcgcagcc gctgttcctg ctggaccccg gctccgtgga caccgggagc
     7621 aacgacctgc cggtgctgtt tgagctggga gagggctcct acttctccga aggggacggc
     7681 ttcgccgagg accccaccat ctccctgctg acaggctcgg agcctcccaa agccaaggac
     7741 cccactgtct ccgctgcgat cgctgtcgag ggatctggcg ccaccaactt cagcctgctg
     7801 aagcaggcag gcgacgtgga agagaaccct ggccctggat ctaaagactt tcccaaaaag
     7861 aaaagaaaag tgggtagtat gaaatcaatt cgctgtaaaa actgcaacaa actgttattt
     7921 aaggcggata gttttgatca cattgaaatc aggtgtccgc gttgcaaacg tcacatcata
     7981 atgctgaatg cctgcgagca tcccacggag aaacattgtg ggaaaagaga aaaaatcacg
     8041 cattctgacg aaaccgtgcg ttatggctcc actagtgggc acaagcttaa tggcggtggc
     8101 ggagggatgg atgctaagtc actaactgcc tggtcccgga cactggtgac cttcaaggat
     8161 gtatttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca gcagatcgtg
     8221 tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta tcagcttact
     8281 aagccagatg tgatcctccg gttggagaag ggagaagagc cctagaacaa ttctaaggcc
     8341 ttcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8401 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8461 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8521 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8581 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8641 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8701 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     8761 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8821 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8881 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gtcagtatca agcttgtcga
     8941 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     9001 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     9061 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9121 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9181 tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagcgg ccgcataact
     9241 tcgtataatg tatgctatac gaagttatct gcagtctcga caagcttgtt ctagagcatg
     9301 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgc attaatgaat
     9361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     9781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11341 cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc
    11401 gcatagttaa
//
