LOCUS       Exported                5891 bp ds-DNA     circular SYN 13-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   NKI-AVL
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2018 from SnapGene 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    4..131
                     /label=pURA3 [*]
                     /note="-350 -> -100"
     misc_feature    133..253
                     /label=pURA3 [*]
                     /note="-350 -> -100"
     primer_bind     254..271
                     /label=M13-fwd
     misc_feature    272..277
                     /label=SphI
     misc_feature    284..289
                     /label=AatII
     primer_bind     complement(296..316)
                     /label=M13-rev
     misc_recomb     complement(317..350)
                     /label=LoxP
     misc_feature    351..382
                     /label=pTEF-CaURA3-tTEF [*]
     misc_feature    384..552
                     /label=pTEF-CaURA3-tTEF [*]
     misc_feature    552
                     /label=pTEF-CaURA3-tTEF [*] [*] [5']
     misc_feature    553..554
                     /label=mutations
     misc_feature    555..747
                     /label=pTEF-CaURA3-tTEF [*] [*] [3']
     misc_feature    748
                     /label=mutation
     misc_feature    749..1769
                     /label=pTEF-CaURA3-tTEF [*] [*] [*]
     misc_recomb     complement(1770..1803)
                     /label=LoxP
     misc_feature    1802..2499
                     /label=#215_RPL15Ap-gBlock
     primer_bind     1804..1823
                     /label=T7
     primer_bind     1804..1823
                     /label=#132_T7
     promoter        1837..2459
                     /label=rpl15Apromoter
     misc_feature    2460..2906
                     /label=#181_NLS_PP7
     CDS             2466..2864
                     /label=PP7-1xGFP
     misc_feature    2466..2830
                     /label=PP7 PS
     misc_feature    2844..2864
                     /label=NLS
                     /note="NLS"
                     2877..3594
                     /note="note=""Fragment extracted from 1109/1110 PCR.xdna 
                     [719 nt] : (HindIII[7] / BamHI[730])"
     misc_feature    2877..3593
                     /label=ENVY ORF
     misc_feature    3608..3861
                     /label=Cyc1 Terminator
                     /note="note=""Fragment extracted from pED45.xdna [254 nt] :
                     (XhoI[2020] / KpnI[2278])"
     primer_bind     complement(3877..3896)
                     /label=T3
     misc_feature    3878..3897
                     /label=T3
                     /note="note=""direct strand 
                     translation""translation=""PFSEG*"""
     misc_feature    3903..3978
                     /label=tURA3 [*]
     misc_feature    3980..4038
                     /label=tURA3 [*] [*]
     misc_feature    4040..4152
                     /label=tURA3 [*]
     rep_origin      complement(4167..4280)
                     /direction=LEFT
                     /label=ColE1 origin [*]
     misc_feature    4281
                     /label=mutation?
     rep_origin      complement(4282..4379)
                     /direction=LEFT
                     /label=ColE1 origin [*]
     misc_feature    4380..4381
                     /label=mutations?
     rep_origin      complement(4382..4625)
                     /direction=LEFT
                     /label=ColE1 origin [*] [*]
     misc_feature    4626
                     /label=mutation?
     rep_origin      complement(4627..4795)
                     /direction=LEFT
                     /label=ColE1 origin [*] [*] [*]
     CDS             complement(4947..5606)
                     /label=AmpR
     CDS             complement(4947..5606)
                     /label=beta Lactamase
                     /note="dnas_title=""Beta Lactamase"" - codon_start=True - 
                     label=Beta Lactamase - SerialCloner_Color=""&h8FBC8F"" - 
                     SerialCloner_Show=""True"" - SerialCloner_Protect=""False""
                     - SerialCloner_Arrow=""True"" - note=""Copied from "" - 
                     label=beta Lactamase - translation=""MSTFKVL"
     promoter        complement(5846..5874)
                     /label=Copied from _Amp prom
                     /note="label=Amp prom - SerialCloner_Color=""&h4682B4"" - 
                     SerialCloner_Show=""True"" - SerialCloner_Protect=""False""
                     - SerialCloner_Arrow=""True"" - note=""Copied from "" - 
                     complement(6385..6413)"
     promoter        complement(5846..5874)
                     /label=Amp prom
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcgta tcggacgtct cccttcatgg
      301 tcatagctgt ttcctgataa cttcgtataa tgtatgctat acgaagttat tagcttgcct
      361 cgtccccgcc gggtcacccg gcgagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgacagtca acactaatac ctatagtgag agagcagaaa ctcatgcctc
      781 accagtagca cagcgattat ttcgattaat ggaactgaag aaaaccaatt tatgtgcatc
      841 aattgacgtt gataccacta aggagttcct tgaattaatt gataaattag gtccttatgt
      901 atgcttaatc aagactcata ttgatataat caatgatttt tcctatgaat ccactattga
      961 accattatta gaactttcac gtaaacatca atttatgatt tttgaagata gaaaatttgc
     1021 tgatattggt aataccgtaa agaaacaata tattggtgga gtttataaaa ttagtagttg
     1081 ggcagatatt accaatgctc atggtgtcac tgggaatgga gtggttgaag gattaaaaca
     1141 gggagctaaa gaaaccacca ccaaccaaga gccaagaggg ttattgatgt tagctgaatt
     1201 atcatcagtg ggatcattag catacggaga atattctcaa aaaactgttg aaattgctaa
     1261 atccgataag gaatttgtta ttggatttat tgcccaacgt gatatgggtg gccaagaaga
     1321 aggatttgat tggcttatta tgacacctgg agttggatta gatgataaag gtgatggatt
     1381 aggacaacaa tatagaactg ttgatgaagt tgttagcact ggaactgata ttatcattgt
     1441 tggtagagga ttgtttggta aaggaagaga tccagatatt gaaggtaaaa ggtatagaaa
     1501 tgctggttgg aatgcttatt tgaaaaagac tggccaatta taatcagtac tgacaataaa
     1561 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     1621 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     1681 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     1741 atactgctgt cgattcgata ctaacgccga taacttcgta taatgtatgc tatacgaagt
     1801 tattaatacg actcactata gggcgaattg gagctcgtat tcaagatatt cttatcaaag
     1861 cacttatttt ttcattatat ttctatattg atttaaggac ggtacgctat tttctttgta
     1921 ccttttacta tgcataaaaa aaggggaagt gtttaacaat cgacacttac ttaatatgtt
     1981 ttgccgccct tcataagagg gtgtttctaa aatttattgg gcaagaatga gatggactcg
     2041 caccctacat gacgtttaaa tatttagtgt taaggttcag aacatgcacc aggtgcgaca
     2101 tgtgttgcga ttatcatgac aatgtctcta tccgagatgc atttgtagta tcaattgatg
     2161 cgtattatga catgatttac atagcataca tcgtcaaaca tgatattata ttcttttttt
     2221 gataaatgta cggatttaaa gctgtcgaat atattttctg aaatttcttg gagctgacgc
     2281 aaaattttca aaggtgctaa aatttttcaa gatttctcac ttttgcttgg taacaaagaa
     2341 tgatggcatt gcatttttac caccggtaca tttaactgct atttctcacg tttctttccc
     2401 tatccttaag taattctttt acaatctaag aaaaccacga tcaaacaaat aaatcagcat
     2461 ctagaatgtc caaaaccatc gttctttcgg tcggcgaggc tactcgcact ctgactgaga
     2521 tccagtccac cgcagaccgt cagatcttcg aagagaaggt cgggcctctg gtgggtcggc
     2581 tgcgcctcac ggcttcgctc cgtcaaaacg gagccaagac cgcgtatcgc gtcaacctaa
     2641 aactggatca ggcggacgtc gttgattccg gacttccgaa agtgcgctac actcaggtat
     2701 ggtcgcacga cgtgacaatc gttgcgaata gcaccgaggc ctcgcgcaaa tcgttgtacg
     2761 atttgaccaa gtccctcgtc gcgacctcgc aggtcgaaga tcttgtcgtc aaccttgtgc
     2821 cgctgggccg tggatccccc gggccaaaaa agaagagaaa ggtagaattc gatatcatgt
     2881 ctaaaggcga ggaattgttt acaggtgtag ttcctatttt ggtcgaacta gatggagatg
     2941 tcaacggtca taagttttct gtacgtggtg aaggcgaagg tgatgctact aatggcaaac
     3001 tgactctgaa gttcatctgt acgaccggaa aattgccagt tccatggcct acattagtaa
     3061 caacattatg ttacggagtt caatgctttg ctagataccc agatcacatg aagcagcatg
     3121 actttttcaa aagtgctatg ccagaaggtt atgtgcagga gagaactatc tccttcaaag
     3181 atgatggtac ttataagaca agagcagagg taaagtttga aggcgataca ttggttaata
     3241 gaattgagct taaaggcatt gattttaagg aagatgggaa tatcctcgga cataaacttg
     3301 aatacaattt caattcacat aatgtttata tcacagcaga taaacaaaag aatggtataa
     3361 aagcgaactt taagacaaga cataacgtgg aagatggtag cgtccaatta gccgaccatt
     3421 accaacaaaa cactcctatc ggggacgggc cagttctcct tccagacaac cactacctat
     3481 caacccaatc tgttctatct aaagacccta atgaaaaacg agatcacatg gtcttgttag
     3541 agttcgtgac tgccgctggt ataacattag gaatggacga attgtacaaa taaggatcct
     3601 aactcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
     3661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
     3721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
     3781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
     3841 gaaggcttta atttgcggcc ggtacccagc ttttgttccc tttagtgagg gttaatttcc
     3901 tgaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     3961 aattatatca gttattacgc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa
     4021 aaaaaattgg aaagaaaatg cttcatggcc tttataaaaa ggaactatcc aatacctcgc
     4081 cagaaccaag taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa
     4141 agatggacgc atttaattaa ttcgaaggcc gcgttgctgg cgtttttcca taggctcggc
     4201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4321 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4381 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4441 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4501 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4561 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4621 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4681 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4741 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4801 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4861 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4921 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4981 atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat aactacgata
     5041 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5101 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5161 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5221 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5281 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5341 tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5401 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5461 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5521 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5581 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5641 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5701 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5761 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5821 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaagtatcg
     5881 ttcgaattaa t
//
