LOCUS       pRC0878_(2)             2556 bp ds-DNA     circular     08-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    706..710
                     /label="EcoRV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    707..710
                     /label="BsaI TATC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    711..789
                     /label="Domain I"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     IRES            711..1376
                     /label="synIRES-RC25 (iHRVB3-based)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    790..810
                     /label="domain1-2 junction"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    825..901
                     /label="Domain II"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    904..948
                     /label="Domain III"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    956..1197
                     /label="Domain IV"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1035..1071
                     /label="conserved"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1102..1141
                     /label="Apt17"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1200..1224
                     /label="conserved"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1205..1313
                     /label="Domain V"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1293..1310
                     /label="conserved"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1341..1376
                     /label="Domain VI?"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      complement(1396..1492)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#ff6666
                     /ApEinfo_fwdcolor=#ff6666
     CDS             complement(1493..2350)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     primer_bind     2113..2132
                     /label="Amp-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(2351..2481)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#ff6666
                     /ApEinfo_fwdcolor=#ff6666
     origin          complement(2482..689)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctGTG GATAACCGTG CGGCCGCtcg gTCTCaTATC ttaaaacagc
      721 ggatgggtac cccaccatcc gacccactgg gtgtagtact ctggtacttc gtacctttgt
      781 acgcctgttc ttcccattgt acccttcctg aacttccaac ccaagtaacg ttagaagctc
      841 aacatttagt acaacaggaa gcaccacatc cagtggtgtt tagtacaagc acttctgttt
      901 ccccggagcg aggtataggc tgtacccact gccaaaaacc tttaaccgtt atccgccaac
      961 caactacgta aaagctagta gtattatgtt tttaactagg cgttcgatca ggtggatttc
     1021 ccctccacta gtttggtcga tgaggctagg aattccccac gggtgaccgt gtcctagcct
     1081 gcgtggcggc caacccagcC CACTCACTAT TTGTTTTCGC GCCCAGTTGC AAAAAGTGTC
     1141 GGGgctggga cgccttttta tagacatggt gtgaagactc gcatgtgctt ggttgtgatt
     1201 cctccggccc ctgaatgcgg ctaaccttaa ccctggagcc ttgtgtcaca aaccagtgat
     1261 gataaggtcg taatgagcaa ttccgggacg ggaccgacta ctttgggtgt ccgtgtttct
     1321 tatttttctt attattgtct tatggtcaca gcatatatat aacatatact gtgatcATGG
     1381 tGAGACCGCG GCCGCGATTA TCAaaaagga tcttcaccta gatcctttta aattaaaaat
     1441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1621 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2461 ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact
     2521 gagcgtcaga ccccgtagaa aagatcaaag gatctt
//