LOCUS       pDB2_0_Cry2_5__3        9305 bp    DNA        circular     24-FEB-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >pUC57
COMMENT     NcoI, SacI
COMMENT     Sequencing: pQE30 fw (1-1000);  2075rv (2050-1000); 1825 rv
            (3000-2000) ; EGFP C F (3100-4100); pCEPrev (3970-5000); CMV-F
            pCDNA (5100-6100)
COMMENT     
COMMENT     Nco test digest
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3456..3851
                     /locus_tag="BlasticidinR"
                     /label="BlasticidinR"
                     /ApEinfo_label="BlasticidinR"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4046..4083
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2192..2239
                     /locus_tag="Synth PolyA"
                     /label="Synth PolyA"
                     /ApEinfo_label="Synth PolyA"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2267..2609
                     /locus_tag="SV40 ori+pro"
                     /label="SV40 ori+pro"
                     /ApEinfo_label="SV40 ori+pro"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1881..1900
                     /note="Seq. Halo Tev #2 fw"
                     /locus_tag="Seq. Halo Tev #2 fw"
                     /label="Seq. Halo Tev #2 fw"
                     /ApEinfo_label="Seq. Halo Tev #2 fw"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2409..2605
                     /locus_tag="SV40 early promoter"
                     /label="SV40 early promoter"
                     /ApEinfo_label="SV40 early promoter"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3384..3449
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2154..2191
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6787..7065
                     /locus_tag="PolyA"
                     /label="PolyA"
                     /ApEinfo_label="PolyA"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5198..5400
                     /locus_tag="hCMV promoter"
                     /label="hCMV promoter"
                     /ApEinfo_label="hCMV promoter"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5114..5197
                     /locus_tag="hCMV minEnh"
                     /label="hCMV minEnh"
                     /ApEinfo_label="hCMV minEnh"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3863..3993
                     /locus_tag="SV40 PolyA signal"
                     /label="SV40 PolyA signal"
                     /ApEinfo_label="SV40 PolyA signal"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2662..3377
                     /locus_tag="CFP"
                     /label="CFP"
                     /ApEinfo_label="CFP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3447..3449
                     /locus_tag="PuroR"
                     /label="PuroR"
                     /ApEinfo_label="PuroR"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3998..4045
                     /locus_tag="Frt F3"
                     /label="Frt F3"
                     /ApEinfo_label="Frt F3"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7080..7100)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(7468..8150)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(7106..7128)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2154..2187
                     /locus_tag="LoxP(2)"
                     /label="LoxP(2)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     4046..4079
                     /locus_tag="LoxP(3)"
                     /label="LoxP(3)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8248..8907)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2669..2686)
                     /locus_tag="1927"
                     /label="1927"
                     /ApEinfo_label="1927"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3255..3276
                     /locus_tag="1034"
                     /label="1034"
                     /ApEinfo_label="1034"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3313..3332
                     /locus_tag="814"
                     /label="814"
                     /ApEinfo_label="814"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3313..3332
                     /locus_tag="838"
                     /label="838"
                     /ApEinfo_label="838"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3287..3305
                     /locus_tag="729"
                     /label="729"
                     /ApEinfo_label="729"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2892..2911
                     /locus_tag="734"
                     /label="734"
                     /ApEinfo_label="734"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7084..7100)
                     /locus_tag="43"
                     /label="43"
                     /ApEinfo_label="43"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2431..2448
                     /locus_tag="811"
                     /label="811"
                     /ApEinfo_label="811"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3023..3042)
                     /locus_tag="1925"
                     /label="1925"
                     /ApEinfo_label="1925"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3251..3270
                     /locus_tag="1024"
                     /label="1024"
                     /ApEinfo_label="1024"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     9284..9302
                     /locus_tag="805"
                     /label="805"
                     /ApEinfo_label="805"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3282..3304
                     /locus_tag="1584"
                     /label="1584"
                     /ApEinfo_label="1584"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3313..3332)
                     /locus_tag="1023"
                     /label="1023"
                     /ApEinfo_label="1023"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3345..3367
                     /locus_tag="870"
                     /label="870"
                     /ApEinfo_label="870"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2968..2987)
                     /locus_tag="735"
                     /label="735"
                     /ApEinfo_label="735"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3244..3263)
                     /locus_tag="1825"
                     /label="1825"
                     /ApEinfo_label="1825"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3951..3971)
                     /locus_tag="792"
                     /label="792"
                     /ApEinfo_label="792"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7112..7131)
                     /locus_tag="1069"
                     /label="1069"
                     /ApEinfo_label="1069"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2312..2331)
                     /locus_tag="2052"
                     /label="2052"
                     /ApEinfo_label="2052"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2268..2288)
                     /locus_tag="2075"
                     /label="2075"
                     /ApEinfo_label="2075"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(5127..5147)
                     /locus_tag="2076"
                     /label="2076"
                     /ApEinfo_label="2076"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7096..7119)
                     /locus_tag="30nt_M13R"
                     /label="30nt_M13R"
                     /ApEinfo_label="30nt_M13R"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5308..5327
                     /locus_tag="CMVF_pCDNA3"
                     /label="CMVF_pCDNA3"
                     /ApEinfo_label="CMVF_pCDNA3"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3314..3335
                     /locus_tag="EGFP C F"
                     /label="EGFP C F"
                     /ApEinfo_label="EGFP C F"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2706..2727)
                     /locus_tag="EGFP Nrev"
                     /label="EGFP Nrev"
                     /ApEinfo_label="EGFP Nrev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7083..7100)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5396..5416
                     /locus_tag="pCMV forward"
                     /label="pCMV forward"
                     /ApEinfo_label="pCMV forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(29..51)
                     /locus_tag="PGEX 3'"
                     /label="PGEX 3'"
                     /ApEinfo_label="PGEX 3'"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5495..6769
                     /locus_tag="trunc hCD4"
                     /label="trunc hCD4"
                     /ApEinfo_label="trunc hCD4"
                     /ApEinfo_fwdcolor="#ffa579"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     9219..9236
                     /locus_tag="PQE30 forward"
                     /label="PQE30 forward"
                     /ApEinfo_label="PQE30 forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     5395..5414
                     /locus_tag="pCEP-Forward"
                     /label="pCEP-Forward"
                     /ApEinfo_label="pCEP-Forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3954..3973
                     /locus_tag="pCEP-Reverse"
                     /label="pCEP-Reverse"
                     /ApEinfo_label="pCEP-Reverse"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2079..2143
                     /locus_tag="FLAG3x"
                     /label="FLAG3x"
                     /ApEinfo_label="FLAG3x"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1128..1169
                     /locus_tag="GSG-Linker"
                     /label="GSG-Linker"
                     /ApEinfo_label="GSG-Linker"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            1185..2071
                     /note="Halo-Tag"
                     /locus_tag="Halo-Tag"
                     /label="Halo-Tag"
                     /ApEinfo_label="Halo-Tag"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1923..1942
                     /note="Seq. Halo Tev #1 fw"
                     /locus_tag="Seq. Halo Tev #1 fw"
                     /label="Seq. Halo Tev #1 fw"
                     /ApEinfo_label="Seq. Halo Tev #1 fw"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta agGTTTAAAC aggaaccgaa gggaggtcag tgtagcggca
      241 tgtcccagtg aggagctgga atgagatgag gacaagatac agcatgagca gcatgtggca
      301 ggcctgatgt ccgagacgag gactaggatt cagccttcag tgcactgaag gatggtcagt
      361 agggcagggc atgattggac ttaggtctga aaaagctccc tggcctgctg ggtggagatt
      421 ggacagtgta ggccggagcg ggcacaggga ggccagagag gaggcagcac agacagggtg
      481 gtggccgcaa ggagaaagac tggacagatt cgctgcattc ggagcctctt cccttcttgg
      541 ggatgaagat gagtaacatg agatgggaag aacacgggtt tttctagttg gcccagctag
      601 gctgcctcca tgggcagcat gactcctcca caggggccct gctctttgct gcttggggag
      661 gctgattcag ggttgcagag ggaatgcctc accttcaaag caggacagca ggcaggccaa
      721 atcagtctct ggccacagcc catgccattt ggggcccaaa agctggtcct gctaccagca
      781 ggcctgcttg gtcatgtcct ccttggacat ttccaggcaa agtcactgct gaaagcaagg
      841 ccgaggggca ggtctgagga gcagcatgct ggcattccat tgataagtgt gctccctgtc
      901 ccctaaagag ctgctgctgc atagtgtggg atcattttgg ctgcatgcac agtctgtgtg
      961 accctttgac acgcttccct acagGCCCAA GACCACTACC CAGTGGCCCA GCATCCCCCA
     1021 AACGCAAGCT GGAAGCAGCC GAGGAACCAC CTGGTGAAGA ACTCAGCAAA CGGGCCCGGG
     1081 TGGCAGAGTT GCCAACCCCA GAGCTGCCGA GCAAGGATGC CACCGGTGGC GGTGGTAGCG
     1141 GCGGTGGTGG CTCTGGTGGC GGTGGTTCAA CGCGTGGTGG CAGCGCAGAA ATCGGTACTG
     1201 GCTTTCCATT CGACCCCCAT TATGTGGAAG TCCTGGGCGA GCGCATGCAC TACGTCGATG
     1261 TTGGTCCGCG CGATGGCACC CCTGTGCTGT TCCTGCACGG TAACCCGACC TCCTCCTACG
     1321 TGTGGCGCAA CATCATCCCG CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA
     1381 TCGGTATGGG CAAATCCGAC AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT
     1441 TCATGGATGC CTTCATCGAA GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC ATTCACGACT
     1501 GGGGCTCCGC TCTGGGTTTC CACTGGGCCA AGCGCAATCC AGAGCGCGTC AAAGGTATTG
     1561 CATTTATGGA GTTCATCCGC CCTATCCCGA CCTGGGACGA ATGGCCAGAA TTTGCCCGCG
     1621 AGACCTTCCA GGCCTTCCGC ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG
     1681 TTTTTATCGA GGGTACGCTG CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG
     1741 ACCATTACCG CGAGCCGTTC CTGAATCCTG TTGACCGCGA GCCACTGTGG CGCTTCCCAA
     1801 ACGAGCTGCC AATCGCCGGT GAGCCAGCGA ACATCGTCGC GCTGGTCGAA GAATACATGG
     1861 ACTGGCTGCA CCAGTCCCCT GTCCCGAAGC TGCTGTTCTG GGGCACCCCA GGCGTTCTGA
     1921 TCCCACCGGC CGAAGCCGCT CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA
     1981 TCGGCCCGGG TCTGAATCTG CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG
     2041 CGCGCTGGCT GTCGACGCTC GAGATTTCCG GTactagtGA CTACAAAGAC CATGACGGTG
     2101 ATTATAAAGA TCATGACATC GATTACAAGG ATGACGATGA CAAGTTAGAT CTaATAACTT
     2161 CGTATAGCAT ACATTATACG AAGTTATTtt gAATAAAATA TCTTTATTTT CATTACATCT
     2221 GTGTGTTGGT TTTTTGTGTT GAGCTGATTT AACAAAAATT TAACGCTGTG GAATGTGTGT
     2281 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGGCAGGC AGAAGTATGC AAAGCATGCA
     2341 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT
     2401 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC
     2461 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT
     2521 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT
     2581 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT ATTGTATATC CATTTTCGGA TCTGATCAGc
     2641 acTGAGCAgg taccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2701 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2761 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2821 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc
     2881 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2941 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3001 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3061 acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca
     3121 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     3181 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3241 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
     3301 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     3361 tggacgagct gtacaaggaa ttcggaagcg gagctactaa cttcagcctg ctgaagcagg
     3421 ctggagacgt ggaggagaac cctggacctc acgtgGCCAA GCCTTTGTCT CAAGAAGAAT
     3481 CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT GAAGACTACA
     3541 GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC AATGTATATC
     3601 ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT GCTGCGGCAG
     3661 CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC TTGAGCCCCT
     3721 GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCC ATAGTGAAGG
     3781 ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT GGTTATGTGT
     3841 GGGAGGGCTA ActcgagACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
     3901 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
     3961 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATAGAA GTTCCTATTC cGAAGTTCCT
     4021 ATTCttcaaa taGtATAGGA ACTTCATAAC TTCGTATAGC ATACATTATA CGAAGTTATt
     4081 tgaTTAATTA AGCGATCGCG GATGCCTGAG Agtgagtgac agcagcctag attcaacctc
     4141 aCgaaCgaag ttgggagtgg gggggcctac tgccctgcca gctgcaggtt gaCacatagc
     4201 aCactagatg aaaatctggt gggaccaccc aatgctcagc cactaaagct ttaccatttt
     4261 acagtggcag ccgggttcca tcctggctta gggaatgagt actttctggt tgatatctgt
     4321 gtgaccttta ccattgggcc ggctatggtg gctcatgtat gtaatcccag cactttggga
     4381 ggccaaggtg ggaggatcgc ttgaggccag gagtacaaga ccaacctggc caaaccaaca
     4441 tagtgagact ccatctctaa aaacataata ataataataa taataatctg gtgggactgc
     4501 atgactttta ggatttcagc tcaaaaatcc caccattctg acttttatca gagatttaaa
     4561 agtcaagccc ttttgtattt ttttgccagc taagggaaaa agggaggcat gggggctgag
     4621 gggtggcgct ttctacctat taaaatgagt cacactcaag agaaagaaag ctgagacctg
     4681 cgtgtgaagc agcggggaaa aggcgcattg cagggtctga ggctgctcca ggccccctgc
     4741 tttccgacag gctgtgttct ataggccagc cagtggaggg cagcactgac cttgagaagc
     4801 ctggacagag ggctgtgctg tgtcttactt ggcccagccc tgcctgagga ccagtagacc
     4861 cagtctgtca tcatctttgc cttttgcggc ctctcctccc ttctcaccca tcctgtggcc
     4921 tcagctctgc acctccctgg cagcgctcat cttggaagcc tcctgaatgt catcaaaaca
     4981 ctggcttctc ctttcccaag ggaggaaggg ccttttatgc cactcttggg gaaatgtaaa
     5041 ttcctgtccc ctctgagtca tcatctcaag ctgttcctta tgcaccatgt cttcgtcaCC
     5101 TGCAGGTCAA TTCCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
     5161 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC
     5221 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC
     5281 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC
     5341 GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT
     5401 CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA CCTCCATAGA
     5461 AGACACCGGG ACCGATCCAG CCTGGGGATC CACCATGAAC CGGGGAGTCC CTTTTAGGCA
     5521 CTTGCTTCTG GTGCTGCAAC TGGCGCTCCT CCCAGCAGCC ACTCAGGGAA AGAAAGTGGT
     5581 GCTGGGCAAA AAAGGGGATA CAGTGGAACT GACCTGTACA GCTTCCCAGA AGAAGAGCAT
     5641 ACAATTCCAC TGGAAAAACT CCAACCAGAT AAAGATTCTG GGAAATCAGG GCTCCTTCTT
     5701 AACTAAAGGT CCATCCAAGC TGAATGATCG CGCTGACTCA AGAAGAAGCC TTTGGGACCA
     5761 AGGAAACTTC CCCCTGATCA TCAAGAATCT TAAGATAGAA GACTCAGATA CTTACATCTG
     5821 TGAAGTGGAG GACCAGAAGG AGGAGGTGCA ATTGCTAGTG TTCGGATTGA CTGCCAACTC
     5881 TGACACCCAC CTGCTTCAGG GGCAGAGCCT GACCCTGACC TTGGAGAGCC CCCCTGGTAG
     5941 TAGCCCCTCA GTGCAATGTA GGAGTCCAAG GGGTAAAAAC ATACAGGGGG GGAAGACCCT
     6001 CTCCGTGTCT CAGCTGGAGC TCCAGGATAG TGGCACCTGG ACATGCACTG TCTTGCAGAA
     6061 CCAGAAGAAG GTGGAGTTCA AAATAGACAT CGTGGTGCTA GCTTTCCAGA AGGCCTCCAG
     6121 CATAGTCTAT AAGAAAGAGG GGGAACAGGT GGAGTTCTCC TTCCCACTCG CCTTTACAGT
     6181 TGAAAAGCTG ACGGGCAGTG GCGAGCTGTG GTGGCAGGCG GAGAGGGCTT CCTCCTCCAA
     6241 GTCTTGGATC ACCTTTGACC TGAAGAACAA GGAAGTGTCT GTAAAACGGG TTACCCAGGA
     6301 CCCTAAGCTC CAGATGGGCA AGAAGCTCCC GCTCCACCTC ACCCTGCCCC AGGCCTTGCC
     6361 TCAGTATGCT GGCTCTGGAA ACCTCACCCT GGCCCTTGAA GCGAAAACAG GAAAGTTGCA
     6421 TCAGGAAGTG AACCTGGTGG TGATGAGAGC CACTCAGCTC CAGAAAAATT TGACCTGTGA
     6481 GGTGTGGGGA CCCACCTCCC CTAAGCTGAT GCTGAGCTTG AAACTGGAGA ACAAGGAGGC
     6541 AAAGGTCTCG AAGCGGGAGA AGGCGGTGTG GGTGCTGAAC CCTGAGGCGG GGATGTGGCA
     6601 GTGTCTGCTG AGTGACTCGG GACAGGTCCT GCTGGAATCC AACATCAAGG TTCTGCCCAC
     6661 ATGGTCGACC CCGGTGCAGC CAATGGCCCT GATTGTGCTG GGGGGCGTCG CCGGCCTCCT
     6721 GCTTTTCATT GGGCTAGGCA TCTTCTTCTG TGTCAGGTGC CGGCACTGAA GGCGACCTGC
     6781 GGATCCCACG TCACTATTGT ATACTCTATA TTATACTCTA TGTTATACTC TGTAATCCTA
     6841 CTCAATAAAC GTGTCACGCC TGTGAAACCG TACTAAGTCT CCCGTGTCTT CTTATCACCA
     6901 TCAGGTGACA TCCTCGCCCA GGCTGTCAAT CATGCCGGTA TCGATTCCAG TAGCACCGGC
     6961 CCCACGCTGA CAACCCACTC TTGCAGCGTT AGCAGCGCCC CTCTTAACAA GCCGACCCCC
     7021 ACCAGCGTCG CGGTTACTAA CACTCCTCTC CCCGACCTGC AGCCCaagct tggcgtaatc
     7081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7921 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9241 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9301 tcgtc     
//
