LOCUS       Exported               11460 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 26, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        385..662
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     regulatory      1753..1762
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     exon            1759..2442
                     /label=GST
                     /note="GST"
                     /note="color: #00ff00; direction: RIGHT"
     CDS             2443..2463
                     /codon_start=1
                     /label=TEV cleavage site
                     /note="TEV cleavage site"
                     /note="color: #0000ff"
                     /translation="ENLYFQG"
     CDS             2470..7252
                     /codon_start=1
                     /label=hFIP200
                     /note="hFIP200"
                     /note="color: #0000ff"
                     /translation="MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVL
                     VVNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEE
                     SLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNS
                     YQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDS
                     LPEHEDSEKAEMKRSTELVLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRES
                     CQSTVHQQDETTIDTKDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRII
                     RPFIAECRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKR
                     AENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFV
                     MLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWA
                     GALVKDGKRLYEAEKSKRESFGKLFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPD
                     ISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDL
                     LSEQKASVSQTSPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDS
                     IDAHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLSSPNPISD
                     PQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNVQLERCRVVAQDSHFSI
                     QTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRNIIEKVKCSLEITLKEKHQKELLSLK
                     NEYEGKLDGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEK
                     DQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLK
                     ELEDTLQVRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQELKL
                     KVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQETENLRTEISKL
                     NQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEEESNILKAELNKVTSLHNQAF
                     EIEKNLKEQIIELQSKLDSELSALERQKDEKITQQEEKYEAIIQNLEKDRQKLVSSQEQ
                     DREQLIQKLNCEKDEAIQTALKEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDS
                     SSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRK
                     ENIINDLSDKLKSTMQQQEGSSGGSSGLSEDRARLLEEKKKLEEEVSKLRSSSFVPSPY
                     VATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQENIHMLSEEKQRIML
                     LERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVL
                     FTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLG
                     TKFYRVKAVSWNKKV"
     CDS             7258..8367
                     /codon_start=1
                     /label=MBP tag
                     /note="MBP tag"
                     /note="color: #0000ff"
                     /translation="ASMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDK
                     LEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNG
                     KLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPL
                     IAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNK
                     GETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKE
                     FLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMS
                     AFWYAVRTAVINAASGRQTVDEALKDAQTN"
     polyA_signal    8547..8602
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     primer_bind     complement(8963..8979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8987..9003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9011..9041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9011 .. 9017 / #ffffff / -10
                        2: 9018 .. 9035 / #ffffff
                        3: 9036 .. 9041 / #ffffff / -35"
     protein_bind    9056..9077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        9136..9331
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      9182..9317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     polyA_signal    9337..9471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(9710..10298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10469..11329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10469 .. 11260 / #ccffcc
                        2: 11261 .. 11329 / #ccffcc / signal sequence
                     Cleavage site after base 11260"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11330..11434)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgatc
     1741 gacggtatcg atgccaccat ggcatcccct atactaggtt attggaaaat taagggcctt
     1801 gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga gcatttgtat
     1861 gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt ggagtttccc
     1921 aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc catcatacgt
     1981 tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc agagatttca
     2041 atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc atatagtaaa
     2101 gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct gaaaatgttc
     2161 gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca tcctgacttc
     2221 atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct ggatgcgttc
     2281 ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga taagtacttg
     2341 aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt tggtggtggc
     2401 gaccatcctc caaaatcgga tgattacgat atcccaacga ccgaaaacct gtattttcag
     2461 ggtggtacca tgaagctcta tgtgtttctc gtgaataccg gcactaccct gacctttgat
     2521 accgaactga ccgtccagac cgtggctgac ctgaagcatg ctatccagtc taaatacaag
     2581 atcgcaattc agcaccaggt gctggtggtc aacggcggag agtgcatggc cgctgatcgc
     2641 agggtctgta cctattcagc cggcaccgac actaacccaa tcttcctctt taataaggag
     2701 atgattctgt gcgatagacc ccctgctatc cccaagacca cttttagcac tgagaacgac
     2761 atggaaatca aagtggagga atccctgatg atgccagccg tgttccacac cgtcgcttct
     2821 cgcactcagc tggcactcga gatgtacgaa gtggccaaga aactgtgctc tttctgtgag
     2881 ggtctcgtcc acgatgaaca tctgcagcac cagggctggg cagccattat ggctaacctg
     2941 gaggactgtt caaatagtta tcagaaactg ctcttcaagt ttgagtctat ctactcaaac
     3001 tatctgcaga gcatcgaaga cattaaactg aagctcacac acctgggaac cgcagtgagc
     3061 gtcatggcca agatccctct gctcgagtgc ctgactaggc attcctacag agaatgtctg
     3121 ggtaggctcg atagtctgcc agaacacgag gacagcgaga aagccgaaat gaagcgctct
     3181 acagagctgg tgctctcacc tgacatgcca aggacaacca acgaaagtct gctcaccagc
     3241 ttcccaaagt ccgtggagca tgtctccccc gacactgccg atgctgaatc tggaaaggag
     3301 attagagaaa gttgccagag caccgtgcac cagcaggacg agactacaat cgatactaag
     3361 gacggcgatc tgcccttctt taacgtcagt ctgctcgatt ggatcaacgt ccaggacaga
     3421 cctaatgatg tggagtccct ggtccggaag tgctttgata gcatgtcccg tctggaccca
     3481 cgtatcattc gtcctttcat cgcagagtgt cgtcagacca ttgccaagct ggacaaccag
     3541 aatatgaaag ctattaaggg tctggaagat cggctctacg ccctggacca gatgatcgct
     3601 agctgtggac gcctggtgaa cgagcagaag gaactcgctc agggtttcct ggcaaaccag
     3661 aaaagagccg agaatctcaa ggatgcatca gtgctgcctg acctgtgcct cagtcatgcc
     3721 aatcagctga tgattatgct ccagaaccac cgcaagctgc tcgacatcaa acagaagtgt
     3781 accactgcta aacaggagct ggcaaacaat ctccatgtga ggctgaagtg gtgctgtttc
     3841 gtcatgctgc acgcagacca ggatggcgag aagctccagg ccctgctcag gctggtgatt
     3901 gagctgctcg aaagagtgaa gatcgtcgag gctctgtcca ccgtcccaca gatgtactgc
     3961 ctggcagtgg tcgaggtggt cagacgtaaa atgtttatca agcactacag agaatgggca
     4021 ggtgccctcg tgaaagatgg caagcgtctg tatgaagccg agaaatctaa gcgtgagtca
     4081 ttcggcaaac tgtttcggaa gagcttcctc cgtaaccggc tgttccgcgg actcgacagt
     4141 tggccaccca gcttttgcac ccagaaacct aggaagttcg actgtgagct gccagatatc
     4201 tccctcaagg acctgcagtt tctccagtcc ttctgcccat ctgaggtgca gccctttctg
     4261 agggtccctc tgctctgtga cttcgaacct ctgcaccagc atgtgctggc cctccacaac
     4321 ctcgtcaagg ctgcacagag tctggatgag atgagccaga caatcaccga cctgctcagc
     4381 gaacagaagg cttctgtgtc acagacatcc ccacagtctg ctagctcccc ccgtatggag
     4441 agcaccgcag gcatcacaac cactacatcc ccacgtacac ctccaccact caccgtgcag
     4501 gatcccctgt gccctgctgt ctgtcctctg gaggaactct ctccagacag tattgatgca
     4561 cacactttcg actttgagac aatccctcat ccaaacatcg aacagaccat tcaccaggtg
     4621 tccctggacc tcgattctct ggccgagagc cccgaatccg attttatgtc cgctgtgaat
     4681 gagttcgtca ttgaggaaaa cctgtcttca cccaatccta tctcagaccc acagagtccc
     4741 gagatgatgg tggaatccct gtacagtagc gtcatcaacg ccattgattc tcggcgcatg
     4801 caggacacaa atgtgtgcgg caaggaggac tttggagatc atacctctct gaacgtccag
     4861 ctcgaacgct gtagggtggt cgctcaggat tctcatttct caatccagac tattaaggag
     4921 gacctgtgcc acttccgcac atttgtgcag aaggaacagt gcgacttcag taacagcctg
     4981 aaatgtacag ccgtggagat caggaacatc atcgaaaagg tcaagtgttc tctggagatc
     5041 accctcaaag aaaagcacca gaaagagctg ctctcactga agaacgaata cgagggaaaa
     5101 ctggacggtc tcattaagga aaccgaggaa aacgagaata aaatcaagaa actgaaggga
     5161 gagctggtgt gcctcgagga agtcctgcag aacaaggata atgaattcgc cctggtgaaa
     5221 catgaaaagg aggctgtcat ctgcctgcag aatgagaaag accagaagct gctcgaaatg
     5281 gagaacatta tgcactcaca gaattgtgag atcaaagaac tgaagcagag tagagagatc
     5341 gtgctggaag atctcaagaa actgcatgtc gagaatgacg aaaagctgca gctgctccgt
     5401 gccgaactcc agagcctgga gcagtcccac ctcaaggaac tggaggatac tctgcaggtg
     5461 cgccatatcc aggaattcga gaaggtgatg acagaccaca gagtcagcct cgaggaactg
     5521 aagaaagaga accagcagat cattaatcag atccaggagt cccacgctga aatcattcag
     5581 gaaaaagaga agcagctgca ggagctgaaa ctcaaggtgt ctgacctcag cgatacccgc
     5641 tgcaaactgg aagtcgagct ggccctcaag gaagctgaga ctgacgagat caaaattctg
     5701 ctcgaggaat ctcgggccca gcagaaagaa accctgaagt cactgctcga acaggagacc
     5761 gaaaatctcc gtactgagat cagcaaactg aaccagaaga ttcaggataa caatgagaat
     5821 taccaagtgg gactcgctga actgagaact ctcatgacaa tcgagaagga ccagtgtatc
     5881 tctgaactga tttcacgtca tgaggaagag tcaaacattc tgaaagcaga gctcaataaa
     5941 gtgactagtc tgcacaacca ggccttcgag atcgagaaga acctgaagga gcagatcatt
     6001 gaactgcagt ccaagctcga ttccgaactg tctgccctcg agaggcagaa agacgaaaag
     6061 atcacacagc aggaagagaa gtatgaggct atcattcaga acctcgaaaa agataggcag
     6121 aagctggtgt cctctcagga gcaggacaga gaacagctga ttcagaaact caattgcgag
     6181 aaggacgaag caatccagac agccctcaaa gagttcaagc tggagagaga agtggtcgag
     6241 aaagaactgc tcgagaaagt gaagcacctg gaaaaccaga tcgctaagtc acccgcaatt
     6301 gatagtaccc gtggtgactc aagtagcctg gtggccgagc tccaggaaaa gctgcaggaa
     6361 gagaaggcta agtttctgga gcagctcgaa gagcaggaaa aaagaaagaa cgaagagatg
     6421 cagaatgtgc gtacaagcct gatcgccgag cagcagacca acttcaatac cgtcctgact
     6481 cgtgagaaaa tgcggaagga aaacatcatt aatgacctca gcgataaact gaagtccacc
     6541 atgcagcagc aggagggctc atctggcggt tccagcggac tgtctgaaga cagagctcgt
     6601 ctgctcgaag agaagaaaaa gctggaagag gaagtcagta agctgaggtc ctcttcattc
     6661 gtgccaagcc cctacgtcgc tactgcaccc gagctgtatg gagcctgcgc tcctgagctg
     6721 cctggtgaat cagaccggag tgccgtggag actgctgatg aaggccgcgt cgactctgcc
     6781 atggagacaa gcatgatgtc cgtgcaggaa aacattcata tgctgtcaga ggaaaagcag
     6841 agaatcatgc tgctcgagcg taccctgcag ctcaaagagg aagagaacaa gcgcctcaat
     6901 cagaggctga tgtcacagag tatgagtagc gtgtcctctc gccactccga gaagatcgcc
     6961 attagggatt tccaagtggg agacctggtc ctcatcattc tggacgaacg ccatgataac
     7021 tacgtgctgt ttactgtctc tcctacactg tatttcctcc acagcgagtc cctgcccgct
     7081 ctggacctca agcctggtga aggcgcatct ggcgcctcaa ggagaccctg ggtgctggga
     7141 aaagtcatgg agaaggaata ctgccaggct aaaaaggcac agaaccgttt taaggtgcct
     7201 ctgggcacca aattctatcg ggtgaaggcc gtctcctgga ataaaaaggt ccccggggct
     7261 agcatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa aggctataac
     7321 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
     7381 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
     7441 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
     7501 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
     7561 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
     7621 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
     7681 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
     7741 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
     7801 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
     7861 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
     7921 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
     7981 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
     8041 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag
     8101 ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg
     8161 ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc
     8221 gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct
     8281 ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat
     8341 gaagccctga aagacgcgca gactaatccc gggtgatatc ttaattaatt aactcgagga
     8401 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     8461 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     8521 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     8581 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     8641 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     8701 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     8761 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     8821 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     8881 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     8941 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9121 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9301 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     9361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     9481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9901 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
