LOCUS       mCrimsonT3_01              6892 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     intron          complement(4049..5065)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="addgene"
                     /identity="99.902"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             5862..6722
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3217..3883)
                     /note="pLannotate"
                     /label="CyRFP1"
                     /database="addgene"
                     /identity="96.102"
                     /match_length="95.01424501424502"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1321..1920)
                     /note="pLannotate"
                     /label="PuroR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(2274..2702)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5346..5725)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(1931..2260)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(5066..5344)
                     /note="pLannotate"
                     /label="chicken beta-actin promoter"
                     /database="addgene"
                     /identity="98.582"
                     /match_length="100.35587188612101"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(2748..2972)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
     polyA_signal    1031..1161
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="addgene"
                     /identity="98.496"
                     /match_length="97.08029197080292"
                     /fragment="False"
                     /other="polyA signal"
     promoter        5757..5861
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2723..2744)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="6.197183098591549"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(3161..3183)
                     /note="pLannotate"
                     /label="Clover"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.221288515406162"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
     1381 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc
     1441 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct
     1501 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg
     1561 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga
     1621 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct
     1681 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat
     1741 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg
     1801 gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg
     1861 tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat
     1921 gcgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
     1981 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     2041 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     2101 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     2161 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     2221 ctggggagcc tggggacttt ccacacccta actgacacac attccacaga attaattcgc
     2281 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2341 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2401 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     2461 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2521 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2581 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2641 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2701 gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccac
     2761 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
     2821 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
     2881 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
     2941 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg
     3001 ccctctagac tcgagcggcc gccactgtgc tggatatctg cagaattcca ccacactgga
     3061 ctagtggatc cgagctcggt accaagcttc attacatgat cacgcactta gtcttggact
     3121 tcttcttttt cttcttgccg tccttgctca tcttcttaag cttgtacagc tcgtccatgc
     3181 cattaagttt gtgccccagt ttgctaggga ggtcgcagta tctggccaca gccacctcgt
     3241 gctgctcgac gtaggtctcg ttgtcggcct ccttgattct ttccagtctg cggtccacgt
     3301 agtggacgcc gggcatcttg acgggtttct tggatttgta tgtggtcttg aagttgcagt
     3361 gcaggtggcc cccgcccacg agcttcaggg ccatgtcgca tctgccttcc agggcgccgt
     3421 cagcggggta cagggtctcg gtgctgggct cccagcccag tgttgtctgc ttcatcacgg
     3481 ggccgttggc tgggaagttc acccctctca gcttcacgtt gtagatgagc tcgccgtcct
     3541 ggaggctggt gtcctgggtg gcggtcagca cgcccccgtc ttcgaacacc atggttctct
     3601 cccatgtgaa gccctcaggg aaggactgct taaaataatc ggggaggccc ttggggtact
     3661 tgatgaaggc cttgctcccg tacataaaca tggtggccag gatgtcgaat gcgaacggca
     3721 gggggcctcc ctccaccacc ttgatcctgt tggtctgggt gccctcgtag ggcttgccct
     3781 ccccttcgtg ggtgcacttg aactggtggc cgttcacgct gccttccagg tacagcttgc
     3841 ttctcatgtt ctccttgatc agctcctcgc ccttgctcac catggtggcg gctagctcat
     3901 gcagatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
     3961 aggcagcctg cacctgagga gtgaattcct cgaggaattc tttgccaaaa tgatgagaca
     4021 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
     4081 tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc
     4141 ccccccgaag gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac
     4201 ggcggcgcgg cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc
     4261 gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac
     4321 agatttggga caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc
     4381 tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc ccctcccccg
     4441 agccctcccc ggcccgaggc ggccccgccc cgcccggcac ccccacctgc cgccaccccc
     4501 cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc
     4561 cgtgctcagc aactcgggga ggggggtgca gggggggttg cagcccgacc gacgcgccca
     4621 caccccctgc tcaccccccc acgcacacac cccgcacgca gcctttgttc ccctcgcagc
     4681 cccccccgca ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg
     4741 cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg
     4801 cggcgctccc cacgcacaca cacacgcacg caccccccga gccgctcccc ccgcacaaag
     4861 ggccctcccg gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt
     4921 aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga
     4981 gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg
     5041 ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc
     5101 gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg
     5161 ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc
     5221 gccccccccc cccccccgcc cccatcgctg cacaaaataa ttaaaaaata aataaataca
     5281 aaattggggg tggggagggg gggggagatg gggagagtga agcagaacgt ggggctcacc
     5341 tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     5401 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     5461 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     5521 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     5581 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     5641 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     5701 taataactag tcaataatca atgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     5761 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5821 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5881 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5941 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6001 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6061 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6121 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6181 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6241 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6301 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6361 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6421 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6481 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6541 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6601 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6661 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6721 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6781 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6841 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
