LOCUS       pBEAST_ahpCp-sfGFP      3947 bp ds-DNA     circular     10-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(893..921)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1193..1212
                     /label="pBEST for 3022->3041"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1247..1306
                     /label="pBEST 5' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1307..1346
                     /label="Spacer0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1347..1386
                     /label="Spacer3 (BglII)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      1387..1481
                     /label="B0014"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    1482..1521
                     /label="Spacer4 (BamhI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1522..1827
                     /label="ahpCp"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1801..1801
                     /label="+1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1804..1827)
                     /label="GG_218_toeholdins_RV"
                     /note="sequence: ccGGtctctTAAGCATCTATACTTCCTCCGTGTTTTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1828..1852
                     /label="RBS B034"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1853..1876
                     /label="pBeastGFP wo prom FW"
                     /note="sequence: CCGGTCTCtATGcgtaaaggcgaagagctgttc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1853..2572
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#d20ff7
                     /ApEinfo_fwdcolor=#d20ff7
     primer          1856..1873
                     /label="GG_218_toeholdins_FW"
                     /note="sequence: ccGGTCTCtTACTcgtaaaggcgaagagctg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2573..2591
                     /label="1586"
                     /note="sequence: GCGGTCTCAGCTTactttatctgagaatagtc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2573..2612
                     /label="Spacer5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2613..2618
                     /label="SphI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2619..2658
                     /label="Spacer5'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2659..2787
                     /label="B0015"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    2788..2827
                     /label="Spacer6 (EagI)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2828..2867
                     /label="SpacerN"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2868..2927
                     /label="pBEST 3' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(2969..2988)
                     /label="pBEST rev 870->851"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      3164..3783
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(join(3938..3947,1..851))
                     /label="Ampicillin/ORF frame 1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
       61 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      121 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      181 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      241 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      301 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
      361 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      421 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      481 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
      541 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      601 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      661 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      721 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      781 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
      841 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
      901 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
      961 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT
     1021 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAAGAAT TCTGGCGAAT CCTCTGACCA
     1081 GCCAGAAAAC GACCTTTCTG TGGTGAAACC GGATGCTGCA ATTCAGAGCG GCAGCAAGTG
     1141 GGGGACAGCA GAAGACCTGA CCGCCGCAGA GTGGATGTTT GACATGGTGA AGACTATCGC
     1201 ACCATCAGCC AGAAAACCGA ATTTTGCTGG GTGGGCTAAC GATATCcgcc tgatgcgtga
     1261 acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctggctcg gataccctta
     1321 ctctgttgaa aacgaataga taggttaagg aacggttatt tctgcgtaga tctatcttac
     1381 acagcatcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     1441 atataaaaag ccagattatt aatccggctt ttttattatt taggcaactg aaacgattcg
     1501 gatcctgtat tactattctt aGCTTAGATC AGGTGATTGC CCTTTGTTTA TGAGGGTGTT
     1561 GTAATCCATG TCGTTGTTGC ATTTGTAAGG GCAACACCTC AGCCTGCAGG CAGGCACTGA
     1621 AGATACCAAA GGGTAGTTCA GATTACACGG TCACCTGGAA AGGGGGCCAT TTTACTTTTT
     1681 ATCGCCGCTG GCGGTGCAAA GTTCACAAAG TTGTCTTACG AAGGTTGTAA GGTAAAACTT
     1741 ATCGATTTGA TAATGGAAAC GCATTAGCCG AATCGGCAAA AATTGGTTAC CTTACATCTC
     1801 ATCGAAAACA CGGAGGAAGT ATAGATGTCT AGAGAAAGAG GAGAAATACT AGatgcgtaa
     1861 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
     1921 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
     1981 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
     2041 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
     2101 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
     2161 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
     2221 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
     2281 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
     2341 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
     2401 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
     2461 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     2521 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatgat gaactttatc
     2581 tgagaatagt caatcttcgg aaatcccagg tggcatgcta aaagtctcgt aaagcgttct
     2641 atcaataacc cgttggtgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     2701 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2761 cttcgggtgg gcctttctgc gtttataccg tctcagaatc ggccgtgaac aataaaatag
     2821 tttcggtatt attgaccact tccgagtaga atcgtgcttc agtaagagtc gaccgatgcc
     2881 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcaTGA CTATCGTCGC
     2941 CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT
     3001 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG
     3061 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
     3121 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
     3181 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
     3241 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
     3301 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
     3361 TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     3421 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     3481 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     3541 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     3601 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     3661 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     3721 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     3781 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     3841 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT
     3901 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGC
//