LOCUS       pBEAST_YjjZ-sfGFP       3719 bp ds-DNA     circular     27-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          1..28
                     /label="YjjZ prom FW"
                     /note="sequence: CCGGTCTCtGAATcatagcgataggtttacccgatagcaag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     source          1..103
                     /label="source"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    7..23
                     /label="OxyR BS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    41..59
                     /label="Repressor Fur"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    70..88
                     /label="Repressor Fur"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          104..127
                     /label="pBeastGFP wo prom FW"
                     /note="sequence: CCGGTCTCtATGcgtaaaggcgaagagctgttc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             104..823
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#d20ff7
                     /ApEinfo_fwdcolor=#d20ff7
     primer          107..124
                     /label="GG_218_toeholdins_FW"
                     /note="sequence: ccGGTCTCtTACTcgtaaaggcgaagagctg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          824..842
                     /label="1586"
                     /note="sequence: GCGGTCTCAGCTTactttatctgagaatagtc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    824..863
                     /label="Spacer5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    864..869
                     /label="SphI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    870..909
                     /label="Spacer5'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      910..1038
                     /label="B0015"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    1039..1078
                     /label="Spacer6 (EagI)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1079..1118
                     /label="SpacerN"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1119..1178
                     /label="pBEST 3' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(1220..1239)
                     /label="pBEST rev 870->851"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      1415..2034
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2189..3049)
                     /label="Ampicillin/ORF frame 1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(3091..3119)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3391..3410
                     /label="pBEST for 3022->3041"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3445..3504
                     /label="pBEST 5' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3505..3544
                     /label="Spacer0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3545..3584
                     /label="Spacer3 (BglII)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      3585..3679
                     /label="B0014"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    3680..3719
                     /label="Spacer4 (BamhI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 catagcgata ggtttacccg atagcaaggg atttatctgg cttgcaaatg ataaaaatta
       61 tcatatgata ttggttatca ttatcaatga aagagatgaa atcatgcgta aaggcgaaga
      121 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      181 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      241 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      301 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      361 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      421 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      481 aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa
      541 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      601 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      661 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      721 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      781 agcgggcatc acgcatggta tggatgaact gtacaaatga tgaactttat ctgagaatag
      841 tcaatcttcg gaaatcccag gtggcatgct aaaagtctcg taaagcgttc tatcaataac
      901 ccgttggtgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      961 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1021 ggcctttctg cgtttatacc gtctcagaat cggccgtgaa caataaaata gtttcggtat
     1081 tattgaccac ttccgagtag aatcgtgctt cagtaagagt cgaccgatgc ccttgagagc
     1141 cttcaaccca gtcagctcct tccggtgggc gcggggcaTG ACTATCGTCG CCGCACTTAT
     1201 GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT
     1261 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
     1321 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
     1381 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
     1441 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
     1501 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
     1561 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
     1621 CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
     1681 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
     1741 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
     1801 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
     1861 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
     1921 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
     1981 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
     2041 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
     2101 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA
     2161 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT
     2221 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA
     2281 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT
     2341 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
     2401 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA
     2461 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT
     2521 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA
     2581 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA
     2641 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA
     2701 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT
     2761 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG
     2821 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC
     2881 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT
     2941 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA
     3001 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT
     3061 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT
     3121 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG
     3181 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
     3241 CCTTTCGTCT TCAAGAATTC TGGCGAATCC TCTGACCAGC CAGAAAACGA CCTTTCTGTG
     3301 GTGAAACCGG ATGCTGCAAT TCAGAGCGGC AGCAAGTGGG GGACAGCAGA AGACCTGACC
     3361 GCCGCAGAGT GGATGTTTGA CATGGTGAAG ACTATCGCAC CATCAGCCAG AAAACCGAAT
     3421 TTTGCTGGGT GGGCTAACGA TATCcgcctg atgcgtgaac gtgacggacg taaccaccgc
     3481 gacatgtgtg tgctgttccg ctggctcgga tacccttact ctgttgaaaa cgaatagata
     3541 ggttaaggaa cggttatttc tgcgtagatc tatcttacac agcatcacac tggctcacct
     3601 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa
     3661 tccggctttt ttattattta ggcaactgaa acgattcgga tcctgtatta ctattctta
//