LOCUS       T7-lox_in_pBeast        3924 bp ds-DNA     circular     04-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..18
                     /label="T7 promoter (pT7-7, Tabor2001)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     1..20
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    24..29
                     /label="NheI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          24..57
                     /label="1383"
                     /note="sequence: GCTAGCAATAATTTTGTTTAACTTTAAGAAGGAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(24..62)
                     /label="1657"
                     /note="sequence: GGTCTCATATATCTCCTTCTTAAAGTTAAACAAAATTATTGCTAGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(24..62)
                     /label="1385"
                     /note="sequence: TATATCTCCTTCTTAAAGTTAAACAAAATTATTGCTAGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    29..62
                     /label="rv"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          29..91
                     /label="Gibs_T7-lox_ins_fw"
                     /note="sequence: caataattttgtttaactttaagaaggagatataATGAATAACAATGACATTGAATATAATGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(29..91)
                     /label="Gibs_T7-lox_vec_RV"
                     /note="sequence: GCATTATATTCAATGTCATTGTTATTCATtatatctccttcttaaagttaaacaaaattattg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             30..62
                     /label="UTR1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(39..61)
                     /label="1587"
                     /note="sequence: GCGGTCTCGCATCatatctccttcttaaagttaaac"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(40..62)
                     /label="Gibson_pBEAST2-pT7-sfGFP_REV"
                     /note="sequence: ACATCAAAATGTGTTGACATtatatctccttcttaaagttaaa"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    63..78
                     /label="Fw"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    63..91
                     /label="fw"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    63..1187
                     /label="LOX"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             63..1187
                     /label="Translation 63-1187"
     misc_feature    72..100
                     /label="Fw lox"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    855..857
                     /label="GCA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    864..866
                     /label="GCA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1153..1180)
                     /label="Rv Lox"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1161..1187
                     /label="ins"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1161..1207
                     /label="Gibs_T7-lox_vec_FW"
                     /note="sequence: GATAACCCATACGGTTATGAATACTAGagcgttctatcaataacccg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1161..1207)
                     /label="Gibs_T7-lox_ins_RV"
                     /note="sequence: cgggttattgatagaacgctCTAGTATTCATAACCGTATGGGTTATC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1188..1207
                     /label="vec"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1188..1213
                     /label="Spacer5'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1188..1283
                     /label="IDT gBlock"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1214..1243
                     /label="T500 terminator"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1244..1283
                     /label="Spacer6 (EagI)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1284..1323
                     /label="SpacerN"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1324..1383
                     /label="pBEST 3' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(1425..1444)
                     /label="pBEST rev 870->851"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1617..2299)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      1620..2239
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2394..3254)
                     /label="Ampicillin/ORF frame 1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2397..3056)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(3296..3324)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3596..3615
                     /label="pBEST for 3022->3041"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3650..3709
                     /label="pBEST 5' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3710..3736
                     /label="33"
                     /note="sequence: CTCGGATACCCTTACTCTGTTGAAAAC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3710..3737)
                     /label="71"
                     /note="sequence: cgttttcaacagagtaagggtatccgag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3710..3749
                     /label="Spacer0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3750..3789
                     /label="Spacer3 (BglII)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      3790..3884
                     /label="B0014"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    3862..62
                     /label="IDT gBlock PVT1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3885..3924
                     /label="Spacer4 (BamhI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     3885..3907
                     /label="#37"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3893..3924)
                     /label="#38"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(3898..3924)
                     /label="GG_pbeast_noprom_RV"
                     /note="sequence: ccGGTCTCttaagaatagtaatacaggatccgaatc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 taatacgact cactataggg agagctagca ataattttgt ttaactttaa gaaggagata
       61 taATGAATAA CAATGACATT GAATATAATG CACCTAGTGA AATCAAGTAC ATTGATGTTG
      121 TCAATACTTA CGACTTAGAA GAAGAAGCAA GTAAAGTGGT ACCACATGGT GGTTTTAACT
      181 ATATTGCCGG TGCATCTGGT GATGAGTGGA CTAAACGCGC TAATGACCGT GCTTGGAAAC
      241 ATAAATTACT ATACCCACGT CTAGCGCAAG ATGTTGAAGC GCCCGATACA AGTACTGAAA
      301 TTTTAGGTCA TAAAATTAAA GCCCCATTCA TCATGGCACC AATTGCTGCA CATGGTTTAG
      361 CCCACACTAC TAAAGAAGCT GGTACTGCAC GTGCAGTTTC AGAATTTGGT ACAATTATGT
      421 CCATCTCAGC TTATTCTGGT GCAACATTTG AAGAAATTTC TGAAGGCTTA AATGGCGGAC
      481 CCCGTTGGTT CCAAATCTAT ATGGCTAAAG ATGACCAACA AAACCGTGAT ATCTTAGACG
      541 AAGCTAAATC TGATGGTGCA ACTGCTATCA TCCTTACAGC TGACTCAACT GTTTCTGGAA
      601 ACCGTGACCG TGATGTGAAG AATAAATTCG TTTACCCATT TGGTATGCCA ATTGTTCAAC
      661 GTTACTTACG TGGTACAGCA GAAGGTATGT CATTAAACAA TATCTACGGT GCTTCAAAAC
      721 AAAAAATCTC ACCAAGAGAT ATTGAGGAAA TCGCCGCTCA TTCTGGATTA CCAGTATTCG
      781 TTAAAGGTAT TCAACACCCA GAAGATGCAG ATATGGCAAT CAAAGCTGGT GCATCAGGTA
      841 TCTGGGTATC TAACCACGGT GCTCGTCAAC TATATGAAGC TCCAGGTTCA TTTGACACCC
      901 TTCCAGCTAT TGCTGAACGT GTAAACAAAC GTGTACCAAT CGTCTTTGAT TCAGGTGTAC
      961 GTCGTGGTGA ACACGTTGCC AAAGCGCTAG CTTCAGGGGC AGACGTTGTT GCTTTAGGAC
     1021 GCCCAGTCTT ATTTGGTTTA GCTTTAGGTG GCTGGCAAGG TGCTTACTCA GTACTTGACT
     1081 ACTTCCAAAA AGACTTAACA CGCGTAATGC AATTAACAGG TTCACAAAAT GTGGAAGACT
     1141 TGAAGGGTCT AGATTTATTC GATAACCCAT ACGGTTATGA ATACTAGagc gttctatcaa
     1201 taacccgttg gtgCAAAGCC CGCCGAAAGG CGGGCTTTTC TGTccgtctc agaatcggcc
     1261 gtgaacaata aaatagtttc ggtattattg accacttccg agtagaatcg tgcttcagta
     1321 agagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     1381 gcaTGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG
     1441 TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     1501 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     1561 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     1621 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     1681 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     1741 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     1801 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG
     1861 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     1921 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     1981 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     2041 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     2101 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     2161 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     2221 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2281 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2341 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2401 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2461 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2521 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2581 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2641 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2701 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2761 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2821 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2881 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2941 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     3001 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3061 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3121 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3181 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3241 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3301 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3361 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC
     3421 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AATTCTGGCG AATCCTCTGA
     3481 CCAGCCAGAA AACGACCTTT CTGTGGTGAA ACCGGATGCT GCAATTCAGA GCGGCAGCAA
     3541 GTGGGGGACA GCAGAAGACC TGACCGCCGC AGAGTGGATG TTTGACATGG TGAAGACTAT
     3601 CGCACCATCA GCCAGAAAAC CGAATTTTGC TGGGTGGGCT AACGATATCc gcctgatgcg
     3661 tgaacgtgac ggacgtaacc accgcgacat gtgtgtgctg ttccgctggc tcggataccc
     3721 ttactctgtt gaaaacgaat agataggtta aggaacggtt atttctgcgt agatctatct
     3781 tacacagcat cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag
     3841 agaatataaa aagccagatt attaatccgg cttttttatt atttaggcaa ctgaaacgat
     3901 tcggatcctg tattactatt ctta
//