LOCUS       ahpCp-LacZ              6302 bp ds-DNA     circular     04-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..306
                     /label="ahpCp"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    280..280
                     /label="+1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    307..331
                     /label="RBS B034"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(311..331)
                     /label="pBEAST_ahpCp-LacZ REV (1)"
                     /note="sequence: gaatccgtaatcatggtCATCTAGTATTTCTCCTCTTTCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          332..351
                     /label="pBEAST_ahpCp-LacZ FWD (2)"
                     /note="sequence: AGAAAGAGGAGAAATACTAGATGaccatgattacggattc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    332..3406
                     /label="LacZ"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(352..368)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(352..369)
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(361..383)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1112..1129
                     /label="LacZ seq for 798"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1750..1768
                     /label="Twist2-for primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1818..1836)
                     /label="Twist1 rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2584..2602
                     /label="Twist2B-for primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2633..2651)
                     /label="Twist2A-rev primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(3387..3406)
                     /label="pBEAST_ahpCp-LacZ REV (2)"
                     /note="sequence: tgactattctcagataaagtttatttttgacaccagacca"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3407..3428
                     /label="pBEAST_ahpCp-LacZ FWD (1)"
                     /note="sequence: tggtctggtgtcaaaaataaactttatctgagaatagtcaat"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3407..3446
                     /label="Spacer5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3447..3452
                     /label="SphI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3453..3492
                     /label="Spacer5'"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      3493..3621
                     /label="B0015"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    3622..3661
                     /label="Spacer6 (EagI)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3662..3701
                     /label="SpacerN"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3702..3761
                     /label="pBEST 3' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(3803..3822)
                     /label="pBEST rev 870->851"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      3998..4617
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(4772..5632)
                     /label="Ampicillin/ORF frame 1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(5674..5702)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5974..5993
                     /label="pBEST for 3022->3041"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6028..6087
                     /label="pBEST 5' overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6088..6127
                     /label="Spacer0"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6128..6167
                     /label="Spacer3 (BglII)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      6168..6262
                     /label="B0014"
                     /ApEinfo_revcolor=#f5010a
                     /ApEinfo_fwdcolor=#f5010a
     misc_feature    6263..6302
                     /label="Spacer4 (BamhI)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 GCTTAGATCA GGTGATTGCC CTTTGTTTAT GAGGGTGTTG TAATCCATGT CGTTGTTGCA
       61 TTTGTAAGGG CAACACCTCA GCCTGCAGGC AGGCACTGAA GATACCAAAG GGTAGTTCAG
      121 ATTACACGGT CACCTGGAAA GGGGGCCATT TTACTTTTTA TCGCCGCTGG CGGTGCAAAG
      181 TTCACAAAGT TGTCTTACGA AGGTTGTAAG GTAAAACTTA TCGATTTGAT AATGGAAACG
      241 CATTAGCCGA ATCGGCAAAA ATTGGTTACC TTACATCTCA TCGAAAACAC GGAGGAAGTA
      301 TAGATGTCTA GAGAAAGAGG AGAAATACTA GATGaccatg attacggatt cactggccgt
      361 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      421 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      481 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
      541 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
      601 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
      661 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
      721 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
      781 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc
      841 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct
      901 gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt
      961 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac
     1021 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga
     1081 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag
     1141 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt
     1201 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta
     1261 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga
     1321 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt
     1381 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga
     1441 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg
     1501 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt
     1561 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga
     1621 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa
     1681 tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga
     1741 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg
     1801 cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga
     1861 ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga
     1921 gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt
     1981 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga
     2041 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg
     2101 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc
     2161 cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt
     2221 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa
     2281 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc
     2341 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga
     2401 gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc
     2461 agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac
     2521 gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat
     2581 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg
     2641 gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct
     2701 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg
     2761 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac
     2821 acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt
     2881 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga
     2941 tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc
     3001 gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg
     3061 ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta
     3121 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca
     3181 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga
     3241 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt
     3301 ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct
     3361 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataaactt tatctgagaa
     3421 tagtcaatct tcggaaatcc caggtggcat gctaaaagtc tcgtaaagcg ttctatcaat
     3481 aacccgttgg tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     3541 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     3601 gtgggccttt ctgcgtttat accgtctcag aatcggccgt gaacaataaa atagtttcgg
     3661 tattattgac cacttccgag tagaatcgtg cttcagtaag agtcgaccga tgcccttgag
     3721 agccttcaac ccagtcagct ccttccggtg ggcgcggggc aTGACTATCG TCGCCGCACT
     3781 TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT
     3841 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     3901 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     3961 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     4021 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     4081 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     4141 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     4201 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     4261 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     4321 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     4381 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     4441 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     4501 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     4561 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     4621 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
     4681 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     4741 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     4801 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     4861 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
     4921 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     4981 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     5041 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     5101 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     5161 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     5221 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     5281 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     5341 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     5401 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     5461 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     5521 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     5581 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     5641 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     5701 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     5761 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
     5821 GGCCCTTTCG TCTTCAAGAA TTCTGGCGAA TCCTCTGACC AGCCAGAAAA CGACCTTTCT
     5881 GTGGTGAAAC CGGATGCTGC AATTCAGAGC GGCAGCAAGT GGGGGACAGC AGAAGACCTG
     5941 ACCGCCGCAG AGTGGATGTT TGACATGGTG AAGACTATCG CACCATCAGC CAGAAAACCG
     6001 AATTTTGCTG GGTGGGCTAA CGATATCcgc ctgatgcgtg aacgtgacgg acgtaaccac
     6061 cgcgacatgt gtgtgctgtt ccgctggctc ggataccctt actctgttga aaacgaatag
     6121 ataggttaag gaacggttat ttctgcgtag atctatctta cacagcatca cactggctca
     6181 ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat
     6241 taatccggct tttttattat ttaggcaact gaaacgattc ggatcctgta ttactattct
     6301 ta
//