LOCUS       pRT498_MBP_3xOtU        7193 bp DNA     circular SYN 01-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   user
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2022 from SnapGene Viewer 6.1.0
            https://www.snapgene.com
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..7193
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=source:synthetic DNA construct"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/locus_tag=source:synthetic DNA construct"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          1..4838
                     /mol_type="other DNA"
                     /label=source:recombinant plasmid
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=source:recombinant plasmid"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/locus_tag=source:recombinant plasmid"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=source:recombinant plasmid"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/locus_tag=source:recombinant plasmid"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     source          6948..7193
                     /mol_type="other DNA"
                     /label=source:recombinant plasmid(1)
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=source:recombinant plasmid"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/locus_tag=source:recombinant plasmid(1)"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=source:recombinant plasmid(1)"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/locus_tag=source:recombinant plasmid(1)"
                     /note="color: #ffffff"
                     /organism="recombinant plasmid"
     rep_origin      12..467
                     /direction=RIGHT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=f1 ori"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=f1 ori"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="color: #ffff00"
     CDS             complement(560..1375)
                     /codon_start=1
                     /locus_tag="KanR"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=KanR"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=KanR"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=ori"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=ori"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="color: #ffff00"
     misc_feature    2271..2413
                     /locus_tag="bom"
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=bom"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=bom"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="color: #a6acb3"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /locus_tag="rop"
                     /product="Rop protein, which maintains plasmids at low
                     copy number"
                     /label=rop
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=rop"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=rop"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3278..4360)
                     /codon_start=1
                     /gene="lacI"
                     /locus_tag="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=lacI"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=lacI"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4361..4438)
                     /gene="lacI"
                     /locus_tag="lacI promoter"
                     /label=lacI promoter
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=lacI promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=lacI promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4751..4769
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=T7 promoter"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    4770..4794
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=lac operator"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=lac operator"
                     /note="/ApEinfo_revcolor=pink"
                     /note="color: #31849b"
     RBS             4809..4831
                     /locus_tag="efficient ribosome binding site 
                     frombacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /label=efficient ribosome binding site from bacterioph
                     /label=efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=efficient ribosome binding site from 
                     bacteriophage  T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="/ApEinfo_revcolor=cyan"
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=efficient ribosome binding site from
                     bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
                     /note="/ApEinfo_revcolor=cyan"
                     /note="color: #a6acb3"
     CDS             4851..4868
                     /codon_start=1
                     /locus_tag="6xHis"
                     /label=6xHis
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=6xHis"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=6xHis"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="HHHHHH"
     CDS             4878..5978
                     /codon_start=1
                     /locus_tag="MBP"
                     /label=MBP
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=MBP"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=MBP"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGIDAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             6018..6038
                     /codon_start=1
                     /locus_tag="TEV cleavage site"
                     /label=TEV cleavage site
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=TEV cleavage site"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="color: #a6acb3"
                     /translation="ENLYFQG"
     CDS             6051..6059
                     /codon_start=1
                     /locus_tag="Cysteine handle"
                     /label=Cysteine handle
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=Cysteine handle"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="CGC"
     CDS             6069..6947
                     /codon_start=1
                     /locus_tag="3xOtUBD"
                     /label=3xOtUBD
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=3xOtUBD"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #993366"
                     /translation="VNFLSENRMITSAAAALADTLLKNNNRITEGVLVDRIFDNKILSV
                     QEKQQLLNNLLDNHIKENKSLTKESLTRMLASTHFVQQQANVLLNEKFNKGSGVNFLSE
                     NRMITSAAAALADTLLKNNNRITEGVLVDRIFDNKILSVQEKQQLLNNLLDNHIKENKS
                     LTKESLTRMLASTHFVQQQANVLLNEKFNKGSGVNFLSENRMITSAAAALADTLLKNNN
                     RITEGVLVDRIFDNKILSVQEKQQLLNNLLDNHIKENKSLTKESLTRMLASTHFVQQQA
                     NVLLNEKFNK*"
     CDS             7021..7044
                     /codon_start=1
                     /locus_tag="8xHis"
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=8xHis"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=8xHis"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="color: #cc99b2"
                     /translation="HHHHHHHH"
     terminator      7121..7168
                     /locus_tag="T7 terminator"
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}
                     width 5 offset 0"
                     /note="/ApEinfo_label=T7 terminator"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="color: #ffffff"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc
     3181 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     3241 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3361 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     3421 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     3481 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     3541 cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     3601 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     3661 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     3721 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     3781 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     3841 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     3901 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     3961 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     4021 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     4081 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     4141 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     4201 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     4261 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     4321 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     4381 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     4441 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     4501 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     4561 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     4621 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     4681 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat
     4741 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     4801 gaaataattt tgtttaactt taagaaggag atatatccat gaacaccatt catcaccatc
     4861 accatcacaa cactagtatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg
     4921 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta
     4981 aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg
     5041 gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg
     5101 gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct
     5161 gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat
     5221 cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg
     5281 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac
     5341 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg
     5401 gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct
     5461 tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag
     5521 aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca
     5581 acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac
     5641 catccaaacc gttcgttggc gtgctgagcg caggtattga cgccgccagt ccgaacaaag
     5701 agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta
     5761 ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag
     5821 atccacgtat tgccgccaca atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc
     5881 cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc
     5941 gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg actagtggat
     6001 ctggtggtgg tggcggcgaa aacctgtact tccagggtgg ttctcatatg tgtggatgtg
     6061 gatcaggagt taatttcctg agcgaaaaca gaatgattac ctctgccgct gcagccttgg
     6121 ctgatacttt gctgaagaac aacaacagaa tcacagaggg tgtgctggtt gatagaatct
     6181 tcgacaataa gattttgtcc gtgcaggaaa aacagcaatt gctgaacaat ttgctggaca
     6241 accatattaa ggaaaacaaa tctctgacta aagagtccct gacaagaatg ttggcttcta
     6301 cccactttgt tcagcaacag gcaaacgtgt tgctgaatga gaagttcaac aaaggatcag
     6361 gagtaaattt tctttctgaa aatagaatga taactagtgc tgctgcagct cttgcagata
     6421 cattattgaa gaataataac agaattaccg aaggtgtctt agtagatagg atttttgata
     6481 ataaaatttt gtctgtacaa gaaaagcaac aactacttaa taatttatta gataatcata
     6541 ttaaagaaaa taaatcttta actaaagaaa gtttaacaag aatgttggct agtacgcatt
     6601 ttgtgcagca gcaagctaat gttttgttaa atgaaaaatt taataaaggc tctggtgtaa
     6661 attttttgtc tgaaaatcgc atgattactt ctgccgcagc agctttggca gataccttgc
     6721 tgaagaacaa caatcgcatt acagaaggcg tcttagtgga ccgtatcttt gacaataaaa
     6781 tcttatcggt gcaagagaag caacagcttc tgaacaatct gttggacaac catattaaag
     6841 aaaacaaaag cctgacgaaa gagagcctga cgcgtatgtt ggctagtaca catttcgtgc
     6901 agcagcaggc aaacgtgttg cttaacgaga agttcaataa atagtaagga tccgaattct
     6961 gtacaggcct tggcgcgcct gcaggcgagc tccgtcgaca agcttgcggc cgcactcgag
     7021 caccaccacc accaccacca ccactaattg attaatacct aggctgctaa acaaagcccg
     7081 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     7141 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
//
