LOCUS       Exported                5310 bp ds-DNA     circular SYN 28-JUL-2022
DEFINITION  pJUL3120.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-PE2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5310)
  TITLE     Liu Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          715..2502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="/Color=#99ff99"
                     /note="/modified_by=jsfg"
                     /note="color: #a6acb3"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff"
     promoter        626..644
                     /label=T7 Promoter
                     /note="/created_by=Joung Lab"
                     /note="color: #ffffff"
     regulatory      649..660
                     /label=KOZAK
                     /note="/created_by=jsfg"
                     /note="color: #ffffff"
     misc_feature    658..714
                     /label=bpNLS
                     /label=nonstandard type:  NLS
                     /note="/created_by=jsfg"
                     /note="color: #a6acb3"
     variation       715..2502
                     /label=HERV-Kcon RT codon optimized
                     /label=nonstandard type:  mutation
                     /note="/Color=#ff17e1"
                     /note="/created_by=User"
                     /note="/modified_by=jsfg"
                     /note="color: #a6acb3"
     misc_feature    2503..2514
                     /label=linker
                     /label=nonstandard type:  linker
                     /note="/created_by=jsfg"
                     /note="/modified_by=jsfg"
                     /note="color: #a6acb3"
     misc_feature    2515..2565
                     /label=bpNLS
                     /label=nonstandard type:  NLS
                     /note="/created_by=jsfg"
                     /note="color: #a6acb3"
     regulatory      2566..2568
                     /label=STOP
                     /note="/Color=#000000"
                     /note="/created_by=jsfg"
                     /note="color: #ffffff"
     CDS             2574..2591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #993366"
                     /translation="HHHHHH"
     polyA_signal    2620..2844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    2939..2955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(2963..2993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    3008..3029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /note="color: #31849b"
     rep_origin      complement(3317..3905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/Color=#99ffff"
                     /note="/modified_by=jsfg"
                     /note="color: #ffff00"
     CDS             complement(4076..4936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="/Color=#ffdd55"
                     /note="/modified_by=jsfg"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4699..4718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        complement(4937..5041)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcaaaagc
      721 agaaaacgga gaaatagagt gtccttcctg ggcgctgcca cagtggaacc acctaagccc
      781 atccctctga catggaaaac agagaagcct gtgtgggtca accagtggcc tctgcctaag
      841 cagaagctgg aggctctcca cctgctggcc aacgagcagc ttgagaaggg ccacatcgag
      901 cccagcttta gcccttggaa cagccctgtg ttcgtgatcc agaagaagag cggcaagtgg
      961 cggatgctga cagatctgag agctgtgaac gccgtgatcc aacccatggg ccccctgcag
     1021 ccaggcctgc cttcccctgc tatgatccct aaagattggc ctctgatcat catcgacctg
     1081 aaagactgct tcttcacaat cccactcgcc gagcaggatt gcgagaagtt cgccttcacc
     1141 atccccgcca tcaacaacaa ggagcctgcc accagattcc agtggaaggt gctgcctcag
     1201 ggcatgctga attctccaac aatctgccag accttcgtgg gcagagctct gcagcctgtt
     1261 agagaaaaat tcagcgactg ctacatcatt cactacatcg atgacatcct gtgcgccgct
     1321 gaaaccaagg ataagttgat cgactgttac accttcctgc aagccgaggt ggccaatgcc
     1381 ggactggcta tcgcctctga taagatccag accagcacac ctttccacta cctgggcatg
     1441 cagatcgaga accggaagat caagccacag aaaatcgaga tcagaaagga caccctgaag
     1501 accctgaacg acttccagaa actcctgggg gatatcaact ggatcagacc taccctggga
     1561 atccctacgt acgccatgag caacctgttc agcatcctga ggggcgacag cgacctgaac
     1621 agcaagagaa tgctgacccc tgaggccaca aaagagatca agctggtgga agagaagatc
     1681 cagtctgctc aaatcaacag aatcgatccc ctggcccctc ttcagttgct gattttcgcc
     1741 actgcccata gccccaccgg cattatcatc cagaacaccg acctggtgga atggtctttt
     1801 ctgccccaca gcaccgtgaa gacatttaca ctgtacctgg accagatcgc caccctgatc
     1861 ggccaaacaa gactgcggat catcaagctg tgtggcaacg accccgacaa gatcgtggtg
     1921 cctctgacca aggaacaggt gcggcaggct tttattaact ccggcgcctg gcagatcgga
     1981 ctggccaact tcgttggcat catcgacaat cactatccta agaccaagat cttccaattt
     2041 ctgaagctga ccacctggat tctgcctaag attacaagac gggaacccct ggagaacgcc
     2101 ctgaccgtgt tcaccgacgg atcttccaac ggcaaagccg cctacaccgg ccctaaggaa
     2161 agagtgatta agacaccata ccagagcgcc cagagagccg aactggtcgc cgtgatcacc
     2221 gtgctgcagg acttcgacca gcctatcaat atcatcagcg acagtgccta tgtggtgcag
     2281 gccacccggg acgtggaaac cgccctgatc aagtacagca tggacgatca gctcaaccag
     2341 ctgtttaacc tgctgcagca gaccgtgcgg aagagaaact tccccttcta catcacccac
     2401 atccgcgccc acaccaacct gcccggccct ctgacaaagg ccaatgagca ggctgatctg
     2461 ctggtgtcta gcgccctgat taaggcccag gagctgcacg cctctggcgg ctcaaaaaga
     2521 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggtcatcatc
     2581 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     2641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2701 tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt cattctattc
     2761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2821 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     2881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     3001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac gaaaactcac
     3961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     5101 ccctagggtc tactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     5161 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     5221 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     5281 ttcgcgatgt acgggccaga tatacgcgtt
//
