LOCUS       Exported                7104 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Godoy Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..22
                     /label=GG1B
                     /note="GG1B"
     protein_bind    123..144
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        159..189
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=monomeric derivative of DsRed (Campbell et al.,
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1058..1085
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1083..1102)
                     /label=028
                     /note="Vector.REV,3/6/19,72.6,Nebuilder"
     misc_feature    1092..1113
                     /label=GG1B
                     /note="GG1B"
     CDS             complement(1120..1461)
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     regulatory      1551..1577
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     regulatory      1688..1713
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             1746..2015
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      2034..2054
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(2103..2378)
                     /codon_start=1
                     /product="putative RCR protein"
                     /label=putative RCR protein
                     /note="contains a domain belonging to RepB proteins; may
                     be involved in rolling circle replication (RCR)"
                     /protein_id="AUJ88097.1"
                     /translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLDS
                     QRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
     regulatory      2431..2457
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      2630..3966
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     CDS             complement(3730..3999)
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     CDS             complement(3989..4282)
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     regulatory      4316..4343
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             complement(4327..4887)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      5011..5040
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     primer_bind     complement(5021..5046)
                     /label=ori_pstI_f
     primer_bind     5021..5040
                     /label=028
                     /note="Vector.REV,3/6/19,72.6,Nebuilder"
     primer_bind     5041..5052
                     /label=027
                     /note="Vector.FOR,3/6/19,74.9,Nebuilder"
     primer_bind     complement(5184..5201)
                     /label=reverse_colo_pstI
     rep_origin      complement(5223..5811)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5982..6842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6843..6947)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     primer_bind     complement(6931..6947)
                     /label=024
                     /note="ab_ori_r_gent_eco,02/13/2019,64.6,Amplification of 
                     genes for MoClo"
     terminator      7037..7080
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     primer_bind     7037..7066
                     /label=021
                     /note="pBP_pTU_f,01/30/2019,59.2,Colony PCR moclo"
     misc_feature    7083..7102
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcatgc cctatagaga cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat aggtctcatg ttccggagag acgctgcagt taacaagttg cctgacgctg
     1141 tgtcttatcc agttcatgcg gcaatgctat ctctacttcc tttgctaatc ttgcctcctg
     1201 tttgatgtca ccgttctttt ttagttcgga ttgctctacc cgattccata gggtttgacg
     1261 atctaacatg tcaggacttg ccccagttgg ggcaaaaatt tgggtgtatt caatgcctgt
     1321 ttttttggtg tagtcctgct cttttccgta cgtatcacag tacaattttt cgcctgctcg
     1381 gtatgctgca catgccacga ttgagcgacc atctgacctc gaaatgttct gcatttcaca
     1441 atggtaaatt gccattttca tcacctgttt attaacagcc gccccaagtt ttgggcggtt
     1501 ggggtgtcgg ggtttccctg accgtacttg caaaatttgg cgtagcaaaa attttgcgta
     1561 agtgcgccct tcgggaactc tgcgaggcta aaagcaaaag caagagcaat aaggctttga
     1621 ctttgttttt tgattttgct cgctgcgctc ggatcttgat gtaagtcgag tttttgaagt
     1681 agaacacttt tcacattgat gatggttcat acttcggaaa ataggttgag acacaggcat
     1741 taaaaatggt caaaaaaaca gctatggaat tgggtcaaat gctcgatgaa gaaaaggaaa
     1801 aattggaacg tcaggaaaag gcaagaaagg atctagctga cgctgtggtg aaaggtcgag
     1861 agcaaaaaca ggcaaggtca gacgatgcaa aacgcaagat cctgatcggg gcttacttac
     1921 tgaagaaaca tgataatgac atcaagaaac ttgtggaaca aaaccccgat tttattgggt
     1981 acattagaga aaatgataag cacttatttt cataggcatt agtagttata aaaaaccctc
     2041 ataaatcgag ggtttctttt ttatctgcat tactttttga atcaatgtac ttttttagct
     2101 tgctattttt tcactgcata cagccgctta tactctttct caatcaatgc ggtcaaaatg
     2161 gctttttggc tctcccctgc tgcgcccacc atgtccgcaa gcatatccga cacaccctta
     2221 tctataaaca cctccaaacg ttgactatca aggcttttgc gtttttcacg atatgctttt
     2281 tgtcgttcgg cattggtttt tggcggctct tttagcatgt cctgtgtttt ctgatcgttc
     2341 gggtctttca tgtctttaac tcccatatag tatatcatag ttacaggata acattgtttt
     2401 tagttacagg ataacttttt ataaaattta tttgtaaaaa tttatgctat cctgcaacta
     2461 tttttaaatt acaggataac tatttaacgt taccctgtaa ctatagttat cctgtaacgc
     2521 taaattaatt accctgtaac atatattttg ttatcctgta acaactaaat attgaaataa
     2581 tactaccaaa gttaccctgt aacgaaaata actaggttaa aaaatgatcg gattttaaca
     2641 ttttgcgttg ttccaaaagt tatcaacagc ctagaacgtc ataggaagcg attacagaca
     2701 ctttatagct atcagcatgg gaacataagg gcaggatgaa atatgggtct taaaacgcaa
     2761 atggtgaggt tttagaggta ttttttgaag atgattaagg cggtttgttt ttaaaatttt
     2821 tggcggctct caggctgctt acattttaac cagttcagtg aaaagttctt tttcagcaaa
     2881 tttctgttta gcaccatagc taaaacttgc gtggaacata ttaagaattg accgaaatga
     2941 cacaatctca attatatttt ttttgaaaag ttttctttat caaatatttt aaatcattga
     3001 tttatatata agtatacatt cattttaata attaatcttt atttaacaat gatttatcta
     3061 tattcaattg tttaattatt cttactaata ttatctctat atcaatattt tttatttaaa
     3121 aacatatgtt tagtagtgct tttgattaaa gtaccagagg gagggagcag agctgaatgg
     3181 gaaatactca ccctagagcg attcttaaaa atcaccctaa agtattccca ttcgatgtac
     3241 cgtcggtcgg tcgctttcgc atcagggatg acatcactgt atcaagctgc cactgttatg
     3301 attacgattg atagcaccgc ctgaacacgc tcataaccgc caattaaatg actactcatt
     3361 gcgtccgcta ctctgttcag ttcccctagt aatagcgttt ttccgatgtg tgctagcgtc
     3421 actgtacctc atcacccaca catggacaga ttgagttaca gacattggct aaatttttgg
     3481 ggtctatgct tgacaaagcg agttaaaacc ttagaattta agacaggtac attaagcccc
     3541 tgtggtgaaa tcattagcgg tcgtcaaacc ttaatgcttt cgattgccga tatgtcagta
     3601 tcgaagatct gtaccccata aattacggta aagccccaag caattgcaag gggcttttat
     3661 cttttttaac aaaaaaaatt tataaatcag gattttataa cactaaataa tccaaagtac
     3721 atgagtaagt catgaccact cctgcgatgt gtgtagcact ctgagtatcc gtattatatc
     3781 agtgatgtca tatacaacca cataatgcgg atgtatcact aactccctag tatctttctg
     3841 tctgcctgtg cgccccatca gtcgattatc aacaagtaaa tctgtctttt cttcaattaa
     3901 atcatcaatt tcaatagctg ctatagggtt gcgttcttca agataatcat agatatttct
     3961 acgatcttgg cgagctgttt cagtccactc aatcttcatt cttcgctgcc agtttcgcca
     4021 atgttgcagc acgtctagcg gcaaactctg cctttacgtc ctcatgtgga atcaacttgc
     4081 ctgcattggc ttcatttaag ccgatctgta cctgctgctt gaaccatttg tcatagtctg
     4141 cggcttcctg ttgcttctta acaaagtcac gcatgaagtc acggattagc tgcgcccccg
     4201 accgatcaac agtcttagca aggcttgaaa attcttgctt taggtcatgg tctacccgaa
     4261 aggtaaaagt cgcttctgtc atgtttttca ctccaatgtg ttacatttga atgctattgt
     4321 aacacattaa aaagttaggt cattgtcttt tttcggctta ggctgttctt ggtcataacg
     4381 tggtttaaat gatggctgtc gctcattctc aagtaaaatt ttccgtttat taacagcctg
     4441 ttgcatctgc tcatagtaag gcatcaactg cggtaaaata tcagcaagtg cctgtctgtc
     4501 tttatccagg atacggctca attgcccgat ttttttcaag tcacgctcat aggctattct
     4561 gtcgctgatc tgctcgtatt tttgctttga acaccttatg tactcttgca tatcgttact
     4621 tgcccatgcc tgcgcctgaa gacgtgtaaa catggaaatg tctgaagcaa catggtcgct
     4681 gatatggtca tactcagcat ctttgacata gccgtatttt gataaatatc tttcatagtc
     4741 ctgccgtttc tttttgtcat cttcctgttt ttgtttagtt tctctttcta tctgctgttg
     4801 ttttcgttgc tgttcctgtt gctcctcttt tatctctctg tgataaatgg aaaggctaga
     4861 ttgatcatta tcccaacgtg tgagcatgtc ctcaaagccg taataatcag cactaaacca
     4921 acctgttttc actttcactg gtggcggcag tggctctccg atctccctta gcagtgctgt
     4981 agctgctcgt ccctctttga tcactttatc aagctgcaag cggtcgatat tgtcttgcaa
     5041 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5641 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     5701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5881 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6001 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6061 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6121 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6181 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6241 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6301 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6361 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6421 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6481 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6541 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6601 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6661 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6721 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6781 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6961 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7021 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     7081 tggaattcgc ggccgcttct agaa
//
