LOCUS       Exported                8092 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     154..179
                     /label=pEXR
     promoter        159..189
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1058..1085
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1120..1461)
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     regulatory      1551..1577
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     regulatory      1688..1713
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             1746..2015
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      2034..2054
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(2103..2378)
                     /codon_start=1
                     /product="putative RCR protein"
                     /label=putative RCR protein
                     /note="contains a domain belonging to RepB proteins; may
                     be involved in rolling circle replication (RCR)"
                     /protein_id="AUJ88097.1"
                     /translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLDS
                     QRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
     regulatory      2431..2457
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     regulatory      2630..3966
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     CDS             complement(3730..3999)
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     CDS             complement(3989..4282)
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     regulatory      4316..4343
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             complement(4327..4887)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      5011..5040
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     primer_bind     complement(5021..5046)
                     /label=ori_pstI_f
     terminator      5047..5104
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5080..5104)
                     /label=022
                     /note="pBP_r,01/30/2019,58,Colony PCR moclo"
     primer_bind     complement(5180..5199)
                     /label=VR
     primer_bind     complement(5260..5277)
                     /label=Primer 1
     rep_origin      complement(5299..5887)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      6070..6164
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     6139..6162
                     /label=pQErev
     primer_bind     6179..6202
                     /label=Kan_F_XhoI
     misc_feature    6184..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1061 selection-3"
     misc_feature    6184..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1058 selection"
     misc_feature    6184..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1055 rev selection selection"
     misc_feature    6184..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1053 selection"
     misc_feature    6184..7062
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1020 selection 2-4"
     misc_feature    6184..6187
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pEMKan selection selection"
     misc_feature    6188..7062
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from Synthetic NPT III"
     promoter        6201..6231
                     /note="amiA promoter fragment"
     promoter        6203..6208
                     /label=-35 predicted
     promoter        6223..6231
                     /label=-10 predicted
     RBS             6251..6255
     CDS             6268..7062
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-aminoglycoside phosphotransferase type III"
                     /label=Kanamycin Resistance (3',5'-aminoglycoside phos
                     /label=Kanamycin Resistance (3',5'-aminoglycoside 
                     phosphotransferase type III)
                     /note="kanamycin resistance protein; derived from the
                     aphIII gene of plasmid pJH1"
                     /db_xref="GI:33151036"
                     /protein_id="AAP97396.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7063..7065
                     /label=AatII
                     /label=nonstandard type:  Restriction_sit
                     /note="Name: AatII"
                     /note="Pattern: gacgtc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
     misc_feature    7063..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1031 selection-2"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1045 selection-2"
     terminator      7080..7166
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7084..7135
                     /label=rrnB T1
     primer_bind     complement(7146..7176)
                     /label=Kan_R_XhoI
     CDS             complement(7183..7842)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7954..7973
                     /label=VF2
     terminator      8026..8069
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     primer_bind     8026..8055
                     /label=021
                     /note="pBP_pTU_f,01/30/2019,59.2,Colony PCR moclo"
     misc_feature    8072..8092
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
ORIGIN
        1 ggtctcacta tatctagaga cgcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat acgtctcacc gggtacagag accctgcagt taacaagttg cctgacgctg
     1141 tgtcttatcc agttcatgcg gcaatgctat ctctacttcc tttgctaatc ttgcctcctg
     1201 tttgatgtca ccgttctttt ttagttcgga ttgctctacc cgattccata gggtttgacg
     1261 atctaacatg tcaggacttg ccccagttgg ggcaaaaatt tgggtgtatt caatgcctgt
     1321 ttttttggtg tagtcctgct cttttccgta cgtatcacag tacaattttt cgcctgctcg
     1381 gtatgctgca catgccacga ttgagcgacc atctgacctc gaaatgttct gcatttcaca
     1441 atggtaaatt gccattttca tcacctgttt attaacagcc gccccaagtt ttgggcggtt
     1501 ggggtgtcgg ggtttccctg accgtacttg caaaatttgg cgtagcaaaa attttgcgta
     1561 agtgcgccct tcgggaactc tgcgaggcta aaagcaaaag caagagcaat aaggctttga
     1621 ctttgttttt tgattttgct cgctgcgctc ggatcttgat gtaagtcgag tttttgaagt
     1681 agaacacttt tcacattgat gatggttcat acttcggaaa ataggttgag acacaggcat
     1741 taaaaatggt caaaaaaaca gctatggaat tgggtcaaat gctcgatgaa gaaaaggaaa
     1801 aattggaacg tcaggaaaag gcaagaaagg atctagctga cgctgtggtg aaaggtcgag
     1861 agcaaaaaca ggcaaggtca gacgatgcaa aacgcaagat cctgatcggg gcttacttac
     1921 tgaagaaaca tgataatgac atcaagaaac ttgtggaaca aaaccccgat tttattgggt
     1981 acattagaga aaatgataag cacttatttt cataggcatt agtagttata aaaaaccctc
     2041 ataaatcgag ggtttctttt ttatctgcat tactttttga atcaatgtac ttttttagct
     2101 tgctattttt tcactgcata cagccgctta tactctttct caatcaatgc ggtcaaaatg
     2161 gctttttggc tctcccctgc tgcgcccacc atgtccgcaa gcatatccga cacaccctta
     2221 tctataaaca cctccaaacg ttgactatca aggcttttgc gtttttcacg atatgctttt
     2281 tgtcgttcgg cattggtttt tggcggctct tttagcatgt cctgtgtttt ctgatcgttc
     2341 gggtctttca tgtctttaac tcccatatag tatatcatag ttacaggata acattgtttt
     2401 tagttacagg ataacttttt ataaaattta tttgtaaaaa tttatgctat cctgcaacta
     2461 tttttaaatt acaggataac tatttaacgt taccctgtaa ctatagttat cctgtaacgc
     2521 taaattaatt accctgtaac atatattttg ttatcctgta acaactaaat attgaaataa
     2581 tactaccaaa gttaccctgt aacgaaaata actaggttaa aaaatgatcg gattttaaca
     2641 ttttgcgttg ttccaaaagt tatcaacagc ctagaacgtc ataggaagcg attacagaca
     2701 ctttatagct atcagcatgg gaacataagg gcaggatgaa atatgggtct taaaacgcaa
     2761 atggtgaggt tttagaggta ttttttgaag atgattaagg cggtttgttt ttaaaatttt
     2821 tggcggctct caggctgctt acattttaac cagttcagtg aaaagttctt tttcagcaaa
     2881 tttctgttta gcaccatagc taaaacttgc gtggaacata ttaagaattg accgaaatga
     2941 cacaatctca attatatttt ttttgaaaag ttttctttat caaatatttt aaatcattga
     3001 tttatatata agtatacatt cattttaata attaatcttt atttaacaat gatttatcta
     3061 tattcaattg tttaattatt cttactaata ttatctctat atcaatattt tttatttaaa
     3121 aacatatgtt tagtagtgct tttgattaaa gtaccagagg gagggagcag agctgaatgg
     3181 gaaatactca ccctagagcg attcttaaaa atcaccctaa agtattccca ttcgatgtac
     3241 cgtcggtcgg tcgctttcgc atcagggatg acatcactgt atcaagctgc cactgttatg
     3301 attacgattg atagcaccgc ctgaacacgc tcataaccgc caattaaatg actactcatt
     3361 gcgtccgcta ctctgttcag ttcccctagt aatagcgttt ttccgatgtg tgctagcgtc
     3421 actgtacctc atcacccaca catggacaga ttgagttaca gacattggct aaatttttgg
     3481 ggtctatgct tgacaaagcg agttaaaacc ttagaattta agacaggtac attaagcccc
     3541 tgtggtgaaa tcattagcgg tcgtcaaacc ttaatgcttt cgattgccga tatgtcagta
     3601 tcgaagatct gtaccccata aattacggta aagccccaag caattgcaag gggcttttat
     3661 cttttttaac aaaaaaaatt tataaatcag gattttataa cactaaataa tccaaagtac
     3721 atgagtaagt catgaccact cctgcgatgt gtgtagcact ctgagtatcc gtattatatc
     3781 agtgatgtca tatacaacca cataatgcgg atgtatcact aactccctag tatctttctg
     3841 tctgcctgtg cgccccatca gtcgattatc aacaagtaaa tctgtctttt cttcaattaa
     3901 atcatcaatt tcaatagctg ctatagggtt gcgttcttca agataatcat agatatttct
     3961 acgatcttgg cgagctgttt cagtccactc aatcttcatt cttcgctgcc agtttcgcca
     4021 atgttgcagc acgtctagcg gcaaactctg cctttacgtc ctcatgtgga atcaacttgc
     4081 ctgcattggc ttcatttaag ccgatctgta cctgctgctt gaaccatttg tcatagtctg
     4141 cggcttcctg ttgcttctta acaaagtcac gcatgaagtc acggattagc tgcgcccccg
     4201 accgatcaac agtcttagca aggcttgaaa attcttgctt taggtcatgg tctacccgaa
     4261 aggtaaaagt cgcttctgtc atgtttttca ctccaatgtg ttacatttga atgctattgt
     4321 aacacattaa aaagttaggt cattgtcttt tttcggctta ggctgttctt ggtcataacg
     4381 tggtttaaat gatggctgtc gctcattctc aagtaaaatt ttccgtttat taacagcctg
     4441 ttgcatctgc tcatagtaag gcatcaactg cggtaaaata tcagcaagtg cctgtctgtc
     4501 tttatccagg atacggctca attgcccgat ttttttcaag tcacgctcat aggctattct
     4561 gtcgctgatc tgctcgtatt tttgctttga acaccttatg tactcttgca tatcgttact
     4621 tgcccatgcc tgcgcctgaa gacgtgtaaa catggaaatg tctgaagcaa catggtcgct
     4681 gatatggtca tactcagcat ctttgacata gccgtatttt gataaatatc tttcatagtc
     4741 ctgccgtttc tttttgtcat cttcctgttt ttgtttagtt tctctttcta tctgctgttg
     4801 ttttcgttgc tgttcctgtt gctcctcttt tatctctctg tgataaatgg aaaggctaga
     4861 ttgatcatta tcccaacgtg tgagcatgtc ctcaaagccg taataatcag cactaaacca
     4921 acctgttttc actttcactg gtggcggcag tggctctccg atctccctta gcagtgctgt
     4981 agctgctcgt ccctctttga tcactttatc aagctgcaag cggtcgatat tgtcttgcaa
     5041 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     5101 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5281 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     5341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     6061 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     6121 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     6181 tctcgagaag ctggggatcc gtttgatttt taatggataa tgtgatataa tctttaaata
     6241 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     6301 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     6361 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     6421 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     6481 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     6541 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     6601 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     6661 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     6721 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     6781 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     6841 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     6901 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     6961 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     7021 gagaaaataa aatattatat tttactggat gaattgtttt aggacgtcgc cggcggcatc
     7081 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     7141 tgaacgctct cctgagtagg acaaatcctc gagaatatca aattacgccc cgccctgcca
     7201 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     7261 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     7321 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     7381 ggtgaaactc acccagggat tggctgacac gaaaaacata ttctcaataa accctttagg
     7441 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     7501 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     7561 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     7621 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     7681 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     7741 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     7801 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     7861 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     7921 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     7981 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     8041 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta ga
//
