LOCUS       Exported                8092 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..22
                     /label=GG2D
                     /note="GG2D"
     protein_bind    123..144
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        159..189
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /label=monomeric derivative of DsRed
                     /note="mRFP1"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1058..1085
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    1092..1113
                     /label=GG2D
                     /note="GG2D"
     primer_bind     1114..1139
                     /label=ori_pstI_f
     regulatory      1120..1149
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             1273..1833
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      1817..1844
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             1878..2171
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             2161..2430
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      2194..3530
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     regulatory      3703..3729
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     CDS             3782..4057
                     /codon_start=1
                     /product="putative RCR protein"
                     /label=putative RCR protein
                     /note="contains a domain belonging to RepB proteins; may
                     be involved in rolling circle replication (RCR)"
                     /protein_id="AUJ88097.1"
                     /translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLDS
                     QRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
     regulatory      4106..4126
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(4145..4414)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      complement(4447..4472)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      4583..4609
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             4699..5040
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQATC"
     terminator      5047..5104
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(5299..5887)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      6070..6164
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     primer_bind     6179..6202
                     /label=Kan_F_XhoI
     misc_feature    6184..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1061 selection-3"
     misc_feature    6184..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1058 selection"
     misc_feature    6184..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1055 rev selection selection"
     misc_feature    6184..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1053 selection"
     misc_feature    6184..7062
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1020 selection 2-4"
     misc_feature    6184..6187
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pEMKan selection selection"
     misc_feature    6188..7062
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from Synthetic NPT III"
     promoter        6201..6231
                     /note="amiA promoter fragment"
     promoter        6203..6208
                     /label=-35 predicted
     promoter        6223..6231
                     /label=-10 predicted
     RBS             6251..6255
     CDS             6268..7062
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-aminoglycoside phosphotransferase type III"
                     /label=Kanamycin Resistance (3',5'-aminoglycoside phos
                     /label=Kanamycin Resistance (3',5'-aminoglycoside 
                     phosphotransferase type III)
                     /note="kanamycin resistance protein; derived from the
                     aphIII gene of plasmid pJH1"
                     /db_xref="GI:33151036"
                     /protein_id="AAP97396.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7063..7065
                     /label=AatII
                     /label=nonstandard type:  Restriction_sit
                     /note="Name: AatII"
                     /note="Pattern: gacgtc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
     misc_feature    7063..7065
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1031 selection-2"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pdl1013 selection rev"
     misc_feature    7066..7168
                     /label=Insert
                     /label=nonstandard type:  Insertion
                     /note="originates from pDL1045 selection-2"
     terminator      7080..7166
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7084..7135
                     /label=rrnB T1
     primer_bind     complement(7146..7176)
                     /label=Kan_R_XhoI
     CDS             complement(7183..7842)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      8026..8069
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    join(8072..8092,1)
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ""ATG"""
ORIGIN
        1 ggtctcatcg aatctagaga cgcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat acgtctcatt agtgttagag accctgcagt tgcaagacaa tatcgaccgc
     1141 ttgcagcttg ataaagtgat caaagaggga cgagcagcta cagcactgct aagggagatc
     1201 ggagagccac tgccgccacc agtgaaagtg aaaacaggtt ggtttagtgc tgattattac
     1261 ggctttgagg acatgctcac acgttgggat aatgatcaat ctagcctttc catttatcac
     1321 agagagataa aagaggagca acaggaacag caacgaaaac aacagcagat agaaagagaa
     1381 actaaacaaa aacaggaaga tgacaaaaag aaacggcagg actatgaaag atatttatca
     1441 aaatacggct atgtcaaaga tgctgagtat gaccatatca gcgaccatgt tgcttcagac
     1501 atttccatgt ttacacgtct tcaggcgcag gcatgggcaa gtaacgatat gcaagagtac
     1561 ataaggtgtt caaagcaaaa atacgagcag atcagcgaca gaatagccta tgagcgtgac
     1621 ttgaaaaaaa tcgggcaatt gagccgtatc ctggataaag acagacaggc acttgctgat
     1681 attttaccgc agttgatgcc ttactatgag cagatgcaac aggctgttaa taaacggaaa
     1741 attttacttg agaatgagcg acagccatca tttaaaccac gttatgacca agaacagcct
     1801 aagccgaaaa aagacaatga cctaactttt taatgtgtta caatagcatt caaatgtaac
     1861 acattggagt gaaaaacatg acagaagcga cttttacctt tcgggtagac catgacctaa
     1921 agcaagaatt ttcaagcctt gctaagactg ttgatcggtc gggggcgcag ctaatccgtg
     1981 acttcatgcg tgactttgtt aagaagcaac aggaagccgc agactatgac aaatggttca
     2041 agcagcaggt acagatcggc ttaaatgaag ccaatgcagg caagttgatt ccacatgagg
     2101 acgtaaaggc agagtttgcc gctagacgtg ctgcaacatt ggcgaaactg gcagcgaaga
     2161 atgaagattg agtggactga aacagctcgc caagatcgta gaaatatcta tgattatctt
     2221 gaagaacgca accctatagc agctattgaa attgatgatt taattgaaga aaagacagat
     2281 ttacttgttg ataatcgact gatggggcgc acaggcagac agaaagatac tagggagtta
     2341 gtgatacatc cgcattatgt ggttgtatat gacatcactg atataatacg gatactcaga
     2401 gtgctacaca catcgcagga gtggtcatga cttactcatg tactttggat tatttagtgt
     2461 tataaaatcc tgatttataa attttttttg ttaaaaaaga taaaagcccc ttgcaattgc
     2521 ttggggcttt accgtaattt atggggtaca gatcttcgat actgacatat cggcaatcga
     2581 aagcattaag gtttgacgac cgctaatgat ttcaccacag gggcttaatg tacctgtctt
     2641 aaattctaag gttttaactc gctttgtcaa gcatagaccc caaaaattta gccaatgtct
     2701 gtaactcaat ctgtccatgt gtgggtgatg aggtacagtg acgctagcac acatcggaaa
     2761 aacgctatta ctaggggaac tgaacagagt agcggacgca atgagtagtc atttaattgg
     2821 cggttatgag cgtgttcagg cggtgctatc aatcgtaatc ataacagtgg cagcttgata
     2881 cagtgatgtc atccctgatg cgaaagcgac cgaccgacgg tacatcgaat gggaatactt
     2941 tagggtgatt tttaagaatc gctctagggt gagtatttcc cattcagctc tgctccctcc
     3001 ctctggtact ttaatcaaaa gcactactaa acatatgttt ttaaataaaa aatattgata
     3061 tagagataat attagtaaga ataattaaac aattgaatat agataaatca ttgttaaata
     3121 aagattaatt attaaaatga atgtatactt atatataaat caatgattta aaatatttga
     3181 taaagaaaac ttttcaaaaa aaatataatt gagattgtgt catttcggtc aattcttaat
     3241 atgttccacg caagttttag ctatggtgct aaacagaaat ttgctgaaaa agaacttttc
     3301 actgaactgg ttaaaatgta agcagcctga gagccgccaa aaattttaaa aacaaaccgc
     3361 cttaatcatc ttcaaaaaat acctctaaaa cctcaccatt tgcgttttaa gacccatatt
     3421 tcatcctgcc cttatgttcc catgctgata gctataaagt gtctgtaatc gcttcctatg
     3481 acgttctagg ctgttgataa cttttggaac aacgcaaaat gttaaaatcc gatcattttt
     3541 taacctagtt attttcgtta cagggtaact ttggtagtat tatttcaata tttagttgtt
     3601 acaggataac aaaatatatg ttacagggta attaatttag cgttacagga taactatagt
     3661 tacagggtaa cgttaaatag ttatcctgta atttaaaaat agttgcagga tagcataaat
     3721 ttttacaaat aaattttata aaaagttatc ctgtaactaa aaacaatgtt atcctgtaac
     3781 tatgatatac tatatgggag ttaaagacat gaaagacccg aacgatcaga aaacacagga
     3841 catgctaaaa gagccgccaa aaaccaatgc cgaacgacaa aaagcatatc gtgaaaaacg
     3901 caaaagcctt gatagtcaac gtttggaggt gtttatagat aagggtgtgt cggatatgct
     3961 tgcggacatg gtgggcgcag caggggagag ccaaaaagcc attttgaccg cattgattga
     4021 gaaagagtat aagcggctgt atgcagtgaa aaaatagcaa gctaaaaaag tacattgatt
     4081 caaaaagtaa tgcagataaa aaagaaaccc tcgatttatg agggtttttt ataactacta
     4141 atgcctatga aaataagtgc ttatcatttt ctctaatgta cccaataaaa tcggggtttt
     4201 gttccacaag tttcttgatg tcattatcat gtttcttcag taagtaagcc ccgatcagga
     4261 tcttgcgttt tgcatcgtct gaccttgcct gtttttgctc tcgacctttc accacagcgt
     4321 cagctagatc ctttcttgcc ttttcctgac gttccaattt ttccttttct tcatcgagca
     4381 tttgacccaa ttccatagct gtttttttga ccatttttaa tgcctgtgtc tcaacctatt
     4441 ttccgaagta tgaaccatca tcaatgtgaa aagtgttcta cttcaaaaac tcgacttaca
     4501 tcaagatccg agcgcagcga gcaaaatcaa aaaacaaagt caaagcctta ttgctcttgc
     4561 ttttgctttt agcctcgcag agttcccgaa gggcgcactt acgcaaaatt tttgctacgc
     4621 caaattttgc aagtacggtc agggaaaccc cgacacccca accgcccaaa acttggggcg
     4681 gctgttaata aacaggtgat gaaaatggca atttaccatt gtgaaatgca gaacatttcg
     4741 aggtcagatg gtcgctcaat cgtggcatgt gcagcatacc gagcaggcga aaaattgtac
     4801 tgtgatacgt acggaaaaga gcaggactac accaaaaaaa caggcattga atacacccaa
     4861 atttttgccc caactggggc aagtcctgac atgttagatc gtcaaaccct atggaatcgg
     4921 gtagagcaat ccgaactaaa aaagaacggt gacatcaaac aggaggcaag attagcaaag
     4981 gaagtagaga tagcattgcc gcatgaactg gataagacac agcgtcaggc aacttgttaa
     5041 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     5101 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5281 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     5341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     6061 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     6121 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     6181 tctcgagaag ctggggatcc gtttgatttt taatggataa tgtgatataa tctttaaata
     6241 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
     6301 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     6361 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     6421 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     6481 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     6541 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     6601 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     6661 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     6721 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     6781 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     6841 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     6901 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     6961 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     7021 gagaaaataa aatattatat tttactggat gaattgtttt aggacgtcgc cggcggcatc
     7081 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     7141 tgaacgctct cctgagtagg acaaatcctc gagaatatca aattacgccc cgccctgcca
     7201 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     7261 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     7321 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     7381 ggtgaaactc acccagggat tggctgacac gaaaaacata ttctcaataa accctttagg
     7441 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     7501 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     7561 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     7621 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     7681 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     7741 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     7801 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     7861 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     7921 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     7981 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     8041 aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta ga
//
