LOCUS       Exported               11052 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..11
                     /label=GG1A
                     /note="GG1A"
     primer_bind     12..40
                     /label=PTet_f
     CDS             complement(16..639)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     547..566
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     657..680
                     /label=Primer 1
     promoter        658..713
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(702..735)
                     /label=PTet_R
     misc_feature    731..757
                     /label=PET-RBS
                     /note="PET-RBS"
                     /note="pET3a RBS"
     primer_bind     757..789
                     /label=pCas9_new_f
     CDS             762..4868
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(922..941)
                     /label=pBP_cas9_mut_r
     primer_bind     942..961
                     /label=pBP_cas9_mut_f
     primer_bind     complement(4849..4873)
                     /label=pCas9_new_r
     repeat_region   4870..4873
                     /label=GG scar
                     /note="GG scar"
     terminator      4881..4908
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4915..4929
                     /label=GG1A
                     /note="GG1A"
     primer_bind     complement(4919..4934)
                     /label=pTU_r_insert
     primer_bind     4930..4955
                     /label=ori_pstI_f
     regulatory      4936..4965
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             5089..5649
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      5633..5660
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             5694..5987
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             5977..6246
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      6010..7346
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     regulatory      7519..7545
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     CDS             7598..7873
                     /codon_start=1
                     /product="putative RCR protein"
                     /label=putative RCR protein
                     /note="contains a domain belonging to RepB proteins; may
                     be involved in rolling circle replication (RCR)"
                     /protein_id="AUJ88097.1"
                     /translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLDS
                     QRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
     regulatory      7922..7942
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(7961..8230)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      complement(8263..8288)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      8399..8425
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             8515..8988
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQALVTELCQSLVKAYGVAVDVAIHAPHVHGGRRKKPHAHIRHRRQATC"
     primer_bind     complement(8969..8994)
                     /label=ori_pstI_r
     primer_bind     complement(9024..9037)
                     /label=23
     rep_origin      complement(9171..9759)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9930..10790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10791..10895)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     terminator      10985..11028
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     primer_bind     10985..11014
                     /label=21
     primer_bind     10985..11014
                     /label=colo_f
     misc_feature    11031..11050
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tctatttaag acccactttc acatttaagt tgtttttcta atccgcatat
       61 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      121 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      181 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      241 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      301 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      361 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      421 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      481 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      541 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      601 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      661 gacactctat cgttgataga gttattttac cactccctat cagtgataga gaaaagaatt
      721 caaaagatct gtactttaac tttaagaagg agatatacat aatggataag aaatactcaa
      781 taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg
      841 ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc
      901 ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt cttaaacgga
      961 cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt
     1021 caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg
     1081 tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg
     1141 cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg
     1201 ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc
     1261 attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc
     1321 agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag
     1381 atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg
     1441 ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg
     1501 gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt
     1561 caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg
     1621 ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa
     1681 gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg
     1741 aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt
     1801 ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag
     1861 ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg
     1921 aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca
     1981 acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag
     2041 aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc
     2101 gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc
     2161 ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt
     2221 cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag
     2281 tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg
     2341 tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag
     2401 ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag
     2461 attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat
     2521 ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt
     2581 tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg
     2641 aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg
     2701 tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga
     2761 ttaatggtat tagggataag caatctggca aaacaatatt agattttttg aaatcagatg
     2821 gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag
     2881 acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt
     2941 tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat
     3001 tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa
     3061 atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag
     3121 gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc
     3181 aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag
     3241 aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc
     3301 ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat
     3361 cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc
     3421 taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag
     3481 gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa
     3541 tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg
     3601 ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc
     3661 gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg
     3721 cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg
     3781 agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc
     3841 aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca
     3901 aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg
     3961 gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat
     4021 tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca
     4081 aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg
     4141 atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg
     4201 ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca
     4261 caattatgga aagaagttcc tttgaaaaaa atccgattga ctttttagaa gctaaaggat
     4321 ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag
     4381 aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg
     4441 ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg
     4501 gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag
     4561 atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt
     4621 tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag
     4681 aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt
     4741 ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc
     4801 ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag
     4861 gtgactaact cgatcacact ggctcacctt cgggtgggcc tttctgcgtt tatatgtttg
     4921 ccagagacgc tgcagttgca agacaatatc gaccgcttgc agcttgataa agtgatcaaa
     4981 gagggacgag cagctacagc actgctaagg gagatcggag agccactgcc gccaccagtg
     5041 aaagtgaaaa caggttggtt tagtgctgat tattacggct ttgaggacat gctcacacgt
     5101 tgggataatg atcaatctag cctttccatt tatcacagag agataaaaga ggagcaacag
     5161 gaacagcaac gaaaacaaca gcagatagaa agagaaacta aacaaaaaca ggaagatgac
     5221 aaaaagaaac ggcaggacta tgaaagatat ttatcaaaat acggctatgt caaagatgct
     5281 gagtatgacc atatcagcga ccatgttgct tcagacattt ccatgtttac acgtcttcag
     5341 gcgcaggcat gggcaagtaa cgatatgcaa gagtacataa ggtgttcaaa gcaaaaatac
     5401 gagcagatca gcgacagaat agcctatgag cgtgacttga aaaaaatcgg gcaattgagc
     5461 cgtatcctgg ataaagacag acaggcactt gctgatattt taccgcagtt gatgccttac
     5521 tatgagcaga tgcaacaggc tgttaataaa cggaaaattt tacttgagaa tgagcgacag
     5581 ccatcattta aaccacgtta tgaccaagaa cagcctaagc cgaaaaaaga caatgaccta
     5641 actttttaat gtgttacaat agcattcaaa tgtaacacat tggagtgaaa aacatgacag
     5701 aagcgacttt tacctttcgg gtagaccatg acctaaagca agaattttca agccttgcta
     5761 agactgttga tcggtcgggg gcgcagctaa tccgtgactt catgcgtgac tttgttaaga
     5821 agcaacagga agccgcagac tatgacaaat ggttcaagca gcaggtacag atcggcttaa
     5881 atgaagccaa tgcaggcaag ttgattccac atgaggacgt aaaggcagag tttgccgcta
     5941 gacgtgctgc aacattggcg aaactggcag cgaagaatga agattgagtg gactgaaaca
     6001 gctcgccaag atcgtagaaa tatctatgat tatcttgaag aacgcaaccc tatagcagct
     6061 attgaaattg atgatttaat tgaagaaaag acagatttac ttgttgataa tcgactgatg
     6121 gggcgcacag gcagacagaa agatactagg gagttagtga tacatccgca ttatgtggtt
     6181 gtatatgaca tcactgatat aatacggata ctcagagtgc tacacacatc gcaggagtgg
     6241 tcatgactta ctcatgtact ttggattatt tagtgttata aaatcctgat ttataaattt
     6301 tttttgttaa aaaagataaa agccccttgc aattgcttgg ggctttaccg taatttatgg
     6361 ggtacagatc ttcgatactg acatatcggc aatcgaaagc attaaggttt gacgaccgct
     6421 aatgatttca ccacaggggc ttaatgtacc tgtcttaaat tctaaggttt taactcgctt
     6481 tgtcaagcat agaccccaaa aatttagcca atgtctgtaa ctcaatctgt ccatgtgtgg
     6541 gtgatgaggt acagtgacgc tagcacacat cggaaaaacg ctattactag gggaactgaa
     6601 cagagtagcg gacgcaatga gtagtcattt aattggcggt tatgagcgtg ttcaggcggt
     6661 gctatcaatc gtaatcataa cagtggcagc ttgatacagt gatgtcatcc ctgatgcgaa
     6721 agcgaccgac cgacggtaca tcgaatggga atactttagg gtgattttta agaatcgctc
     6781 tagggtgagt atttcccatt cagctctgct ccctccctct ggtactttaa tcaaaagcac
     6841 tactaaacat atgtttttaa ataaaaaata ttgatataga gataatatta gtaagaataa
     6901 ttaaacaatt gaatatagat aaatcattgt taaataaaga ttaattatta aaatgaatgt
     6961 atacttatat ataaatcaat gatttaaaat atttgataaa gaaaactttt caaaaaaaat
     7021 ataattgaga ttgtgtcatt tcggtcaatt cttaatatgt tccacgcaag ttttagctat
     7081 ggtgctaaac agaaatttgc tgaaaaagaa cttttcactg aactggttaa aatgtaagca
     7141 gcctgagagc cgccaaaaat tttaaaaaca aaccgcctta atcatcttca aaaaatacct
     7201 ctaaaacctc accatttgcg ttttaagacc catatttcat cctgccctta tgttcccatg
     7261 ctgatagcta taaagtgtct gtaatcgctt cctatgacgt tctaggctgt tgataacttt
     7321 tggaacaacg caaaatgtta aaatccgatc attttttaac ctagttattt tcgttacagg
     7381 gtaactttgg tagtattatt tcaatattta gttgttacag gataacaaaa tatatgttac
     7441 agggtaatta atttagcgtt acaggataac tatagttaca gggtaacgtt aaatagttat
     7501 cctgtaattt aaaaatagtt gcaggatagc ataaattttt acaaataaat tttataaaaa
     7561 gttatcctgt aactaaaaac aatgttatcc tgtaactatg atatactata tgggagttaa
     7621 agacatgaaa gacccgaacg atcagaaaac acaggacatg ctaaaagagc cgccaaaaac
     7681 caatgccgaa cgacaaaaag catatcgtga aaaacgcaaa agccttgata gtcaacgttt
     7741 ggaggtgttt atagataagg gtgtgtcgga tatgcttgcg gacatggtgg gcgcagcagg
     7801 ggagagccaa aaagccattt tgaccgcatt gattgagaaa gagtataagc ggctgtatgc
     7861 agtgaaaaaa tagcaagcta aaaaagtaca ttgattcaaa aagtaatgca gataaaaaag
     7921 aaaccctcga tttatgaggg ttttttataa ctactaatgc ctatgaaaat aagtgcttat
     7981 cattttctct aatgtaccca ataaaatcgg ggttttgttc cacaagtttc ttgatgtcat
     8041 tatcatgttt cttcagtaag taagccccga tcaggatctt gcgttttgca tcgtctgacc
     8101 ttgcctgttt ttgctctcga cctttcacca cagcgtcagc tagatccttt cttgcctttt
     8161 cctgacgttc caatttttcc ttttcttcat cgagcatttg acccaattcc atagctgttt
     8221 ttttgaccat ttttaatgcc tgtgtctcaa cctattttcc gaagtatgaa ccatcatcaa
     8281 tgtgaaaagt gttctacttc aaaaactcga cttacatcaa gatccgagcg cagcgagcaa
     8341 aatcaaaaaa caaagtcaaa gccttattgc tcttgctttt gcttttagcc tcgcagagtt
     8401 cccgaagggc gcacttacgc aaaatttttg ctacgccaaa ttttgcaagt acggtcaggg
     8461 aaaccccgac accccaaccg cccaaaactt ggggcggctg ttaataaaca ggtgatgaaa
     8521 atggcaattt accattgtga aatgcagaac atttcgaggt cagatggtcg ctcaatcgtg
     8581 gcatgtgcag cataccgagc aggcgaaaaa ttgtactgtg atacgtacgg aaaagagcag
     8641 gactacacca aaaaaacagg cattgaatac acccaaattt ttgccccaac tggggcaagt
     8701 cctgacatgt tagatcgtca aaccctatgg aatcgggtag agcaatccga actaaaaaag
     8761 aacggtgaca tcaaacagga ggcaagatta gcaaaggaag tagagatagc attgccgcat
     8821 gaactggata agacacagcg tcaggcactt gttaccgagt tgtgccagtc cttagttaaa
     8881 gcctatggag tggcggtgga cgtagcgatc catgcccctc atgtgcatgg gggaaggaga
     8941 aagaaacctc acgcccacat aagacacagg cgtcaggcaa cttgttaact gcaggcttcc
     9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9601 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agagccacgc
    10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10921 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    10981 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
    11041 ccgcttctag aa
//
