LOCUS       Exported               11127 bp ds-DNA     circular SYN 26-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Merlin Brychcy
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 29, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..11
                     /label=GG1A
                     /note="GG1A"
     primer_bind     12..40
                     /label=PTet_f
     CDS             complement(16..639)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     547..566
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     657..680
                     /label=Primer 1
     promoter        658..713
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(702..735)
                     /label=PTet_R
     misc_feature    731..757
                     /label=Tag_linker
                     /note="Tag_linker"
     primer_bind     758..833
                     /label=3xFlag_f
     primer_bind     complement(762..837)
                     /label=3x_FLAG_r
     CDS             765..830
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     834..864
                     /label=dCas9_f_for_frameshift
     CDS             837..4943
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     837..864
                     /label=pCas9_new_f
     primer_bind     complement(997..1016)
                     /label=pBP_cas9_mut_r
     primer_bind     1017..1036
                     /label=pBP_cas9_mut_f
     primer_bind     complement(4924..4948)
                     /label=pCas9_new_r
     repeat_region   4945..4948
                     /label=GG scar
                     /note="GG scar"
     terminator      4956..4983
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4990..5004
                     /label=GG1A
                     /note="GG1A"
     primer_bind     complement(4994..5009)
                     /label=pTU_r_insert
     primer_bind     5005..5030
                     /label=ori_pstI_f
     regulatory      5011..5040
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     CDS             5164..5724
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88093.1"
                     /translation="MLTRWDNDQSSLSIYHREIKEEQQEQQRKQQQIERETKQKQEDDK
                     KKRQDYERYLSKYGYVKDAEYDHISDHVASDISMFTRLQAQAWASNDMQEYIRCSKQKY
                     EQISDRIAYERDLKKIGQLSRILDKDRQALADILPQLMPYYEQMQQAVNKRKILLENER
                     QPSFKPRYDQEQPKPKKDNDLTF"
     regulatory      5708..5735
                     /regulatory_class="promoter"
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
     CDS             5769..6062
                     /codon_start=1
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88095.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             6052..6321
                     /codon_start=1
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="AUJ88096.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     misc_feature    6058..6315
                     /note="ParE toxin of type II toxin-antitoxin system,
                     parDE; Region: ParE_toxin; pfam05016"
                     /db_xref="CDD:309933"
     regulatory      6085..7421
                     /regulatory_class="replication_regulatory_region"
                     /note="minimal origin of replication for Acinetobacter
                     sp."
     regulatory      7594..7620
                     /regulatory_class="promoter"
                     /label=putative RCR protein gene predicted promoter
                     /note="putative RCR protein gene predicted promoter"
     CDS             7673..7948
                     /codon_start=1
                     /product="putative RCR protein"
                     /label=putative RCR protein
                     /note="contains a domain belonging to RepB proteins; may
                     be involved in rolling circle replication (RCR)"
                     /protein_id="AUJ88097.1"
                     /translation="MIYYMGVKDMKDPNDQKTQDMLKEPPKTNAERQKAYREKRKSLDS
                     QRLEVFIDKGVSDMLADMVGAAGESQKAILTALIEKEYKRLYAVKK"
     regulatory      7997..8017
                     /regulatory_class="terminator"
                     /note="bidirectional Rho-independent transcriptional
                     terminator"
     CDS             complement(8036..8305)
                     /codon_start=1
                     /product="putative MobA/MobL protein"
                     /label=putative MobA/MobL protein
                     /note="contains MobA/MobL family domain; may be involved
                     in plasmid mobilization"
                     /protein_id="AUJ88098.1"
                     /translation="MVKKTAMELGQMLDEEKEKLERQEKARKDLADAVVKGREQKQARS
                     DDAKRKILIGAYLLKKHDNDIKKLVEQNPDFIGYIRENDKHLFS"
     regulatory      complement(8338..8363)
                     /regulatory_class="promoter"
                     /label=putative MobA/MobL gene predicted promoter
                     /note="putative MobA/MobL gene predicted promoter"
     regulatory      8474..8500
                     /regulatory_class="promoter"
                     /label=putative nickase-like gene predicted promoter
                     /note="putative nickase-like gene predicted promoter"
     CDS             8590..9063
                     /codon_start=1
                     /product="putative nickase-like protein"
                     /label=putative nickase-like protein
                     /note="may be involved in rolling circle replication
                     (RCR)"
                     /protein_id="AUJ88099.1"
                     /translation="MKMAIYHCEMQNISRSDGRSIVACAAYRAGEKLYCDTYGKEQDYT
                     KKTGIEYTQIFAPTGASPDMLDRQTLWNRVEQSELKKNGDIKQEARLAKEVEIALPHEL
                     DKTQRQALVTELCQSLVKAYGVAVDVAIHAPHVHGGRRKKPHAHIRHRRQATC"
     primer_bind     complement(9044..9069)
                     /label=ori_pstI_r
     primer_bind     complement(9099..9112)
                     /label=23
     primer_bind     complement(9127..9146)
                     /label=VR
     rep_origin      complement(9246..9834)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10005..10865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10866..10970)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     primer_bind     10988..11007
                     /label=VF2
     terminator      11060..11103
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     primer_bind     11060..11089
                     /label=21
     primer_bind     11060..11089
                     /label=colo_f
     misc_feature    11106..11125
                     /label=BioBrick prefix
                     /note="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tctatttaag acccactttc acatttaagt tgtttttcta atccgcatat
       61 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      121 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      181 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      241 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      301 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      361 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      421 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      481 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      541 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      601 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      661 gacactctat cgttgataga gttattttac cactccctat cagtgataga gaaaagaatt
      721 caaaagatct gtactttaac tttaagaagg agatatataa aatggactac aaagaccatg
      781 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag ctccatatgg
      841 ataagaaata ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca
      901 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
      961 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     1021 ctcgtcttaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     1081 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     1141 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     1201 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     1261 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     1321 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     1381 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     1441 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     1501 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     1561 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     1621 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     1681 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     1741 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     1801 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     1861 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     1921 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     1981 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     2041 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     2101 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     2161 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     2221 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     2281 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     2341 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     2401 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     2461 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     2521 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     2581 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     2641 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     2701 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     2761 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     2821 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     2881 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     2941 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     3001 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     3061 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     3121 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     3181 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     3241 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     3301 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg
     3361 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     3421 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     3481 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     3541 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     3601 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     3661 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     3721 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     3781 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     3841 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     3901 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     3961 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     4021 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     4081 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     4141 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
     4201 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
     4261 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
     4321 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
     4381 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
     4441 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
     4501 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
     4561 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
     4621 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
     4681 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
     4741 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
     4801 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
     4861 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
     4921 tgagtcagct aggaggtgac taactcgatc acactggctc accttcgggt gggcctttct
     4981 gcgtttatat gtttgccaga gacgctgcag ttgcaagaca atatcgaccg cttgcagctt
     5041 gataaagtga tcaaagaggg acgagcagct acagcactgc taagggagat cggagagcca
     5101 ctgccgccac cagtgaaagt gaaaacaggt tggtttagtg ctgattatta cggctttgag
     5161 gacatgctca cacgttggga taatgatcaa tctagccttt ccatttatca cagagagata
     5221 aaagaggagc aacaggaaca gcaacgaaaa caacagcaga tagaaagaga aactaaacaa
     5281 aaacaggaag atgacaaaaa gaaacggcag gactatgaaa gatatttatc aaaatacggc
     5341 tatgtcaaag atgctgagta tgaccatatc agcgaccatg ttgcttcaga catttccatg
     5401 tttacacgtc ttcaggcgca ggcatgggca agtaacgata tgcaagagta cataaggtgt
     5461 tcaaagcaaa aatacgagca gatcagcgac agaatagcct atgagcgtga cttgaaaaaa
     5521 atcgggcaat tgagccgtat cctggataaa gacagacagg cacttgctga tattttaccg
     5581 cagttgatgc cttactatga gcagatgcaa caggctgtta ataaacggaa aattttactt
     5641 gagaatgagc gacagccatc atttaaacca cgttatgacc aagaacagcc taagccgaaa
     5701 aaagacaatg acctaacttt ttaatgtgtt acaatagcat tcaaatgtaa cacattggag
     5761 tgaaaaacat gacagaagcg acttttacct ttcgggtaga ccatgaccta aagcaagaat
     5821 tttcaagcct tgctaagact gttgatcggt cgggggcgca gctaatccgt gacttcatgc
     5881 gtgactttgt taagaagcaa caggaagccg cagactatga caaatggttc aagcagcagg
     5941 tacagatcgg cttaaatgaa gccaatgcag gcaagttgat tccacatgag gacgtaaagg
     6001 cagagtttgc cgctagacgt gctgcaacat tggcgaaact ggcagcgaag aatgaagatt
     6061 gagtggactg aaacagctcg ccaagatcgt agaaatatct atgattatct tgaagaacgc
     6121 aaccctatag cagctattga aattgatgat ttaattgaag aaaagacaga tttacttgtt
     6181 gataatcgac tgatggggcg cacaggcaga cagaaagata ctagggagtt agtgatacat
     6241 ccgcattatg tggttgtata tgacatcact gatataatac ggatactcag agtgctacac
     6301 acatcgcagg agtggtcatg acttactcat gtactttgga ttatttagtg ttataaaatc
     6361 ctgatttata aatttttttt gttaaaaaag ataaaagccc cttgcaattg cttggggctt
     6421 taccgtaatt tatggggtac agatcttcga tactgacata tcggcaatcg aaagcattaa
     6481 ggtttgacga ccgctaatga tttcaccaca ggggcttaat gtacctgtct taaattctaa
     6541 ggttttaact cgctttgtca agcatagacc ccaaaaattt agccaatgtc tgtaactcaa
     6601 tctgtccatg tgtgggtgat gaggtacagt gacgctagca cacatcggaa aaacgctatt
     6661 actaggggaa ctgaacagag tagcggacgc aatgagtagt catttaattg gcggttatga
     6721 gcgtgttcag gcggtgctat caatcgtaat cataacagtg gcagcttgat acagtgatgt
     6781 catccctgat gcgaaagcga ccgaccgacg gtacatcgaa tgggaatact ttagggtgat
     6841 ttttaagaat cgctctaggg tgagtatttc ccattcagct ctgctccctc cctctggtac
     6901 tttaatcaaa agcactacta aacatatgtt tttaaataaa aaatattgat atagagataa
     6961 tattagtaag aataattaaa caattgaata tagataaatc attgttaaat aaagattaat
     7021 tattaaaatg aatgtatact tatatataaa tcaatgattt aaaatatttg ataaagaaaa
     7081 cttttcaaaa aaaatataat tgagattgtg tcatttcggt caattcttaa tatgttccac
     7141 gcaagtttta gctatggtgc taaacagaaa tttgctgaaa aagaactttt cactgaactg
     7201 gttaaaatgt aagcagcctg agagccgcca aaaattttaa aaacaaaccg ccttaatcat
     7261 cttcaaaaaa tacctctaaa acctcaccat ttgcgtttta agacccatat ttcatcctgc
     7321 ccttatgttc ccatgctgat agctataaag tgtctgtaat cgcttcctat gacgttctag
     7381 gctgttgata acttttggaa caacgcaaaa tgttaaaatc cgatcatttt ttaacctagt
     7441 tattttcgtt acagggtaac tttggtagta ttatttcaat atttagttgt tacaggataa
     7501 caaaatatat gttacagggt aattaattta gcgttacagg ataactatag ttacagggta
     7561 acgttaaata gttatcctgt aatttaaaaa tagttgcagg atagcataaa tttttacaaa
     7621 taaattttat aaaaagttat cctgtaacta aaaacaatgt tatcctgtaa ctatgatata
     7681 ctatatggga gttaaagaca tgaaagaccc gaacgatcag aaaacacagg acatgctaaa
     7741 agagccgcca aaaaccaatg ccgaacgaca aaaagcatat cgtgaaaaac gcaaaagcct
     7801 tgatagtcaa cgtttggagg tgtttataga taagggtgtg tcggatatgc ttgcggacat
     7861 ggtgggcgca gcaggggaga gccaaaaagc cattttgacc gcattgattg agaaagagta
     7921 taagcggctg tatgcagtga aaaaatagca agctaaaaaa gtacattgat tcaaaaagta
     7981 atgcagataa aaaagaaacc ctcgatttat gagggttttt tataactact aatgcctatg
     8041 aaaataagtg cttatcattt tctctaatgt acccaataaa atcggggttt tgttccacaa
     8101 gtttcttgat gtcattatca tgtttcttca gtaagtaagc cccgatcagg atcttgcgtt
     8161 ttgcatcgtc tgaccttgcc tgtttttgct ctcgaccttt caccacagcg tcagctagat
     8221 cctttcttgc cttttcctga cgttccaatt tttccttttc ttcatcgagc atttgaccca
     8281 attccatagc tgtttttttg accattttta atgcctgtgt ctcaacctat tttccgaagt
     8341 atgaaccatc atcaatgtga aaagtgttct acttcaaaaa ctcgacttac atcaagatcc
     8401 gagcgcagcg agcaaaatca aaaaacaaag tcaaagcctt attgctcttg cttttgcttt
     8461 tagcctcgca gagttcccga agggcgcact tacgcaaaat ttttgctacg ccaaattttg
     8521 caagtacggt cagggaaacc ccgacacccc aaccgcccaa aacttggggc ggctgttaat
     8581 aaacaggtga tgaaaatggc aatttaccat tgtgaaatgc agaacatttc gaggtcagat
     8641 ggtcgctcaa tcgtggcatg tgcagcatac cgagcaggcg aaaaattgta ctgtgatacg
     8701 tacggaaaag agcaggacta caccaaaaaa acaggcattg aatacaccca aatttttgcc
     8761 ccaactgggg caagtcctga catgttagat cgtcaaaccc tatggaatcg ggtagagcaa
     8821 tccgaactaa aaaagaacgg tgacatcaaa caggaggcaa gattagcaaa ggaagtagag
     8881 atagcattgc cgcatgaact ggataagaca cagcgtcagg cacttgttac cgagttgtgc
     8941 cagtccttag ttaaagccta tggagtggcg gtggacgtag cgatccatgc ccctcatgtg
     9001 catgggggaa ggagaaagaa acctcacgcc cacataagac acaggcgtca ggcaacttgt
     9061 taactgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9121 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9181 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9661 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9721 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9961 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10021 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10081 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10141 ccgcgagagc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10201 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10261 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10321 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10381 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10441 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10501 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10561 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10621 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10681 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10741 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10801 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10861 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10921 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10981 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    11041 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
    11101 ttctggaatt cgcggccgct tctagaa
//
