LOCUS       Exported                6211 bp ds-DNA     circular SYN 16-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   JBZ
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Aug 16, 2022 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     primer_bind     140..170
                     /label=BZ308 InfusionF
                     /note="color: black; sequence: 
                     ctgcggccgcacgcgtGAGCTCCTTCCTGCT; added: 2019-03-06"
     misc_feature    156..2108
                     /label=MBPpromoter
                     /note="Goz... & Lazzarini 1991 MBP promoter, from 
                     PMID:1383235"
                     /note="color: #ff99cc"
     primer_bind     complement(474..490)
                     /label=BZ051
                     /note="color: black; sequence: GAGTCAGAGATGCAACC; added: 
                     2019-03-10"
     primer_bind     1203..1222
                     /label=BZ303
                     /note="color: black; sequence: GAGGGAATGGACTTGCTGCC; added:
                     2019-03-10"
     primer_bind     complement(1331..1346)
                     /label=BZ049
                     /note="color: black; sequence: GACGTGCTTACTCGGG; added: 
                     2019-03-10"
     primer_bind     1883..1901
                     /label=BZ304
                     /note="color: black; sequence: CAATGGGACCTCGGCTGAG; added: 
                     2019-03-10"
     primer_bind     1999..2016
                     /label=BZ050
                     /note="color: black; sequence: GCCTCTTTTCCCGAGATG; added: 
                     2019-03-10"
     misc_feature    2145..2151
                     /label=Kozak
                     /note="color: #ffff00"
     regulatory      2146..2155
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2152..2868
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2152 .. 2154 / #05fd14
                        2: 2155 .. 2157 / #05fd14 / 1a
                        3: 2158 .. 2868 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2197..2218)
                     /label=GFPReverse
                     /note="Sequetech"
                     /note="color: green; sequence: CGTCGCCGTCCAGCTCGACCAG; 
                     added: 2019-03-18"
     CDS             2884..2943
                     /codon_start=1
                     /label=Farnesyl tag
                     /note="color: #ff0000"
                     /translation="KLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(2925..2965)
                     /label=BZ309 InfusionR
                     /note="color: black; sequence: 
                     tgccacccgtagatctTTATCAGGAGAGCACACACTTGCAG; added: 
                     2019-03-06"
     polyA_signal    2958..3434
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(3018..3041)
                     /label=C-CMV-24 seq primer
                     /note="Sequetech"
                     /note="color: #a6acb3; direction: LEFT"
     repeat_region   3474..3614
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      3689..4144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4426..4530
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4531..5391
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4531 .. 4599 / #ccffcc / signal sequence
                        2: 4600 .. 5391 / #ccffcc
                     Cleavage site after base 4599"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5562..6150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgagct ccttcctgct taggccgtgt
      181 ctgctctgtc agtctttcct ctggctctag tctgtccatc tgcagggata acctcccaag
      241 ggccctcttg ttggctgttc accttccgtc tgtcttcata gcctcctgat gagagagcgc
      301 gcacacacag gctacctgac tgatgtgttg ctgcactgtg gggtccagtg acacagctgt
      361 ggaggtcagg aacccagaat taagctgtct gggagccaca agtttcaaga gtccatgcta
      421 tggctcctac atgcaggtgg tgccagcagc attccttggt gtcctgtggc tgtggttgca
      481 tctctgactc tcctccacct atcacattcc ttctccagta ccaggcctct gtgtctcagt
      541 tttcctctct cacaagatcc ctagcttcag gattgcaccc acctccacta gccatgagcc
      601 cgtcctcact ggatcacgtc tgcaaagact ctatttccag ttaaagtcac agacacgggc
      661 atagagaggg ggataggata tgaaaatatc agttggggga ggggcattca attcttagct
      721 cacattttct tcatccaatt tcccccacta gaatgtaagc tttgagagaa aagggaccag
      781 atcttattcc tcaccgtggc tttaacactt agagaaaatg catcccctct aatcaataag
      841 tcatcgacag tgggtagatg gaggaacggc agtgcgtagt aggatgcgtg caagcatagt
      901 ctcgtgcatg ggtgcataga tcgctgggca ggtggacaag gtgggggtgg ataaagaagt
      961 gggtagatga ttgatgttag gtaaatatca ctgggtggac agatgggtgg taggtggatg
     1021 gatggttaga atagtcagaa gagggatgga ttgataaggt gaacagatga taaatgggtg
     1081 atagactgga agggttgtca aaagaggata agggaagtgt gagctagccg tatttctaag
     1141 gtcagtaata gagttgggag aagaggttaa gttacatcca tttaaacctc acacgaagct
     1201 gagagggaat ggacttgctg ccgttggtga ggaaagcgtt gcatttcccg tgtgcttggt
     1261 tgtgaagtgc tcaggtccca catgaagcag tcaggttact gcggcttaca gaggagccag
     1321 atccaaatgc cccgagtaag cacgtccccg agccagaggc ctccagcgga atccgggaga
     1381 gggattgctc agtgccctgc ttccctggac tgtaagctgc agaaagatgt gggaagtcct
     1441 gttctccact gagaacacta aaagcacctt ttgtcaaacg accgcttcac atctggggct
     1501 tgtgcactgg tggcctttta aaccagagac aacccacaag atacctaacc tgcggggctc
     1561 tctggtacag tgagcaactc aggaaatgct ttggcttgat tgctgtgggc tctcaggcca
     1621 tcgccctctg gagtggttct tttaatgaga acctgaagat tggcccctga gccatgtata
     1681 ccaagcaagc tcaatccagg ttagctccct ctggttgggg caagctaacg tgctccttgg
     1741 gccccgcgcg taactgtgcg ttttatagga gacagctagt tcaagacccc aggaagaaag
     1801 cggctttgtc cccctctagg cctcgtacag gcccacattc atatctcatt gttgttgcag
     1861 gggaggcaga tgcgatccag aacaatggga cctcggctga ggacacggcg gtgacagact
     1921 ccaagcacac agcagaccca aagaataact ggcaaggcgc ccacccagct gacccaggga
     1981 accgccccca cttgatccgc ctcttttccc gagatgcccc gggaagggag gacaacacct
     2041 tcaaagacag gccctcagag tccgacgagc ttcagaccat ccaagaagac cccacagcag
     2101 cttccggaag ctcgaataat tctaggatcc ctagcgctac cggtcgccac catggtgagc
     2161 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2221 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2281 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2341 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2401 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2461 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2521 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2581 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2641 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2701 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2761 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2821 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     2881 tctaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc
     2941 tcctgataaa gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3001 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     3061 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     3121 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     3181 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     3241 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     3301 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     3361 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     3421 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     3481 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     3541 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     3601 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3661 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3901 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4021 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4141 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4201 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4261 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4321 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4381 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4441 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4501 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4561 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4621 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4681 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4741 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4801 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4861 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4921 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4981 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5041 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5401 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5701 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6181 ctggcctttt gctggccttt tgctcacatg t
//
