LOCUS       pEBO2(hyg)-D95N-        8967 bp    DNA     circular SYN 08-AUG-2012
DEFINITION  Ligation of rps9 cut with SacI and Xhoi into PZ467 cut with SacI
            and XhoI
ACCESSION   pEBO2(hyg)-D95N-
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    61..442
                     /gene="CMV enhancer"
     misc_feature    65..1793
                     /gene="CAGGS promoter"
     misc_signal     65..1793
                     /label=CAGGS promotor
     misc_feature    443..722
                     /gene="chicken ß-actin promoter"
     misc_feature    631..635
                     /gene="CAAT signal"
     CDS             1832..2416
                     /gene="rps9"
     misc_feature    2116
                     /gene="D95N"
     misc_feature    complement(2455..2976)
                     /gene="IRES"
     misc_signal     complement(2455..2976)
                     /label=IRES
     misc_signal     complement(2456..2461)
                     /label=Insertion
     CDS             3049..3768
                     /gene="eGFP"
     misc_signal     3117
                     /label=Mut.C-T
     misc_signal     3410
                     /label=Mut.A-G
     misc_signal     3743
                     /label=Mut.T-A
     misc_feature    3799..4424
                     /gene="hGH polyA"
     misc_feature    complement(4516..4564)
                     /gene="PolyA"
     CDS             complement(4588..5625)
                     /gene="HygR(euk)"
     misc_feature    complement(5650..6068)
                     /gene="SV40 prom"
     misc_feature    complement(6596..7036)
                     /gene="ori"
     CDS             complement(7334..8194)
                     /gene="AmpR"
ORIGIN      
        1 gggcgcgcca gcccgggctg gccggcctgc gatcgctgat atcgtcgcga cgaattcact
       61 cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      121 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      181 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      241 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      301 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      361 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      421 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      481 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      541 atgggggcgg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      601 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      961 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     1021 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1081 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1141 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1201 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1261 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1321 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1381 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1441 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1561 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1621 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1681 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1741 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc accgatcaga
     1801 agcttcgtta actgagctca ggatccatac catgccggtc gccagaagct gggtttgtcg
     1861 caaaacctat gtgaccccac ggagaccctt cgagaagtcg cgtctcgacc aggagctaaa
     1921 gttgattgga gagtatggac tccggaacaa acgtgaggtt tggagggtca agtttaccct
     1981 ggccaagatc cgtaaggcgg cccgggagct gttgacgcta gacgagaagg atccccggcg
     2041 tctgtttgaa ggcaatgctc tcctgcggcg gcttgttcgc attggggtgc tggacgaggg
     2101 caagatgaag ctgaactaca tcctgggcct gaagattgag gatttcttgg agaggcggct
     2161 gcagacccag gtctttaagc tgggcctggc caaatctatt caccatgccc gtgtgctcat
     2221 ccgccaacgt cacattaggg tccgcaagca ggtggtgaac atcccatcct tcattgttcg
     2281 cctggactct cagaagcaca tcgacttctc cctccgttct ccttatggcg gcggccgtcc
     2341 aggccgagtg aagaggaaga atgccaagaa aggccagggc ggggctggag ctggtgatga
     2401 tgaggaagag gattaactcg agggccggcg cgccgcggcc gctacgtaaa ttccgccccc
     2461 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     2521 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     2581 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     2641 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     2701 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     2761 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     2821 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ntcctcaagc gtattcaaca
     2881 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     2941 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     3001 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag
     3061 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3241 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggc gaaccgcatc
     3421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3481 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3541 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3661 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3721 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cgcctgtaca
     3781 gtggtcgacg attcgataag cttgatgggt ggcatccctg tgacccctcc ccagtgcctc
     3841 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     3901 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     3961 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     4021 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     4081 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     4141 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     4201 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     4261 ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag gaggacgtcc
     4321 agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt tgcttgcttg
     4381 gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattc ctgcagcccc
     4441 ctacctgcag gtacgcgtag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     4501 agcatctagt tcgaacacac aaaaaaccaa cacacagatg taatgaaaat aaagatattt
     4561 tattgcggcc atgaccggtt ctagagttta aactcgacct acctccttag cgcgcggtcg
     4621 tgtactgggc cggcggttgc cgctgtcggc cagcacctcg acgcagccgt cggtccatac
     4681 ggctgcgctc cggcgagcga tttgtgtgcg accgacggtg ccggccccgc tgcggacgat
     4741 ggcatcgcag cggccttgag cccaggcagc atcgtcgaag ttgccgtcca cgaggctctg
     4801 gtagagctga tccaggccga tgcgcagcat gtaggctcgc agacgagggc tgccggccag
     4861 ctcgggatgc cggcgctcga agtagcgagt ctgctgctcc atgcaagcca gccagggccg
     4921 ccagaagaag atgttggcca cctcgtactg actgtccccg aacatagctt cggaccagtc
     4981 gattacggcg gtgatgcggc cgttgtcggt caggacgttg ttgctgccga agtcggcatg
     5041 gacgaggtgg cgcacctcgg gacagtcttc ggcccacagc atcagttcgt ccagggcttg
     5101 agctacgctg gcggacacgg tgtcgtccat cacggtctgc cagtggtaga catggggatc
     5161 agcaatggcg caaatgaaat cccgccaggt tgtgtactgg ccgatgcctt ggggcccgaa
     5221 tgggccgaag ccgctggttt gactgaggtc ggcggcggca atagcatcca tagcttcggc
     5281 gacaggctgt aacacagcag gcagctctgt ttcggggagg tcttggagag taacgccttg
     5341 ggcgcgtcta ctgatgcagt atgtcaggct ctcgctgaac tcgccgatgt ccaacacttc
     5401 ggggatgggt agtgcagcgc tggcgaagtg gcggtacacg tagcggtctt tgtagaagcc
     5461 atcagcgcag ctattcaccc gcagtacata gccgcgtccg ccgacatcga agctgaaggc
     5521 tcggctctct tcgccctccg acaactgcat caggtcgctc acactgtcga acttctcgat
     5581 gagaaatttt tcaacgctgg tagcggtgag ttcgggcttc ttcatggtgg cgctagtgtc
     5641 agaagaatcg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5701 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5761 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5821 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5881 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5941 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     6001 ctttccgcct cagaaggtag ctaaccaagt tcctctttca gaggttattt caggccatgg
     6061 tgctgcgcag atcagcggaa gagcgcccaa tacgcgtcgt ttaaactcac gtggttaatt
     6121 aatccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     6181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7081 cttgatccgg caaacaaacc accgctggta gcgggtggtt ttttttgttt gcaagcagca
     7141 gattacgcgc agaaaaaaag gatctcaaga gatcctttga tcttttctac ggggtctgac
     7201 gctcagtgac gaaactcacg ttaaagggat tttggtcatg agatatcaaa aggatcttca
     7261 cctagatcct tttaaattta aaatgaagtt taaatcaatc taaagtaaaa tatgagtaaa
     7321 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7381 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7441 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7501 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7561 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7621 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7681 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7741 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7801 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7861 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7921 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7981 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8041 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8101 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8161 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     8341 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8401 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8461 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8521 aaccgtctat cagggcgatg gcccacgcgg ggactaccta tcagtttttt ggggtcgagg
     8581 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8641 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8701 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8761 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8821 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8881 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     8941 taatacgact cactataggg cgaattg
//
