LOCUS       Exported                9995 bp ds-DNA     circular SYN 05-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 5, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        402..781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        782..985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     936..956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    998..1017
                     /label=T7 promoter (modified for CleanCap from TriLink)
     CDS             1074..1127
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             1128..5228
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5229..5327
                     /codon_start=1
                     /label=Linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGSS"
     CDS             5328..7358
                     /codon_start=1
                     /label=MLV-RT
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     primer_bind     6338..6355
                     /label=ZG513
     primer_bind     complement(6347..6367)
                     /label=ZG512
     CDS             7401..7421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7430..7447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7456..7483)
                     /label=ZG511
     polyA_site      7460..7515
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    7671..7722
                     /label=PolyA (50-As)
     primer_bind     complement(7774..7790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7798..7814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7822..7852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7867..7888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8176..8764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8935..9795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9796..9900)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgacattgat tattgactag
      421 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      481 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      541 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      601 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      661 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      721 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      781 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      841 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      901 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      961 gtgggaggtc tatataagca gagctgagct cggtacctaa tacgactcac tataaggaaa
     1021 taagagagaa aagaagagta agaagaaata tagctagcag agccgccacc atgaaacgga
     1081 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
     1141 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1201 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     1261 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1321 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1381 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1441 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1501 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1561 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1621 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1681 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1741 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1801 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1861 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1921 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1981 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2041 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2101 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2161 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     2221 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     2281 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     2341 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2401 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2461 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2521 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2581 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2641 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2701 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2761 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2821 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2881 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2941 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3001 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3061 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3121 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3181 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     3241 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3301 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3361 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3421 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3481 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3541 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3601 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3661 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3721 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3781 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3841 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3901 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3961 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4021 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4081 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4141 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4201 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     4261 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4321 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4381 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4441 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4501 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4561 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4621 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4681 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4741 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4801 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4861 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4921 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4981 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5041 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5101 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5161 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     5221 gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg
     5281 agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc ctgaacatcg
     5341 aagatgagta cagactgcac gagacatcta aggaacctga tgtgtccctg ggttctacat
     5401 ggctgagcga cttccctcag gcctgggctg aaaccggcgg catgggcctg gctgtgcggc
     5461 aggctcctct gatcatccct ctgaaggcca cctccacccc tgtgtccatc aagcagtacc
     5521 ccatgagcca ggaggccaga ctgggcatca agccccacat ccagcggctg ctcgaccagg
     5581 gaatcctggt cccctgccag agcccctgga acaccccact tttacctgtg aaaaagcccg
     5641 gcactaacga ctaccggcct gtgcaggacc tgagagaagt gaacaagcgg gttgaggaca
     5701 tccaccctac agtgcctaac ccctacaacc tgctgtctgg cctgccaccg agccaccagt
     5761 ggtacaccgt gctggacctg aaagacgcct tcttctgtct gagactccat cctaccagcc
     5821 agccactgtt cgccttcgag tggcgggacc ctgagatggg catcagcggc cagctgacat
     5881 ggacccggct gccacagggc ttcaagaaca gccctacact gttcaacgag gccctgcaca
     5941 gagatctggc tgacttcaga atccagcatc ctgacctgat cctgctgcag tacgtggacg
     6001 acttgctgct tgccgctaca tctgaactgg attgccagca aggcaccaga gctctgctcc
     6061 agaccctggg caacctgggg tatagggcca gcgccaagaa ggcccagatc tgccagaagc
     6121 aagtgaagta cctgggatac ctgctgaagg aaggccagag atggctgacc gaggcgcgga
     6181 aggaaacagt gatgggccag cctaccccta agacccccag acagctgcga gaattcctgg
     6241 gcaaggctgg cttttgcaga ctgttcatcc ccggcttcgc tgagatggcc gctcctctgt
     6301 acccactgac caagcccggt accctgttca attggggccc cgatcagcag aaagcctacc
     6361 aggagattaa gcaggccctg ctgaccgccc ctgccctcgg gctgcctgat ctgacaaaac
     6421 ctttcgagct gtttgtcgac gagaagcagg gctacgccaa gggcgtgctg acacagaaac
     6481 tgggaccttg gagaagacct gtggcttatc tcagcaagaa gctggacccc gtggccgccg
     6541 gatggcctcc atgtctgagg atggtcgccg ctatcgccgt gctgaccaag gacgccggca
     6601 aactgaccat gggacaacct ctggtgatcc tggcccctca cgccgtcgag gccctggtga
     6661 agcagcctcc tgacagatgg ctgagcaacg ccagaatgac ccactaccag gccctgctgc
     6721 tggataccga tagagtgcag ttcggccctg tggtggctct gaatcctgcc accctgctcc
     6781 cacttcccga ggaaggcctg cagcacaact gcctggacat cctggcagaa gcccacggca
     6841 caagacctga cttaacagac cagcctctgc ctgatgccga ccacacctgg tataccgacg
     6901 gcagcagcct gctgcaggag ggccaaagaa aggccggagc cgccgtgaca acagaaaccg
     6961 aggtgatctg ggccaaggcc ctccctgctg gcaccagcgc ccaaagagcc gagctgattg
     7021 ccctcaccca ggccctgaag atggccgaag gcaagaaact gaatgtgtac actgactcta
     7081 gatacgcctt tgccacagcc cacatccacg gcgagatcta cagacggaga ggatggctaa
     7141 cctctgaggg caaggaaatc aaaaacaagg atgagattct ggccctgctg aaggcactgt
     7201 ttctgcccaa gagactgagc atcatccact gccccggcca ccagaaggga cacagcgccg
     7261 aggcccgggg caatagaatg gccgatcagg ccgctcggaa ggccgccatc accgaaacac
     7321 ccgacaccag cacacttctg atcgagaact ccagcccctc tggcggctca aaaagaaccg
     7381 ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc atcatcacca
     7441 tcaccattga ggcggccgca ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     7501 tttttgtgtc tctcattaat taaaataaaa tatctttatt ttcattacat ctgtgtgttg
     7561 gttttttgtg tggctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc
     7621 ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtctag aaaaaaaaaa
     7681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agggtcttca tcatcgcctg
     7741 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8581 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     8641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9901 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9961 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
