LOCUS       Exported               12808 bp ds-DNA     circular SYN 05-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Rasmus O. Bak
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 5, 2022 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     10..29
                     /label=KAS59
     primer_bind     78..96
                     /label=KAS18
     primer_bind     79..110
                     /label=KAS310
     primer_bind     79..100
                     /label=AKH5220
     primer_bind     79..98
                     /label=KAS1
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     94..136
                     /label=LR139
     primer_bind     95..120
                     /label=LR1
     primer_bind     95..120
                     /label=LR6
     primer_bind     97..124
                     /label=LR171
     primer_bind     98..135
                     /label=LR148
     primer_bind     98..122
                     /label=AKH5186
     primer_bind     complement(120..140)
                     /label=KAS197
     primer_bind     158..180
                     /label=KAS185
     primer_bind     complement(230..248)
                     /label=KAS56
     primer_bind     complement(338..357)
                     /label=KAS326
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     585..609
                     /label=LR47
     LTR             680..860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..777
                     /label=R region of 5' LTR
                     /note="Oriinal location: 680..777"
     misc_feature    778..860
                     /label=U5 region of 5' LTR
                     /note="Oriinal location: 778..860"
     misc_feature    904..1029
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     993..1012
                     /label=EAT660
     misc_feature    1013..1015
                     /label=gag start codon
                     /note="Oriinal location: 1013..1015"
     misc_feature    1076..1078
                     /label=gag stop codon
                     /note="Oriinal location: 1076..1078"
     primer_bind     complement(1305..1361)
                     /label=EAT326
     primer_bind     1305..1361
                     /label=EAT331
     primer_bind     1343..1388
                     /label=EAT488
     misc_feature    1522..1755
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     primer_bind     1596..1615
                     /label=AKH5174 pc
     CDS             1939..1983
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2101..2130
                     /label=Splice Acceptor
                     /note="Oriinal location: 2101..2130"
     primer_bind     complement(2143..2157)
                     /label=EAT489
     primer_bind     2167..2189
                     /label=LR144
     primer_bind     2225..2244
                     /label=AKH5219 pc
     misc_feature    2250..2367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2270..2287
                     /label=EAT287
     primer_bind     2270..2287
                     /label=EAT285
     primer_bind     2276..2295
                     /label=LR36
     primer_bind     complement(2289..2332)
                     /label=EAT22
     primer_bind     complement(2308..2327)
                     /label=EAT57
     primer_bind     complement(2346..2365)
                     /label=EAT61
     primer_bind     2388..2408
                     /label=AKH5029
     primer_bind     2388..2408
                     /label=AKH5057
     primer_bind     2396..2421
                     /label=AKH5112
     primer_bind     2398..2416
                     /label=LR190
     primer_bind     2402..2425
                     /label=KAS385
     primer_bind     2402..2421
                     /label=AKH5117
     primer_bind     2402..2419
                     /label=KAS231
     primer_bind     2402..2419
                     /label=KAS253
     promoter        2406..2905
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     2517..2527
                     /label=AKH5030
     primer_bind     complement(2524..2543)
                     /label=AKH5030
     primer_bind     2681..2721
                     /label=KAS256
     primer_bind     2836..2855
                     /label=KAS45
     primer_bind     2892..2941
                     /label=NEB_LentiPE_Frag1.FOR
     misc_feature    2917..2927
                     /label=Kozak
     CDS             2926..2979
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             2980..7080
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     3259..3301
                     /label=Cas9_Frag2.v2.TOP
     primer_bind     7070..7087
                     /label=ZG508
     CDS             7081..7179
                     /codon_start=1
                     /label=Linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGSS"
     CDS             7180..8589
                     /codon_start=1
                     /label=MLV-RT (truncated)
                     /translation="LGSTWLSDFPQAWAETGGMGLAVRQAPLIIPLKATSTPVSIKQYP
                     MSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKPGTNDYRPVQDLREVNKRVED
                     IHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPTSQPLFAFEWRDPEMGISGQL
                     TWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQYVDDLLLAATSELDCQQGTRA
                     LLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLTEARKETVMGQPTPKTPRQLR
                     EFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQQKAYQEIKQALLTAPALGLP
                     DLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKLDPVAAGWPPCLRMVAAIAVL
                     TKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMTHYQALLLDTDRVQFGPVVAL
                     NPATLLPLPEEG"
     primer_bind     7430..7447
                     /label=ZG782
     primer_bind     8130..8147
                     /label=ZG513
     primer_bind     complement(8139..8159)
                     /label=ZG512
     primer_bind     complement(8628..8678)
                     /label=NEB_LentiPE_Frag1.REV
     primer_bind     8628..8678
                     /label=NEB_LentiPE_Frag2.FOR
     CDS             8632..8652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8653..8676
                     /label=FLAG
     misc_feature    8677..8742
                     /label=P2A
     CDS             8743..9141
                     /label=BLAST
                     /label=Blast resistance
     primer_bind     9079..9090
                     /label=NEB_LentiPE_Frag1.FOR
     primer_bind     9122..9176
                     /label=NEB_LentiPE_Frag3.FOR
     primer_bind     complement(9122..9148)
                     /label=NEB_LentiPE_Frag2.REV
     misc_feature    9154..9402
                     /label=W3
     primer_bind     9378..9433
                     /label=NEB_LentiPE_Frag4.FOR
     primer_bind     complement(9378..9431)
                     /label=NEB_LentiPE_Frag3.REV
     LTR             9432..9665
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9697..9921
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9898..9950)
                     /label=NEB_LentiPE_Frag4.REV
     promoter        complement(9951..9969)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9979..9995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10140..10595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10230..10250)
                     /label=AKH5255
     primer_bind     complement(10381..10400)
                     /label=KAS143
     promoter        10624..10728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10729..11589
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10829..10848)
                     /label=AKH5032 pc
     rep_origin      11760..12348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12501..12557
                     /label=EAT327
     primer_bind     complement(12501..12557)
                     /label=EAT332
     primer_bind     complement(12526..12570)
                     /label=KAS385
     primer_bind     12618..12645
                     /label=KAS17
     protein_bind    12636..12657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     12650..12672
                     /label=ABR78
     promoter        12672..12702
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12710..12726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12734..12750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12771..12790
                     /label=KAS73
     promoter        12771..12789
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc
      721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      841 agtgtggaaa atctctagca gtggcgcccg aacagggacc tgaaagcgaa agggaaacca
      901 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      961 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1021 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1081 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1141 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1201 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1261 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1321 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1381 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1441 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1501 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1561 cactatgggc gcagcctcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1621 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1681 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1741 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1801 gccttggaat gctagttgga gtaataaatc tctggaacag attggaatca cacgacctgg
     1861 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1921 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1981 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     2041 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     2101 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2161 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2221 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     2281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2341 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga cgatggtcga
     2401 gtaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2461 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2521 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2581 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     2641 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     2701 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     2761 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     2821 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     2881 ctcttcctca tctccgggcc tttcgacctc tagcgagcca ccatgaaacg gacagccgac
     2941 ggaagcgagt tcgagtcacc aaagaagaag cggaaagtcg acaagaagta cagcatcggc
     3001 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
     3061 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
     3121 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
     3181 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
     3241 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
     3301 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
     3361 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
     3421 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
     3481 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
     3541 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
     3601 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
     3661 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg
     3721 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag
     3781 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac
     3841 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg
     3901 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac
     3961 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa
     4021 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc
     4081 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa
     4141 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc
     4201 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat
     4261 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc
     4321 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag
     4381 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc
     4441 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg
     4501 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa
     4561 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc
     4621 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac
     4681 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac
     4741 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac
     4801 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac
     4861 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg
     4921 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac
     4981 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc
     5041 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc
     5101 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc
     5161 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg
     5221 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag
     5281 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc
     5341 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac
     5401 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg
     5461 gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag ctttctgaag
     5521 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac
     5581 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac
     5641 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg
     5701 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca
     5761 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag
     5821 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag
     5881 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac
     5941 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc
     6001 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa
     6061 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc
     6121 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa
     6181 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc
     6241 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag
     6301 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct
     6361 aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa
     6421 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc
     6481 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa
     6541 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac
     6601 ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg
     6661 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc
     6721 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag
     6781 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac
     6841 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat
     6901 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac
     6961 accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc
     7021 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac
     7081 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca
     7141 acaccagaga gcagtggcgg cagcagcggc ggcagcagcc tgggttctac atggctgagc
     7201 gacttccctc aggcctgggc tgaaaccggc ggcatgggcc tggctgtgcg gcaggctcct
     7261 ctgatcatcc ctctgaaggc cacctccacc cctgtgtcca tcaagcagta ccccatgagc
     7321 caggaggcca gactgggcat caagccccac atccagcggc tgctcgacca gggaatcctg
     7381 gtcccctgcc agagcccctg gaacacccca cttttacctg tgaaaaagcc cggcactaac
     7441 gactaccggc ctgtgcagga cctgagagaa gtgaacaagc gggttgagga catccaccct
     7501 acagtgccta acccctacaa cctgctgtct ggcctgccac cgagccacca gtggtacacc
     7561 gtgctggacc tgaaagacgc cttcttctgt ctgagactcc atcctaccag ccagccactg
     7621 ttcgccttcg agtggcggga ccctgagatg ggcatcagcg gccagctgac atggacccgg
     7681 ctgccacagg gcttcaagaa cagccctaca ctgttcaacg aggccctgca cagagatctg
     7741 gctgacttca gaatccagca tcctgacctg atcctgctgc agtacgtgga cgacttgctg
     7801 cttgccgcta catctgaact ggattgccag caaggcacca gagctctgct ccagaccctg
     7861 ggcaacctgg ggtatagggc cagcgccaag aaggcccaga tctgccagaa gcaagtgaag
     7921 tacctgggat acctgctgaa ggaaggccag agatggctga ccgaggcgcg gaaggaaaca
     7981 gtgatgggcc agcctacccc taagaccccc agacagctgc gagaattcct gggcaaggct
     8041 ggcttttgca gactgttcat ccccggcttc gctgagatgg ccgctcctct gtacccactg
     8101 accaagcccg gtaccctgtt caattggggc cccgatcagc agaaagccta ccaggagatt
     8161 aagcaggccc tgctgaccgc ccctgccctc gggctgcctg atctgacaaa acctttcgag
     8221 ctgtttgtcg acgagaagca gggctacgcc aagggcgtgc tgacacagaa actgggacct
     8281 tggagaagac ctgtggctta tctcagcaag aagctggacc ccgtggccgc cggatggcct
     8341 ccatgtctga ggatggtcgc cgctatcgcc gtgctgacca aggacgccgg caaactgacc
     8401 atgggacaac ctctggtgat cctggcccct cacgccgtcg aggccctggt gaagcagcct
     8461 cctgacagat ggctgagcaa cgccagaatg acccactacc aggccctgct gctggatacc
     8521 gatagagtgc agttcggccc tgtggtggct ctgaatcctg ccaccctgct cccacttccc
     8581 gaggaaggct ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
     8641 aagaggaaag tcgattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
     8701 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct
     8761 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     8821 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     8881 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     8941 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     9001 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     9061 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     9121 ggttatgtgt gggagggcta agaattccga tctgataatc aacctctgga ttacaaaatt
     9181 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     9241 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     9301 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9361 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtagccact ttttaaaaga
     9421 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     9481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9661 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     9721 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     9781 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     9841 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     9901 gggatgcggt gggctctatg ggcttttgcg tcgagacgta cccaattcgc cctatagtga
     9961 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    10021 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    10081 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
    10141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
    10201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
    10261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
    10381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    10501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10561 cgcgaatttt aacaaaatat taacgtttac aatttcccag gtggcacttt tcggggaaat
    10621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    10741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    10801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    10981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    11041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    11101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    11161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    11281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    11341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    11401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    11461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    11521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    11581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    11641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    11701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11881 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    11941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    12001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    12061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    12121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    12541 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    12601 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    12661 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    12721 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    12781 cactaaaggg aacaaaagct ggagctgc
//
