LOCUS       pJAB2098_proUBQ10::PIP2 7686 bp ds-DNA     circular     01-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        2556..3342
                     /label="35S Promter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2629..2629
                     /label="G->C mutation to remove Bsa1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3310..3330
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    3328..3333
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3343..3363
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    3365..3371
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    3372..3372
                     /label="A->T mutation to remove ATG in PhlFO"
                     /ApEinfo_revcolor=#00572c
                     /ApEinfo_fwdcolor=#00572c
     misc_feature    3373..3401
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     promoter        3402..3404
                     /label="35S Promter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3405..3405
                     /label="A->T mutation to remove ATG in PhlFO"
                     /ApEinfo_revcolor=#00572c
                     /ApEinfo_fwdcolor=#00572c
     misc_feature    3406..3434
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     promoter        3435..3451
                     /label="35S Promter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3452..3455
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3456..3656
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3657..3845
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3846..4364
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3995..3995
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4365..4368
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4369..4619
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4620..4623
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4626..4631)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4690..4709)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4727..4747)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5084..5132
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5181..5862
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5881..6666)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6667..6878)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7118..7118
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7119..7671
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgGGTCC CCAGATTTGC CTTTTCAATT
     2581 TCAGAAAGAA TGCTAACCCA CAGATGGGTA GAGAGGCTTA CGCAGCAGCT CTCATCAAGA
     2641 CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG
     2701 CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA
     2761 TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA
     2821 TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA
     2881 TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC
     2941 TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG
     3001 TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC
     3061 AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA
     3121 TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA
     3181 AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA
     3241 GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG
     3301 ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCTTCTATCG ATCTATAGAT
     3361 AATCTATATA ATTGATACGA AACGTACCGT ATCGTTAAGG TGGATTGATA CGAAACGTAC
     3421 CGTATCGTTA AGGTACACGG GGGACTCTAG ACACCATGGT GAGTAAAGGA GAAGAACTTT
     3481 TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG CACAAATTTT
     3541 CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT AAATTTATTT
     3601 GCACTACTGG AAAACTACCT GTTCCATGGC CAACCCTGGT CACCACCCTG ACCTACgtaa
     3661 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     3721 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     3781 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg
     3841 tgcagGGCGT GCAGTGCTTC TCCCGTTACC CTGATCATAT GAAGCGGCAC GACTTCTTCA
     3901 AGAGCGCCAT GCCTGAGGGA TACGTGCAGG AGAGGACCAT CTTCTTCAAG GACGACGGGA
     3961 ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AGGGTGACAC CCTCGTCAAC AGGATCGAGC
     4021 TTAAGGGAAT CGATTTCAAG GAGGACGGAA ACATCCTCGG CCACAAGTTG GAATACAACT
     4081 ACAACTCCCA CAACGTATAC ATCATGGCCG ACAAGCAAAA GAACGGCATC AAAGCCAACT
     4141 TCAAGACCCG CCACAACATC GAGGACGGCG GCGTGCAACT CGCTGATCAT TATCAACAAA
     4201 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT
     4261 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGATCACAT GGTCCTTCTT GAGTTTGTAA
     4321 CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA ATAAtgtcaa gccattctct
     4381 cgcagatgat gttcactttg tgttttactt cctttatgca ttcacagcaa taaaagaaag
     4441 aaatctccat cgcttttggt tttcttctct gtcttaagtt agtcgttttc gtgtctaatc
     4501 tattacttat cattgtaata gactcttctt ctattgagat ttgaatataa actaaaacac
     4561 attccatttt actgtgttct caacattcag aatgcaaacg gactaaccgt agtgactcgg
     4621 cttgaGTCTT Cgggctagag cggactagtt ctagagcggc cgccaccgcg gtggagctcc
     4681 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     4741 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4801 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4861 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4921 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4981 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5041 tccacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
     5101 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
     5161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5641 acagtatttg gtatctgTgc tctgctgaag ccagttacct tcggaagaag agttggtagc
     5701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgaattcttg ttagacatta
     5881 tttgccgact accttggtga tctcgccttt cacgtagtga acaaattctt ccaactgatc
     5941 tgcgcgcgag gccaagcgat cttcttgtcc aagataagcc tgcctagctt caagtatgac
     6001 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     6061 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     6121 atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     6181 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     6241 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     6301 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     6361 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     6421 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     6481 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     6541 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     6601 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     6661 cctcatgatg tttaactcct gaattaagcc gcgccgcgaa gcggtgtCgg cttgaatgaa
     6721 ttgttaggcg tcatcctgtg ctcccgagaa ccagtaccag tacatcgctg tttcgttcga
     6781 gacttgaggt ctagttttat acgtgaacag gtcaatgccg ccgagagtaa agccacaTtt
     6841 tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgCC ATGATGATAT ATTTTTATCT
     6901 TGTGCAATGT AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTGTTGAAT AAATCGAACT
     6961 TTTGCTGAGT TGAAGGATCA GATCACGCAT CTTCCCGACA ACGCAGACCG TTCCGTGGCA
     7021 AAGCAAAAGT TCAAAATCAC CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC
     7081 TCCCTCACTT TCTGGCTGGA TGATGGGGCG ATTCAGGAGA TCCCCATCCA ACAGCCCGCC
     7141 GTCGAGCGGG CTTTTTTATC CCCGGAAGCC TGTGGATAGA GGGTAGTTAT CCACGTGAAA
     7201 CCGCTAATGC CCCGCAAAGC CTTGATTCAC GGGGCTTTCC GGCCCGCTCC AAAAACTATC
     7261 CACGTGAAAT CGCTAATCAG GGTACGTGAA ATCGCTAATC GGAGTACGTG AAATCGCTAA
     7321 TAAGGTCACG TGAAATCGCT AATCAAAAAG GCACGTGAGA ACGCTAATAG CCCTTTCAGA
     7381 TCAACAGCTT GCAAACACCC CTCGCTCCGG CAAGTAGTTA CAGCAAGTAG TATGTTCAAT
     7441 TAGCTTTTCA ATTATGAATA TATATATCAA TTATTGGTCG CCCTTGGCTT GTGGACAATG
     7501 CGCTACGCGC ACCGGCTCCG CCCGTGGACA ACCGCAAGCG GTTGCCCACC GTCGAGCGCC
     7561 TTTGCCCACA ACCCGGCGGC CGGCCGCAAC AGATCGTTTT ATAAATTTTT TTTTTTGAAA
     7621 AAGAAAAAGC CCGAAAGGCG GCAACCTCTC GGGCTTCTGG ATTTCCGATC Cccggaatta
     7681 gactct
//