LOCUS       pJAB2121_control        4464 bp ds-DNA     circular     07-JUL-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..49
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    98..779
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(798..1583)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1584..1795)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2036..2036
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2037..2589
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2669..2693
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(2744..3027)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(3035..3586)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3308..3308)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(3603..3895)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3933..3938
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3941..3944
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3947..3950
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3952..3957)
                     /label="Bsa1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3988..3993
                     /label="Bsa1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3995..3998
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4001..4004
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4007..4012)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4071..4090)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4108..4128)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt
       61 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      121 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      181 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      241 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      301 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      361 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      421 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      481 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      541 acggctacac tagaagaaca gtatttggta tctgTgctct gctgaagcca gttaccttcg
      601 gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      661 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      721 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      781 attcttgtta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca
      841 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc
      901 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
      961 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     1021 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt
     1081 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     1141 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     1201 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     1261 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     1321 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     1381 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     1441 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     1501 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     1561 tcggcgatca ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg
     1621 gtgtCggctt gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac
     1681 atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg
     1741 agagtaaagc cacaTtttgc gtacaaattg caggcaggta cattgttcgt ttgtgCCATG
     1801 ATGATATATT TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA ACGTGGCTTT
     1861 GTTGAATAAA TCGAACTTTT GCTGAGTTGA AGGATCAGAT CACGCATCTT CCCGACAACG
     1921 CAGACCGTTC CGTGGCAAAG CAAAAGTTCA AAATCACCAA CTGGTCCACC TACAACAAAG
     1981 CTCTCATCAA CCGTGGCTCC CTCACTTTCT GGCTGGATGA TGGGGCGATT CAGGCAGATC
     2041 CCCATCCAAC AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG
     2101 GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG
     2161 CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG
     2221 AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC
     2281 GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA
     2341 GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC
     2401 CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT
     2461 TGCCCACCGT CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT
     2521 AAATTTTTTT TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT
     2581 TTCCGATCCc cggaattaga ctctggctgc ctgtatcgag tggtgatttt gtgccgagct
     2641 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     2701 cgcttagaca acttaataac acattgcgga cgtttttaat gtaatcagct tgcatgccgg
     2761 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     2821 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     2881 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     2941 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     3001 aatgtttgaa cgatctgctt gactctaggg gtcatcagat ttcggtgacg ggcaggaccg
     3061 gacggggcgg caccggcagg ctgaagtcca gctgccagaa acccacgtca tgccagttcc
     3121 cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc gcctcgtgca
     3181 tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg aagccctgtg
     3241 cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc
     3301 ggggggaCac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt gccttccagg
     3361 gacccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc cagggatagc
     3421 gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga
     3481 agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg
     3541 cctcggtggc acggcggatg tcggccgggc gtcgttctgg gctcatggta gatcccctcg
     3601 atcgagttga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     3661 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     3721 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     3781 tcagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     3841 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc gataacatgt
     3901 cgactatcgg acgagctcaa agtttaacgT gtGAAGACgt gcttctaatg cGAGACCACC
     3961 CGGCGCTAAC GCACTCAAAG CCGGGACGGT CTCtgcttga tactacGTCT TCgggctaga
     4021 gcggactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     4081 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4441 acgcaggaaa gaacatgaag gcct
//