LOCUS       pJAB2144_proUBQ10::PIP2 7137 bp ds-DNA     circular     29-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2583..2603
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2607..2627
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2631..2651
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2655..2675
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2679..2699
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2703..2723
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2748..2751
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2752..2781
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2782..2785
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2786..2791
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2786..2868
                     /label="min35S"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2793..2797
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2821..2827
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    2851..2851
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2869..2872
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2873..2873
                     /label="A->T mutation to remove ATG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2873..2902
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2903..2906
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2907..3107
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3108..3296
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3297..3815
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3446..3446
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3816..3819
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3820..4070
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4071..4074
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4077..4082)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4141..4160)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4178..4198)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4535..4583
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4632..5313
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5332..6117)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6118..6329)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6569..6569
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6570..7122
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgATAGA AAAGTTGGCT GCGGCCGCGA
     2581 ATTTCTATCG ATCTATAGAT AATGATTTCT ATCGATCTAT AGATAATCAT TTCTATCGAT
     2641 CTATAGATAA TCATTTCTAT CGATCTATAG ATAATGTTTT CTATCGATCT ATAGATAATA
     2701 GTTTCTATCG ATCTATAGAT AATCGGGTCT AAGCTAGCTA GGCCACCTAG CATGATACGA
     2761 AACGTACCGT ATCGTTAAGG TAGGTCACAA TcCCACTatc ctttcgcaag acccttcctc
     2821 TATATAAgga agttcatttc atttggagag Aacacggggg actctagaGT AATTGATACG
     2881 AAACGTACCG TATCGTTAAG GTCACCATGG TGAGTAAAGG AGAAGAACTT TTCACTGGAG
     2941 TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG
     3001 GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG
     3061 GAAAACTACC TGTTCCATGG CCAACCCTGG TCACCACCCT GACCTACgta agtttctgct
     3121 tctacctttg atatatatat aataattatc attaattagt agtaatataa tatttcaaat
     3181 atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag tttataagtg
     3241 tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat gtgcagGGCG
     3301 TGCAGTGCTT CTCCCGTTAC CCTGATCATA TGAAGCGGCA CGACTTCTTC AAGAGCGCCA
     3361 TGCCTGAGGG ATACGTGCAG GAGAGGACCA TCTTCTTCAA GGACGACGGG AACTACAAGA
     3421 CACGTGCTGA AGTCAAGTTT GAGGGTGACA CCCTCGTCAA CAGGATCGAG CTTAAGGGAA
     3481 TCGATTTCAA GGAGGACGGA AACATCCTCG GCCACAAGTT GGAATACAAC TACAACTCCC
     3541 ACAACGTATA CATCATGGCC GACAAGCAAA AGAACGGCAT CAAAGCCAAC TTCAAGACCC
     3601 GCCACAACAT CGAGGACGGC GGCGTGCAAC TCGCTGATCA TTATCAACAA AATACTCCAA
     3661 TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA TCTGCCCTTT
     3721 CGAAAGATCC CAACGAAAAG AGAGATCACA TGGTCCTTCT TGAGTTTGTA ACAGCTGCTG
     3781 GGATTACACA TGGCATGGAT GAACTATACA AATAAtgtca agccattctc tcgcagatga
     3841 tgttcacttt gtgttttact tcctttatgc attcacagca ataaaagaaa gaaatctcca
     3901 tcgcttttgg ttttcttctc tgtcttaagt tagtcgtttt cgtgtctaat ctattactta
     3961 tcattgtaat agactcttct tctattgaga tttgaatata aactaaaaca cattccattt
     4021 tactgtgttc tcaacattca gaatgcaaac ggactaaccg tagtgactcg gcttgaGTCT
     4081 TCgggctaga gcggactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     4141 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     4201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4501 tcaggggata acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact
     4561 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     4621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5101 ggtatctgTg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     5161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5281 aacgaaaact cacgttaagg gattttggtc atgaattctt gttagacatt atttgccgac
     5341 taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat ctgcgcgcga
     5401 ggccaagcga tcttcttgtc caagataagc ctgcctagct tcaagtatga cgggctgata
     5461 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     5521 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     5581 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     5641 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     5701 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     5761 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     5821 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     5881 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     5941 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     6001 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     6061 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
     6121 gtttaactcc tgaattaagc cgcgccgcga agcggtgtCg gcttgaatga attgttaggc
     6181 gtcatcctgt gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg
     6241 tctagtttta tacgtgaaca ggtcaatgcc gccgagagta aagccacaTt ttgcgtacaa
     6301 attgcaggca ggtacattgt tcgtttgtgC CATGATGATA TATTTTTATC TTGTGCAATG
     6361 TAACATCAGA GATTTTGAGA CACAACGTGG CTTTGTTGAA TAAATCGAAC TTTTGCTGAG
     6421 TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC GTTCCGTGGC AAAGCAAAAG
     6481 TTCAAAATCA CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG CTCCCTCACT
     6541 TTCTGGCTGG ATGATGGGGC GATTCAGGAG ATCCCCATCC AACAGCCCGC CGTCGAGCGG
     6601 GCTTTTTTAT CCCCGGAAGC CTGTGGATAG AGGGTAGTTA TCCACGTGAA ACCGCTAATG
     6661 CCCCGCAAAG CCTTGATTCA CGGGGCTTTC CGGCCCGCTC CAAAAACTAT CCACGTGAAA
     6721 TCGCTAATCA GGGTACGTGA AATCGCTAAT CGGAGTACGT GAAATCGCTA ATAAGGTCAC
     6781 GTGAAATCGC TAATCAAAAA GGCACGTGAG AACGCTAATA GCCCTTTCAG ATCAACAGCT
     6841 TGCAAACACC CCTCGCTCCG GCAAGTAGTT ACAGCAAGTA GTATGTTCAA TTAGCTTTTC
     6901 AATTATGAAT ATATATATCA ATTATTGGTC GCCCTTGGCT TGTGGACAAT GCGCTACGCG
     6961 CACCGGCTCC GCCCGTGGAC AACCGCAAGC GGTTGCCCAC CGTCGAGCGC CTTTGCCCAC
     7021 AACCCGGCGG CCGGCCGCAA CAGATCGTTT TATAAATTTT TTTTTTTGAA AAAGAAAAAG
     7081 CCCGAAAGGC GGCAACCTCT CGGGCTTCTG GATTTCCGAT CCccggaatt agactct
//