LOCUS       pJAB2177_proUBQ10::PIP2 7065 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2583..2603
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2607..2627
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2631..2651
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2655..2675
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2679..2699
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2703..2723
                     /label="AmtRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2744..2747
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2748..2753
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2748..2830
                     /label="min35S promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2755..2759
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2783..2789
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    2813..2813
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2831..2834
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2835..3035
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3036..3224
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3225..3743
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3374..3374
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3744..3747
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3748..3998
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3999..4002
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4005..4010)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4069..4088)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4106..4126)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4463..4511
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4560..5241
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5260..6045)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6046..6257)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6497..6497
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6498..7050
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgATAGA AAAGTTGGCT GCGGCCGCGA
     2581 ATTTCTATCG ATCTATAGAT AATGATTTCT ATCGATCTAT AGATAATCAT TTCTATCGAT
     2641 CTATAGATAA TCATTTCTAT CGATCTATAG ATAATGTTTT CTATCGATCT ATAGATAATA
     2701 GTTTCTATCG ATCTATAGAT AATCGGGTCT AAGCTAGCTA GGCCACCCAC AATcCCACTa
     2761 tcctttcgca agacccttcc tcTATATAAg gaagttcatt tcatttggag agAacacggg
     2821 ggactctaga CACCATGGTG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC
     2881 TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG
     2941 GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG
     3001 TTCCATGGCC AACCCTGGTC ACCACCCTGA CCTACgtaag tttctgcttc tacctttgat
     3061 atatatataa taattatcat taattagtag taatataata tttcaaatat ttttttcaaa
     3121 ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatattttaa
     3181 tttataactt ttctaatata tgaccaaaat ttgttgatgt gcagGGCGTG CAGTGCTTCT
     3241 CCCGTTACCC TGATCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCTGAGGGAT
     3301 ACGTGCAGGA GAGGACCATC TTCTTCAAGG ACGACGGGAA CTACAAGACA CGTGCTGAAG
     3361 TCAAGTTTGA GGGTGACACC CTCGTCAACA GGATCGAGCT TAAGGGAATC GATTTCAAGG
     3421 AGGACGGAAA CATCCTCGGC CACAAGTTGG AATACAACTA CAACTCCCAC AACGTATACA
     3481 TCATGGCCGA CAAGCAAAAG AACGGCATCA AAGCCAACTT CAAGACCCGC CACAACATCG
     3541 AGGACGGCGG CGTGCAACTC GCTGATCATT ATCAACAAAA TACTCCAATT GGCGATGGCC
     3601 CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG AAAGATCCCA
     3661 ACGAAAAGAG AGATCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG ATTACACATG
     3721 GCATGGATGA ACTATACAAA TAAtgtcaag ccattctctc gcagatgatg ttcactttgt
     3781 gttttacttc ctttatgcat tcacagcaat aaaagaaaga aatctccatc gcttttggtt
     3841 ttcttctctg tcttaagtta gtcgttttcg tgtctaatct attacttatc attgtaatag
     3901 actcttcttc tattgagatt tgaatataaa ctaaaacaca ttccatttta ctgtgttctc
     3961 aacattcaga atgcaaacgg actaaccgta gtgactcggc ttgaGTCTTC gggctagagc
     4021 ggactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     4081 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4141 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4201 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4261 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4321 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4381 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4441 gcaggaaaga acatgaaggc cttgacagga tatattggcg ggtaaactaa gtcgctgtat
     4501 gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4561 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4621 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4681 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4741 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4801 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4921 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4981 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgTgct
     5041 ctgctgaagc cagttacctt cggaagaaga gttggtagct cttgatccgg caaacaaacc
     5101 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5221 cgttaaggga ttttggtcat gaattcttgt tagacattat ttgccgacta ccttggtgat
     5281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     5341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
     5401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5581 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5641 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5701 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5761 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5821 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5881 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5941 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     6001 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
     6061 aattaagccg cgccgcgaag cggtgtCggc ttgaatgaat tgttaggcgt catcctgtgc
     6121 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
     6181 cgtgaacagg tcaatgccgc cgagagtaaa gccacaTttt gcgtacaaat tgcaggcagg
     6241 tacattgttc gtttgtgCCA TGATGATATA TTTTTATCTT GTGCAATGTA ACATCAGAGA
     6301 TTTTGAGACA CAACGTGGCT TTGTTGAATA AATCGAACTT TTGCTGAGTT GAAGGATCAG
     6361 ATCACGCATC TTCCCGACAA CGCAGACCGT TCCGTGGCAA AGCAAAAGTT CAAAATCACC
     6421 AACTGGTCCA CCTACAACAA AGCTCTCATC AACCGTGGCT CCCTCACTTT CTGGCTGGAT
     6481 GATGGGGCGA TTCAGGAGAT CCCCATCCAA CAGCCCGCCG TCGAGCGGGC TTTTTTATCC
     6541 CCGGAAGCCT GTGGATAGAG GGTAGTTATC CACGTGAAAC CGCTAATGCC CCGCAAAGCC
     6601 TTGATTCACG GGGCTTTCCG GCCCGCTCCA AAAACTATCC ACGTGAAATC GCTAATCAGG
     6661 GTACGTGAAA TCGCTAATCG GAGTACGTGA AATCGCTAAT AAGGTCACGT GAAATCGCTA
     6721 ATCAAAAAGG CACGTGAGAA CGCTAATAGC CCTTTCAGAT CAACAGCTTG CAAACACCCC
     6781 TCGCTCCGGC AAGTAGTTAC AGCAAGTAGT ATGTTCAATT AGCTTTTCAA TTATGAATAT
     6841 ATATATCAAT TATTGGTCGC CCTTGGCTTG TGGACAATGC GCTACGCGCA CCGGCTCCGC
     6901 CCGTGGACAA CCGCAAGCGG TTGCCCACCG TCGAGCGCCT TTGCCCACAA CCCGGCGGCC
     6961 GGCCGCAACA GATCGTTTTA TAAATTTTTT TTTTTGAAAA AGAAAAAGCC CGAAAGGCGG
     7021 CAACCTCTCG GGCTTCTGGA TTTCCGATCC ccggaattag actct
//