LOCUS       pJAB2180_proG1090::TetR 5778 bp ds-DNA     circular     29-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2150
                     /label="TetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2151..2384
                     /label="VP16 Hasseloff"
                     /ApEinfo_revcolor=#6e42fa
                     /ApEinfo_fwdcolor=#6e42fa
     misc_feature    2385..2405
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2406..2408
                     /label="Activator stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2409..2412
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2416..2640
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2641..2644
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2647..2650
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2653..2658)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2717..2736)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2754..2774)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3111..3159
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3208..3889
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3908..4693)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4694..4905)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5146..5146
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5147..5699
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGAGCCGGCT GGACAAGAGC AAAGTGATCA
     1561 ACAGCGCCCT GGAACTGCTC AACGAAGTGG GCATCGAGGG CCTGACCACC CGGAAGCTGG
     1621 CTCAGAAACT GGGCGTGGAA CAGCCCACCC TGTACTGGCA CGTGAAGAAC AAGAGAGCCC
     1681 TGCTGGACGC CCTGGCCATC GAGATGCTGG ACCGGCACCA CACCCACTTT TGCCCCCTGG
     1741 AAGGCGAGAG CTGGCAGGAT TTCCTGCGGA ACAACGCCAA GAGCTTCAGA TGCGCTCTGC
     1801 TGTCCCACCG GGATGGCGCC AAAGTGCACC TGGGCACCAG ACCTACCGAG AAGCAGTACG
     1861 AGACACTGGA AAACCAGCTG GCCTTCCTGT GCCAGCAGGG CTTCAGCCTG GAAAATGCCC
     1921 TGTACGCCCT GAGCGCCGTG GGCCACTTTA CACTGGGCTG CGTGCTGGAA GATCAGGAAC
     1981 ACCAGGTCGC CAAAGAGGAA AGAGAGACAC CCACCACCGA CAGCATGCCC CCTCTGCTGA
     2041 GACAGGCCAT TGAGCTGTTC GATCATCAGG GCGCCGAGCC CGCCTTTCTG TTCGGCCTCG
     2101 AGCTGATCAT CTGTGGCCTC GAAAAACAGC TGAAGTGCGA GTCCGGCAGC GCCCCCCCGA
     2161 CCGACGTCAG CCTGGGGGAC GAGCTCCACT TAGACGGCGA GGACGTGGCG ATGGCGCATG
     2221 CCGACGCGCT AGACGATTTC GATCTGGACA TGTTGGGGGA CGGGGATTCC CCGGGGCCGG
     2281 GATTTACCCC CCACGACTCC GCCCCCTACG GCGCTCTGGA TACGGCCGAC TTCGAGTTTG
     2341 AGCAGATGTT TACCGATGCC CTTGGAATTG ACGAGTACGG TGGGccgaag aagaagagga
     2401 aggttTAGtg tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat
     2461 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg
     2521 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     2581 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgat
     2641 gcttgatact acGTCTTCgg gctagagcgg actagttcta gagcggccgc caccgcggtg
     2701 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     2761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3061 acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata
     3121 tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg
     3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3661 tagaagaaca gtatttggta tctgTgctct gctgaagcca gttaccttcg gaagaagagt
     3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga attcttgtta
     3901 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca
     3961 actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa
     4021 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     4081 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     4141 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     4201 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     4261 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     4321 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     4381 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     4441 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     4501 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     4561 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     4621 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     4681 ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtCggctt
     4741 gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt
     4801 cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc
     4861 cacaTtttgc gtacaaattg caggcaggta cattgttcgt ttgtgCCATG ATGATATATT
     4921 TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA ACGTGGCTTT GTTGAATAAA
     4981 TCGAACTTTT GCTGAGTTGA AGGATCAGAT CACGCATCTT CCCGACAACG CAGACCGTTC
     5041 CGTGGCAAAG CAAAAGTTCA AAATCACCAA CTGGTCCACC TACAACAAAG CTCTCATCAA
     5101 CCGTGGCTCC CTCACTTTCT GGCTGGATGA TGGGGCGATT CAGGCAGATC CCCATCCAAC
     5161 AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC
     5221 ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA
     5281 AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA
     5341 ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC
     5401 CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA
     5461 TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT
     5521 GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT
     5581 CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT AAATTTTTTT
     5641 TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT TTCCGATCCc
     5701 cggaattaga ctctggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     5761 gagctgttgg ctggctgg
//