LOCUS       pJAB2185_proUBQ10::PIP2 7035 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2582..2598
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2601..2617
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2620..2636
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2639..2655
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2658..2674
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2677..2693
                     /label="McbRO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2701..2701
                     /label="C ->A mutation to remove Bsa1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2714..2717
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2718..2723
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2718..2800
                     /label="min35S promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2725..2729
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2753..2759
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    2783..2783
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2801..2804
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2805..3005
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3006..3194
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3195..3713
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3344..3344
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3714..3717
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3718..3968
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3969..3972
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3975..3980)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4039..4058)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4076..4096)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4433..4481
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4530..5211
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5230..6015)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6016..6227)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6467..6467
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6468..7020
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgATAGA AAAGTTGGCT GCGGCCGCGA
     2581 AATAGACTGG CCTGTCTAGA ATAGACTGGC CTGTCTACAA TAGACTGGCC TGTCTACAAT
     2641 AGACTGGCCT GTCTAGTATA GACTGGCCTG TCTAAGATAG ACTGGCCTGT CTACGGGTCT
     2701 AAGCTAGCTA GGCCACCCAC AATcCCACTa tcctttcgca agacccttcc tcTATATAAg
     2761 gaagttcatt tcatttggag agAacacggg ggactctaga CACCATGGTG AGTAAAGGAG
     2821 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC
     2881 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA
     2941 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGCC AACCCTGGTC ACCACCCTGA
     3001 CCTACgtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag
     3061 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt
     3121 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat
     3181 ttgttgatgt gcagGGCGTG CAGTGCTTCT CCCGTTACCC TGATCATATG AAGCGGCACG
     3241 ACTTCTTCAA GAGCGCCATG CCTGAGGGAT ACGTGCAGGA GAGGACCATC TTCTTCAAGG
     3301 ACGACGGGAA CTACAAGACA CGTGCTGAAG TCAAGTTTGA GGGTGACACC CTCGTCAACA
     3361 GGATCGAGCT TAAGGGAATC GATTTCAAGG AGGACGGAAA CATCCTCGGC CACAAGTTGG
     3421 AATACAACTA CAACTCCCAC AACGTATACA TCATGGCCGA CAAGCAAAAG AACGGCATCA
     3481 AAGCCAACTT CAAGACCCGC CACAACATCG AGGACGGCGG CGTGCAACTC GCTGATCATT
     3541 ATCAACAAAA TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT
     3601 CCACACAATC TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGATCACATG GTCCTTCTTG
     3661 AGTTTGTAAC AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAA TAAtgtcaag
     3721 ccattctctc gcagatgatg ttcactttgt gttttacttc ctttatgcat tcacagcaat
     3781 aaaagaaaga aatctccatc gcttttggtt ttcttctctg tcttaagtta gtcgttttcg
     3841 tgtctaatct attacttatc attgtaatag actcttcttc tattgagatt tgaatataaa
     3901 ctaaaacaca ttccatttta ctgtgttctc aacattcaga atgcaaacgg actaaccgta
     3961 gtgactcggc ttgaGTCTTC gggctagagc ggactagttc tagagcggcc gccaccgcgg
     4021 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     4081 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4141 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4201 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4261 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4321 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4381 atacggttat ccacagaatc aggggataac gcaggaaaga acatgaaggc cttgacagga
     4441 tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa
     4501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4981 actagaagaa cagtatttgg tatctgTgct ctgctgaagc cagttacctt cggaagaaga
     5041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaattcttgt
     5221 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa caaattcttc
     5281 caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca agataagcct gcctagcttc
     5341 aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc
     5401 cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac
     5461 atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag
     5521 cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac
     5581 caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt
     5641 ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc
     5701 gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac
     5761 agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa
     5821 agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact
     5881 gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg
     5941 ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat
     6001 caccgcttcc ctcatgatgt ttaactcctg aattaagccg cgccgcgaag cggtgtCggc
     6061 ttgaatgaat tgttaggcgt catcctgtgc tcccgagaac cagtaccagt acatcgctgt
     6121 ttcgttcgag acttgaggtc tagttttata cgtgaacagg tcaatgccgc cgagagtaaa
     6181 gccacaTttt gcgtacaaat tgcaggcagg tacattgttc gtttgtgCCA TGATGATATA
     6241 TTTTTATCTT GTGCAATGTA ACATCAGAGA TTTTGAGACA CAACGTGGCT TTGTTGAATA
     6301 AATCGAACTT TTGCTGAGTT GAAGGATCAG ATCACGCATC TTCCCGACAA CGCAGACCGT
     6361 TCCGTGGCAA AGCAAAAGTT CAAAATCACC AACTGGTCCA CCTACAACAA AGCTCTCATC
     6421 AACCGTGGCT CCCTCACTTT CTGGCTGGAT GATGGGGCGA TTCAGGAGAT CCCCATCCAA
     6481 CAGCCCGCCG TCGAGCGGGC TTTTTTATCC CCGGAAGCCT GTGGATAGAG GGTAGTTATC
     6541 CACGTGAAAC CGCTAATGCC CCGCAAAGCC TTGATTCACG GGGCTTTCCG GCCCGCTCCA
     6601 AAAACTATCC ACGTGAAATC GCTAATCAGG GTACGTGAAA TCGCTAATCG GAGTACGTGA
     6661 AATCGCTAAT AAGGTCACGT GAAATCGCTA ATCAAAAAGG CACGTGAGAA CGCTAATAGC
     6721 CCTTTCAGAT CAACAGCTTG CAAACACCCC TCGCTCCGGC AAGTAGTTAC AGCAAGTAGT
     6781 ATGTTCAATT AGCTTTTCAA TTATGAATAT ATATATCAAT TATTGGTCGC CCTTGGCTTG
     6841 TGGACAATGC GCTACGCGCA CCGGCTCCGC CCGTGGACAA CCGCAAGCGG TTGCCCACCG
     6901 TCGAGCGCCT TTGCCCACAA CCCGGCGGCC GGCCGCAACA GATCGTTTTA TAAATTTTTT
     6961 TTTTTGAAAA AGAAAAAGCC CGAAAGGCGG CAACCTCTCG GGCTTCTGGA TTTCCGATCC
     7021 ccggaattag actct
//