LOCUS       pJAB2240_proG1090::PhlF 5523 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2129
                     /label="PhlF"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2130..2150
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2151..2153
                     /label="stop "
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2154..2157
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2161..2385
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2386..2389
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2392..2395
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2398..2403)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2462..2481)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2499..2519)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2856..2904
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2953..3634
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3653..4438)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4439..4650)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4891..4891
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4892..5444
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGGCCCGGAC CCCCAGCAGA AGCAGCATCG
     1561 GATCTCTGAG AAGCCCCCAC ACCCACAAGG CCATCCTGAC CAGCACCATC GAGATCCTGA
     1621 AAGAGTGCGG CTACAGCGGC CTGAGCATCG AGTCTGTGGC TAGAAGGGCT GGCGCCTCCA
     1681 AGCCCACCAT CTACCGGTGG TGGACAAACA AGGCCGCCCT GATCGCCGAG GTGTACGAGA
     1741 ACGAGAGCGA GCAGGTCCGA AAGTTCCCCG ACCTGGGCAG CTTCAAGGCC GATCTGGACT
     1801 TCCTGCTGCG GAACCTGTGG AAAGTGTGGC GGGAAACCAT CTGCGGCGAG GCCTTCAGAT
     1861 GCGTGATCGC TGAGGCCCAG CTGGACCCTG CCACACTGAC CCAGCTGAAG GACCAGTTCA
     1921 TGGAACGGCG GAGAGAGATG CCCAAGAAAC TGGTGGAAAA CGCCATCAGC AACGGCGAGC
     1981 TGCCCAAGGA CACCAACCGG GAACTGCTGC TGGATATGAT CTTCGGCTTC TGCTGGTACA
     2041 GACTGCTGAC CGAGCAGCTG ACCGTGGAAC AGGATATCGA GGAATTCACC TTTCTGCTGA
     2101 TCAACGGCGT GTGCCCCGGC ACCCAGAGAc cgaagaagaa gaggaaggtt TAGtgtcggt
     2161 acgctgaaat caccagtctc tctctacaaa tctatctctc tctattttct ccataaataa
     2221 tgtgtgagta gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt
     2281 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     2341 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcgatgcttg atactacGTC
     2401 TTCgggctag agcggactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     2461 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     2521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2641 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2701 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2821 atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg gcgggtaaac
     2881 taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag
     2941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3421 tggtatctgT gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc
     3481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3601 gaacgaaaac tcacgttaag ggattttggt catgaattct tgttagacat tatttgccga
     3661 ctaccttggt gatctcgcct ttcacgtagt gaacaaattc ttccaactga tctgcgcgcg
     3721 aggccaagcg atcttcttgt ccaagataag cctgcctagc ttcaagtatg acgggctgat
     3781 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     3841 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     3901 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     3961 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     4021 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     4081 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     4141 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     4201 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     4261 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
     4321 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     4381 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga
     4441 tgtttaactc ctgaattaag ccgcgccgcg aagcggtgtC ggcttgaatg aattgttagg
     4501 cgtcatcctg tgctcccgag aaccagtacc agtacatcgc tgtttcgttc gagacttgag
     4561 gtctagtttt atacgtgaac aggtcaatgc cgccgagagt aaagccacaT tttgcgtaca
     4621 aattgcaggc aggtacattg ttcgtttgtg CCATGATGAT ATATTTTTAT CTTGTGCAAT
     4681 GTAACATCAG AGATTTTGAG ACACAACGTG GCTTTGTTGA ATAAATCGAA CTTTTGCTGA
     4741 GTTGAAGGAT CAGATCACGC ATCTTCCCGA CAACGCAGAC CGTTCCGTGG CAAAGCAAAA
     4801 GTTCAAAATC ACCAACTGGT CCACCTACAA CAAAGCTCTC ATCAACCGTG GCTCCCTCAC
     4861 TTTCTGGCTG GATGATGGGG CGATTCAGGC AGATCCCCAT CCAACAGCCC GCCGTCGAGC
     4921 GGGCTTTTTT ATCCCCGGAA GCCTGTGGAT AGAGGGTAGT TATCCACGTG AAACCGCTAA
     4981 TGCCCCGCAA AGCCTTGATT CACGGGGCTT TCCGGCCCGC TCCAAAAACT ATCCACGTGA
     5041 AATCGCTAAT CAGGGTACGT GAAATCGCTA ATCGGAGTAC GTGAAATCGC TAATAAGGTC
     5101 ACGTGAAATC GCTAATCAAA AAGGCACGTG AGAACGCTAA TAGCCCTTTC AGATCAACAG
     5161 CTTGCAAACA CCCCTCGCTC CGGCAAGTAG TTACAGCAAG TAGTATGTTC AATTAGCTTT
     5221 TCAATTATGA ATATATATAT CAATTATTGG TCGCCCTTGG CTTGTGGACA ATGCGCTACG
     5281 CGCACCGGCT CCGCCCGTGG ACAACCGCAA GCGGTTGCCC ACCGTCGAGC GCCTTTGCCC
     5341 ACAACCCGGC GGCCGGCCGC AACAGATCGT TTTATAAATT TTTTTTTTTG AAAAAGAAAA
     5401 AGCCCGAAAG GCGGCAACCT CTCGGGCTTC TGGATTTCCG ATCCccggaa ttagactctg
     5461 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     5521 tgg
//