LOCUS       pJAB2282_proG1090::AmtR 5718 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein         1530..2195
                     /label="AmtR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2196..2204
                     /label="Gly-Gly-Gly linker"
                     /ApEinfo_revcolor=#5f76b8
                     /ApEinfo_fwdcolor=#5f76b8
     misc_feature    2205..2324
                     /label="ERF4 repressor domain (Ohta et al., 2001)"
                     /ApEinfo_revcolor=#ae4748
                     /ApEinfo_fwdcolor=#ae4748
     misc_feature    2325..2345
                     /label="SV40NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2346..2348
                     /label="stop"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2349..2352
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2356..2580
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2581..2584
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2587..2590
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2593..2598)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2657..2676)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2694..2714)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3051..3099
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3148..3829
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3848..4633)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4634..4845)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5086..5086
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5087..5639
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGGCTGGCGC CGTGGGCAGA CCCAGAAGAT
     1561 CTGCTCCTCG GAGAGCCGGC AAGAACCCCC GGGAAGAGAT TCTGGATGCC AGCGCCGAGC
     1621 TGTTCACCAG ACAGGGCTTT GCCACCACCA GCACCCACCA GATTGCCGAC GCTGTGGGCA
     1681 TCAGACAGGC CAGCCTGTAC TACCACTTCC CCAGCAAGAC CGAGATCTTC CTGACCCTGC
     1741 TGAAAAGCAC CGTGGAACCC TCCACCGTGC TGGCCGAGGA TCTGTCTACC CTGGACGCCG
     1801 GACCCGAAAT GAGACTGTGG GCTATCGTGG CCAGCGAAGT GCGGCTGCTG CTGAGCACCA
     1861 AGTGGAACGT GGGCCGGCTG TACCAGCTGC CCATCGTGGG CTCTGAGGAA TTCGCCGAGT
     1921 ACCACAGCCA GCGCGAGGCC CTGACCAACG TGTTCAGAGA TCTGGCCACC GAGATTGTGG
     1981 GCGACGACCC CAGAGCCGAA CTGCCCTTCC ACATCACCAT GAGCGTGATC GAGATGCGGC
     2041 GGAACGACGG CAAGATCCCT AGCCCTCTGA GCGCCGACTC TCTGCCCGAG ACAGCCATTA
     2101 TGCTGGCTGA CGCCTCCCTG GCTGTGCTGG GAGCACCTCT GCCTGCCGAC AGAGTGGAAA
     2161 AGACACTGGA ACTGATCAAG CAGGCCGACG CCAAGGGAGG AGGTGCTTGT GGTGCCCAAA
     2221 GCGACTCTGA TTCGTCATCG GTCGTTGATT TCGAAGGTGG GATGGAGAAG AGATCTCAGC
     2281 TGTTAGATCT AGATCTTAAT TTGCCTCCTC CATCGGAACA GGCCCCGAAG AAGAAGAGGA
     2341 AGGTTTAGtg tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat
     2401 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg
     2461 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     2521 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgat
     2581 gcttgatact acGTCTTCgg gctagagcgg actagttcta gagcggccgc caccgcggtg
     2641 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     2701 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2761 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2821 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2881 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2941 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3001 acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata
     3061 tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg
     3121 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3181 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3241 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3301 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3361 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3421 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3481 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3541 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3601 tagaagaaca gtatttggta tctgTgctct gctgaagcca gttaccttcg gaagaagagt
     3661 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3721 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3781 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga attcttgtta
     3841 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca
     3901 actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa
     3961 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     4021 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     4081 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     4141 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     4201 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     4261 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     4321 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     4381 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     4441 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     4501 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     4561 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     4621 ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtCggctt
     4681 gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt
     4741 cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc
     4801 cacaTtttgc gtacaaattg caggcaggta cattgttcgt ttgtgCCATG ATGATATATT
     4861 TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA ACGTGGCTTT GTTGAATAAA
     4921 TCGAACTTTT GCTGAGTTGA AGGATCAGAT CACGCATCTT CCCGACAACG CAGACCGTTC
     4981 CGTGGCAAAG CAAAAGTTCA AAATCACCAA CTGGTCCACC TACAACAAAG CTCTCATCAA
     5041 CCGTGGCTCC CTCACTTTCT GGCTGGATGA TGGGGCGATT CAGGCAGATC CCCATCCAAC
     5101 AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC
     5161 ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA
     5221 AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA
     5281 ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC
     5341 CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA
     5401 TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT
     5461 GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT
     5521 CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT AAATTTTTTT
     5581 TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT TTCCGATCCc
     5641 cggaattaga ctctggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     5701 gagctgttgg ctggctgg
//