LOCUS       pJAB2283_proG1090::AmtR 5760 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein         1530..2195
                     /label="AmtR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2196..2204
                     /label="Gly-Gly-Gly linker"
                     /ApEinfo_revcolor=#5f76b8
                     /ApEinfo_fwdcolor=#5f76b8
     misc_feature    2205..2366
                     /label="AtZAT10 repression domain"
                     /ApEinfo_revcolor=#af4a53
                     /ApEinfo_fwdcolor=#af4a53
     misc_feature    2367..2387
                     /label="SV40NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2388..2390
                     /label="AtERF4"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2391..2394
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2398..2622
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2623..2626
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2629..2632
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2635..2640)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2699..2718)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2736..2756)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3093..3141
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3190..3871
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3890..4675)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4676..4887)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5128..5128
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5129..5681
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGGCTGGCGC CGTGGGCAGA CCCAGAAGAT
     1561 CTGCTCCTCG GAGAGCCGGC AAGAACCCCC GGGAAGAGAT TCTGGATGCC AGCGCCGAGC
     1621 TGTTCACCAG ACAGGGCTTT GCCACCACCA GCACCCACCA GATTGCCGAC GCTGTGGGCA
     1681 TCAGACAGGC CAGCCTGTAC TACCACTTCC CCAGCAAGAC CGAGATCTTC CTGACCCTGC
     1741 TGAAAAGCAC CGTGGAACCC TCCACCGTGC TGGCCGAGGA TCTGTCTACC CTGGACGCCG
     1801 GACCCGAAAT GAGACTGTGG GCTATCGTGG CCAGCGAAGT GCGGCTGCTG CTGAGCACCA
     1861 AGTGGAACGT GGGCCGGCTG TACCAGCTGC CCATCGTGGG CTCTGAGGAA TTCGCCGAGT
     1921 ACCACAGCCA GCGCGAGGCC CTGACCAACG TGTTCAGAGA TCTGGCCACC GAGATTGTGG
     1981 GCGACGACCC CAGAGCCGAA CTGCCCTTCC ACATCACCAT GAGCGTGATC GAGATGCGGC
     2041 GGAACGACGG CAAGATCCCT AGCCCTCTGA GCGCCGACTC TCTGCCCGAG ACAGCCATTA
     2101 TGCTGGCTGA CGCCTCCCTG GCTGTGCTGG GAGCACCTCT GCCTGCCGAC AGAGTGGAAA
     2161 AGACACTGGA ACTGATCAAG CAGGCCGACG CCAAGGGAGG AGGTGAAGGT GCGGGGTCCA
     2221 CTAGCCACGT TAGCAGTAGC CACCGTGGGT TTGACCTCAA CATCCCTCCG ATCCCTGAAT
     2281 TCTCGATGGT CAACGGAGAC GACGAAGTCA TGAGCCCTAT GCCGGCGAAG AAGCCTCGGT
     2341 TTGACTTTCC GGTCAAACTT CAACTTCCGA AGAAGAAGAG GAAGGTTTAG tgtcggtacg
     2401 ctgaaatcac cagtctctct ctacaaatct atctctctct attttctcca taaataatgt
     2461 gtgagtagtt tcccgataag ggaaattagg gttcttatag ggtttcgctc atgtgttgag
     2521 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2581 taattcctaa aaccaaaatc cagtactaaa atccagatcg atgcttgata ctacGTCTTC
     2641 gggctagagc ggactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
     2701 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     2761 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2821 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2881 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2941 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3001 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3061 aggggataac gcaggaaaga acatgaaggc cttgacagga tatattggcg ggtaaactaa
     3121 gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa
     3181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3661 tatctgTgct ctgctgaagc cagttacctt cggaagaaga gttggtagct cttgatccgg
     3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3841 cgaaaactca cgttaaggga ttttggtcat gaattcttgt tagacattat ttgccgacta
     3901 ccttggtgat ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg
     3961 ccaagcgatc ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact
     4021 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     4081 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     4141 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     4201 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     4261 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     4321 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     4381 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     4441 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     4501 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     4561 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     4621 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt
     4681 ttaactcctg aattaagccg cgccgcgaag cggtgtCggc ttgaatgaat tgttaggcgt
     4741 catcctgtgc tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc
     4801 tagttttata cgtgaacagg tcaatgccgc cgagagtaaa gccacaTttt gcgtacaaat
     4861 tgcaggcagg tacattgttc gtttgtgCCA TGATGATATA TTTTTATCTT GTGCAATGTA
     4921 ACATCAGAGA TTTTGAGACA CAACGTGGCT TTGTTGAATA AATCGAACTT TTGCTGAGTT
     4981 GAAGGATCAG ATCACGCATC TTCCCGACAA CGCAGACCGT TCCGTGGCAA AGCAAAAGTT
     5041 CAAAATCACC AACTGGTCCA CCTACAACAA AGCTCTCATC AACCGTGGCT CCCTCACTTT
     5101 CTGGCTGGAT GATGGGGCGA TTCAGGCAGA TCCCCATCCA ACAGCCCGCC GTCGAGCGGG
     5161 CTTTTTTATC CCCGGAAGCC TGTGGATAGA GGGTAGTTAT CCACGTGAAA CCGCTAATGC
     5221 CCCGCAAAGC CTTGATTCAC GGGGCTTTCC GGCCCGCTCC AAAAACTATC CACGTGAAAT
     5281 CGCTAATCAG GGTACGTGAA ATCGCTAATC GGAGTACGTG AAATCGCTAA TAAGGTCACG
     5341 TGAAATCGCT AATCAAAAAG GCACGTGAGA ACGCTAATAG CCCTTTCAGA TCAACAGCTT
     5401 GCAAACACCC CTCGCTCCGG CAAGTAGTTA CAGCAAGTAG TATGTTCAAT TAGCTTTTCA
     5461 ATTATGAATA TATATATCAA TTATTGGTCG CCCTTGGCTT GTGGACAATG CGCTACGCGC
     5521 ACCGGCTCCG CCCGTGGACA ACCGCAAGCG GTTGCCCACC GTCGAGCGCC TTTGCCCACA
     5581 ACCCGGCGGC CGGCCGCAAC AGATCGTTTT ATAAATTTTT TTTTTTGAAA AAGAAAAAGC
     5641 CCGAAAGGCG GCAACCTCTC GGGCTTCTGG ATTTCCGATC Cccggaatta gactctggct
     5701 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//