LOCUS       pJAB2296_proUBQ10::PIP2 7704 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2556..3409
                     /label="35S promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2629..2629
                     /label="G->C mutation to remove Bsa1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3284..3311
                     /label="sJAB064"
                     /note="sequence: gcaagtggattgatgtgatatctccact"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3310..3330
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    3319..3319
                     /label="G deleted"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3328..3330
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3331..3333
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3362..3368
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    3392..3392
                     /label="+1 P35S"
                     /ApEinfo_revcolor=#00c500
                     /ApEinfo_fwdcolor=#00c500
     misc_feature    3410..3410
                     /label="A->T mutation to remove ATG in PhlFO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3411..3439
                     /label="PhlFO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    3440..3443
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3444..3644
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3645..3833
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3834..4382
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3983..3983
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4350..4379
                     /label="NLS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4383..4386
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4387..4637
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4638..4641
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4644..4649)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4708..4727)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4745..4765)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5102..5150
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5199..5880
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5899..6684)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6685..6896)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7136..7136
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7137..7689
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgGGTCC CCAGATTTGC CTTTTCAATT
     2581 TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGCT CTCATCAAGA
     2641 CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG
     2701 CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA
     2761 TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA
     2821 TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA
     2881 TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC
     2941 TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG
     3001 TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC
     3061 AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA
     3121 TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA
     3181 AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA
     3241 GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG
     3301 ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT
     3361 CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCTAGAT TGATACGAAA
     3421 CGTACCGTAT CGTTAAGGTc accATGGTGA GTAAAGGAGA AGAACTTTTC ACTGGAGTTG
     3481 TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT GTCAGTGGAG
     3541 AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC ACTACTGGAA
     3601 AACTACCTGT TCCATGGCCA ACCCTGGTCA CCACCCTGAC CTACgtaagt ttctgcttct
     3661 acctttgata tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt
     3721 tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt
     3781 atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg cagGGCGTGC
     3841 AGTGCTTCTC CCGTTACCCT GATCATATGA AGCGGCACGA CTTCTTCAAG AGCGCCATGC
     3901 CTGAGGGATA CGTGCAGGAG AGGACCATCT TCTTCAAGGA CGACGGGAAC TACAAGACAC
     3961 GTGCTGAAGT CAAGTTTGAG GGTGACACCC TCGTCAACAG GATCGAGCTT AAGGGAATCG
     4021 ATTTCAAGGA GGACGGAAAC ATCCTCGGCC ACAAGTTGGA ATACAACTAC AACTCCCACA
     4081 ACGTATACAT CATGGCCGAC AAGCAAAAGA ACGGCATCAA AGCCAACTTC AAGACCCGCC
     4141 ACAACATCGA GGACGGCGGC GTGCAACTCG CTGATCATTA TCAACAAAAT ACTCCAATTG
     4201 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA
     4261 AAGATCCCAA CGAAAAGAGA GATCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA
     4321 TTACACATGG CATGGATGAA CTATACAAAC CTAAGAAAAA GCGTAAGGTC GAGGACCCTT
     4381 AAtgtcaagc cattctctcg cagatgatgt tcactttgtg ttttacttcc tttatgcatt
     4441 cacagcaata aaagaaagaa atctccatcg cttttggttt tcttctctgt cttaagttag
     4501 tcgttttcgt gtctaatcta ttacttatca ttgtaataga ctcttcttct attgagattt
     4561 gaatataaac taaaacacat tccattttac tgtgttctca acattcagaa tgcaaacgga
     4621 ctaaccgtag tgactcggct tgaGTCTTCg ggctagagcg gactagttct agagcggccg
     4681 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4741 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4801 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4861 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4921 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4981 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5041 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgaaggcc
     5101 ttgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg
     5161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5641 tacggctaca ctagaagaac agtatttggt atctgTgctc tgctgaagcc agttaccttc
     5701 ggaagaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5881 aattcttgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac
     5941 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg
     6001 cctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     6061 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     6121 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     6181 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     6241 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     6301 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     6361 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     6421 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     6481 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
     6541 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     6601 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
     6661 ttcggcgatc accgcttccc tcatgatgtt taactcctga attaagccgc gccgcgaagc
     6721 ggtgtCggct tgaatgaatt gttaggcgtc atcctgtgct cccgagaacc agtaccagta
     6781 catcgctgtt tcgttcgaga cttgaggtct agttttatac gtgaacaggt caatgccgcc
     6841 gagagtaaag ccacaTtttg cgtacaaatt gcaggcaggt acattgttcg tttgtgCCAT
     6901 GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC AACGTGGCTT
     6961 TGTTGAATAA ATCGAACTTT TGCTGAGTTG AAGGATCAGA TCACGCATCT TCCCGACAAC
     7021 GCAGACCGTT CCGTGGCAAA GCAAAAGTTC AAAATCACCA ACTGGTCCAC CTACAACAAA
     7081 GCTCTCATCA ACCGTGGCTC CCTCACTTTC TGGCTGGATG ATGGGGCGAT TCAGGAGATC
     7141 CCCATCCAAC AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG
     7201 GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG
     7261 CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG
     7321 AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC
     7381 GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA
     7441 GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC
     7501 CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT
     7561 TGCCCACCGT CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT
     7621 AAATTTTTTT TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT
     7681 TTCCGATCCc cggaattaga ctct
//