LOCUS       pJAB2462_proUBQ10::PIP2 7702 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2556..3409
                     /label="35S promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2629..2629
                     /label="G->C mutation to remove Bsa1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3310..3330
                     /label="as-1 (Lam et al, 1989)"
                     /ApEinfo_revcolor=#213e1f
                     /ApEinfo_fwdcolor=#213e1f
     misc_feature    3319..3319
                     /label="G deleted"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3328..3330
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3331..3333
                     /label="CACAAT box P35S"
                     /ApEinfo_revcolor=#00ae00
                     /ApEinfo_fwdcolor=#00ae00
     misc_feature    3362..3368
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    3392..3392
                     /label="+1 P35S"
                     /ApEinfo_revcolor=#00c500
                     /ApEinfo_fwdcolor=#00c500
     misc_feature    3410..3410
                     /label="A->T mutation to remove ATG in PhlFO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3414..3437
                     /label="LmrAO"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    3438..3441
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3442..3642
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3643..3831
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3832..4380
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3981..3981
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4348..4377
                     /label="NLS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4381..4384
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4385..4635
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4636..4639
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4642..4647)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4706..4725)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4743..4763)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5100..5148
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5197..5878
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5897..6682)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6683..6894)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7134..7134
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7135..7687
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgGGTCC CCAGATTTGC CTTTTCAATT
     2581 TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGCT CTCATCAAGA
     2641 CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG
     2701 CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA
     2761 TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA
     2821 TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA
     2881 TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC
     2941 TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG
     3001 TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC
     3061 AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA
     3121 TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA
     3181 AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA
     3241 GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG
     3301 ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT
     3361 CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACTCTAGAT TGAGATAATA
     3421 GACCAGTCAC TATATTTcac cATGGTGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC
     3481 CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG
     3541 GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA
     3601 CTACCTGTTC CATGGCCAAC CCTGGTCACC ACCCTGACCT ACgtaagttt ctgcttctac
     3661 ctttgatata tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt
     3721 tttcaaaata aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat
     3781 attttaattt ataacttttc taatatatga ccaaaatttg ttgatgtgca gGGCGTGCAG
     3841 TGCTTCTCCC GTTACCCTGA TCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCT
     3901 GAGGGATACG TGCAGGAGAG GACCATCTTC TTCAAGGACG ACGGGAACTA CAAGACACGT
     3961 GCTGAAGTCA AGTTTGAGGG TGACACCCTC GTCAACAGGA TCGAGCTTAA GGGAATCGAT
     4021 TTCAAGGAGG ACGGAAACAT CCTCGGCCAC AAGTTGGAAT ACAACTACAA CTCCCACAAC
     4081 GTATACATCA TGGCCGACAA GCAAAAGAAC GGCATCAAAG CCAACTTCAA GACCCGCCAC
     4141 AACATCGAGG ACGGCGGCGT GCAACTCGCT GATCATTATC AACAAAATAC TCCAATTGGC
     4201 GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA
     4261 GATCCCAACG AAAAGAGAGA TCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT
     4321 ACACATGGCA TGGATGAACT ATACAAACCT AAGAAAAAGC GTAAGGTCGA GGACCCTTAA
     4381 tgtcaagcca ttctctcgca gatgatgttc actttgtgtt ttacttcctt tatgcattca
     4441 cagcaataaa agaaagaaat ctccatcgct tttggttttc ttctctgtct taagttagtc
     4501 gttttcgtgt ctaatctatt acttatcatt gtaatagact cttcttctat tgagatttga
     4561 atataaacta aaacacattc cattttactg tgttctcaac attcagaatg caaacggact
     4621 aaccgtagtg actcggcttg aGTCTTCggg ctagagcgga ctagttctag agcggccgcc
     4681 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     4741 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4801 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4861 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4921 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4981 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5041 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgaaggcctt
     5101 gacaggatat attggcgggt aaactaagtc gctgtatgtg tttgtttgag atctcatgtg
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5641 cggctacact agaagaacag tatttggtat ctgTgctctg ctgaagccag ttaccttcgg
     5701 aagaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa
     5881 ttcttgttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtgaacaa
     5941 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga taagcctgcc
     6001 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     6061 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     6121 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     6181 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     6241 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     6301 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
     6361 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     6421 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     6481 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     6541 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     6601 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
     6661 cggcgatcac cgcttccctc atgatgttta actcctgaat taagccgcgc cgcgaagcgg
     6721 tgtCggcttg aatgaattgt taggcgtcat cctgtgctcc cgagaaccag taccagtaca
     6781 tcgctgtttc gttcgagact tgaggtctag ttttatacgt gaacaggtca atgccgccga
     6841 gagtaaagcc acaTtttgcg tacaaattgc aggcaggtac attgttcgtt tgtgCCATGA
     6901 TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA CGTGGCTTTG
     6961 TTGAATAAAT CGAACTTTTG CTGAGTTGAA GGATCAGATC ACGCATCTTC CCGACAACGC
     7021 AGACCGTTCC GTGGCAAAGC AAAAGTTCAA AATCACCAAC TGGTCCACCT ACAACAAAGC
     7081 TCTCATCAAC CGTGGCTCCC TCACTTTCTG GCTGGATGAT GGGGCGATTC AGGAGATCCC
     7141 CATCCAACAG CCCGCCGTCG AGCGGGCTTT TTTATCCCCG GAAGCCTGTG GATAGAGGGT
     7201 AGTTATCCAC GTGAAACCGC TAATGCCCCG CAAAGCCTTG ATTCACGGGG CTTTCCGGCC
     7261 CGCTCCAAAA ACTATCCACG TGAAATCGCT AATCAGGGTA CGTGAAATCG CTAATCGGAG
     7321 TACGTGAAAT CGCTAATAAG GTCACGTGAA ATCGCTAATC AAAAAGGCAC GTGAGAACGC
     7381 TAATAGCCCT TTCAGATCAA CAGCTTGCAA ACACCCCTCG CTCCGGCAAG TAGTTACAGC
     7441 AAGTAGTATG TTCAATTAGC TTTTCAATTA TGAATATATA TATCAATTAT TGGTCGCCCT
     7501 TGGCTTGTGG ACAATGCGCT ACGCGCACCG GCTCCGCCCG TGGACAACCG CAAGCGGTTG
     7561 CCCACCGTCG AGCGCCTTTG CCCACAACCC GGCGGCCGGC CGCAACAGAT CGTTTTATAA
     7621 ATTTTTTTTT TTGAAAAAGA AAAAGCCCGA AAGGCGGCAA CCTCTCGGGC TTCTGGATTT
     7681 CCGATCCccg gaattagact ct
//