LOCUS       pJAB2488_proG1090::IcaR 5478 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label="T-Border (left)"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      complement(76..359)
                     /label="NOS\terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_marker     complement(367..918)
                     /label="BAR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(640..640)
                     /label="G->T mutation to remove Bbs1 site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(935..1227)
                     /label="pNOS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1265..1270
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1273..1276
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1279..1282
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1283..1525
                     /label="G10-90 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1526..1529
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1530..2084
                     /label="IcaR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2085..2105
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2106..2108
                     /label="Activator stop codon"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2109..2112
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     terminator      2116..2340
                     /label="19STer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2341..2344
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2347..2350
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2353..2358)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(2417..2436)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(2454..2474)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2811..2859
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2908..3589
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(3608..4393)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4394..4605)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4846..4846
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4847..5399
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtaatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggaC acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
      961 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1021 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1081 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     1141 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1201 ccttaattct ccgctcatga tcgataacat gtcgactatc ggacgagctc aaagtttaac
     1261 gTgtGAAGAC gtgcttctaa tgtagtttaa actgaaggcg ggaaacgaca atctgatcca
     1321 agctcaagct aagcttgcat gcctgcagga tatcgtggat ccaagcttgc cacgtgccgc
     1381 cacgtgccgc cacgtgccgc cacgtgcctc tagaggatcc atctccactg acgtaaggga
     1441 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1501 tttggagagg acacgctggg atcCccaccA TGAAGGACAA GATCATCGAC AACGCCATCA
     1561 CCCTGTTCAG CGAGAAGGGC TACGACGGCA CCACACTGGA CGACATCAGC AAGAGCGTGA
     1621 ACATCAAGAA GGCCAGCCTG TACTACCACT ACGACAACAA AGAGGAAATC TACCGGAAGT
     1681 CCGTCGAGAA CTGCTTCAAC TACTTCATCG ACTTTCTGCT GCGGAACCAC GACGATAACT
     1741 ACAGCATCGA CGGCCTGTAC CAGTTCCTGT TCAAGTTTAT CTTCGACGTG GACGAGCGGT
     1801 ACATCAAGCT GTACGTGCAG CTGAGCAGCG CCCCTGAGGC CCTGAACAGC GAGATCAAGC
     1861 ACCATCTGCA GGAAATCAAC ACCACCCTGC ACGACGAGCT GATCAAGTAC TACGACCCCA
     1921 CCCACATTGC TCTGAACAAA GAAGATTTCA TCAACCTGAT CCTGCTGTTC CTGGAAACCT
     1981 GGTACTTCCG GGCCAGCTTC TCCCAGAAAT TCGGCGTGAT CGAGGACAGC AAGAACCGGT
     2041 TCAAGGACCA GGTGTACAGC CTGCTGAACG TGTTCCTGAA GAAGccgaag aagaagagga
     2101 aggtttagtg tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat
     2161 tttctccata aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg
     2221 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     2281 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgat
     2341 gcttgatact acGTCTTCgg gctagagcgg actagttcta gagcggccgc caccgcggtg
     2401 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     2461 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2521 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2581 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata
     2821 tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg
     2881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3361 tagaagaaca gtatttggta tctgTgctct gctgaagcca gttaccttcg gaagaagagt
     3421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga attcttgtta
     3601 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca
     3661 actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa
     3721 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     3781 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     3841 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     3901 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     3961 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     4021 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     4081 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     4141 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     4201 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     4261 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     4321 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     4381 ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtCggctt
     4441 gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt
     4501 cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc
     4561 cacaTtttgc gtacaaattg caggcaggta cattgttcgt ttgtgCCATG ATGATATATT
     4621 TTTATCTTGT GCAATGTAAC ATCAGAGATT TTGAGACACA ACGTGGCTTT GTTGAATAAA
     4681 TCGAACTTTT GCTGAGTTGA AGGATCAGAT CACGCATCTT CCCGACAACG CAGACCGTTC
     4741 CGTGGCAAAG CAAAAGTTCA AAATCACCAA CTGGTCCACC TACAACAAAG CTCTCATCAA
     4801 CCGTGGCTCC CTCACTTTCT GGCTGGATGA TGGGGCGATT CAGGCAGATC CCCATCCAAC
     4861 AGCCCGCCGT CGAGCGGGCT TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC
     4921 ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA
     4981 AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA
     5041 ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC
     5101 CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA
     5161 TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT
     5221 GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT
     5281 CGAGCGCCTT TGCCCACAAC CCGGCGGCCG GCCGCAACAG ATCGTTTTAT AAATTTTTTT
     5341 TTTTGAAAAA GAAAAAGCCC GAAAGGCGGC AACCTCTCGG GCTTCTGGAT TTCCGATCCc
     5401 cggaattaga ctctggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     5461 gagctgttgg ctggctgg
//