LOCUS       pJAB2493_proUBQ10::PIP2 7059 bp ds-DNA     circular     30-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /label="LB left border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    34..674
                     /label="region used as proUBQ10 promoter (JANB)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    369..672
                     /label="UBQ10 5' intron"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    675..678
                     /label="scar"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             679..1540
                     /label="PIP2A"
                     /ApEinfo_revcolor=#104aff
                     /ApEinfo_fwdcolor=#104aff
     CDS             1546..2256
                     /label="mCherry"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1974..1974
                     /label="G->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2280..2529
                     /label="nos\3'"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2544..2549
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2552..2555
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2582..2602
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2605..2625
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2628..2648
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2651..2671
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2674..2694
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2697..2717
                     /label="lacO1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2725..2725
                     /label="C ->A mutation to remove Bsa1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2738..2741
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2742..2747
                     /label="CACAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2742..2824
                     /label="min35S promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2749..2753
                     /label="CCAAT box"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     primer          2750..2774
                     /label="sJAB062"
                     /note="sequence: cactatcctttcgcaagacccttcc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2777..2783
                     /label="TATA extended"
                     /ApEinfo_revcolor=#00bb00
                     /ApEinfo_fwdcolor=#00bb00
     misc_feature    2807..2807
                     /label="TSS should be a 'G'"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    2825..2828
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2829..3029
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3030..3218
                     /label="ST-LS1 intron2"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    3219..3737
                     /label="mGFP6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3368..3368
                     /label="A->T mutation to remove Bbs1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3738..3741
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3742..3992
                     /label="ADH terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3993..3996
                     /label="Bsa1 seam"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3999..4004)
                     /label="Bbs1"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     primer_bind     complement(4063..4082)
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(4100..4120)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4457..4505
                     /label="RB right border"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4554..5235
                     /label="colE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_marker     complement(5254..6039)
                     /label="Spec-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(6040..6251)
                     /label="spec promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6491..6491
                     /label="C->A mutation"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6492..7044
                     /label="pSa ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tggcaggata tattgtggtg taacgttAAG CTTTGGTTGT CGACGAGTCA GTAATAAACG
       61 GCGTCAAAGT GGTTGCAGCC GGCACACACG AGTCGTGTTT ATCAACTCAA AGCACAAATA
      121 CTTTTCCTCA ACCTAAAAAT AAGGCAATTA GCCAAAAACA ACTTTGCGTG TAAACAACGC
      181 TCAATACACG TGTCATTTTA TTATTAGCTA TTGCTTCACC GCCTTAGCTT TCTCGTGACC
      241 TAGTCGTCCT CGTCTTTTCT TCTTCTTCTT CTATAAAACA ATACCCAAAG AGCTCTTCTT
      301 CTTCACAATT CAGATTTCAA TTTCTCAAAA TCTTAAAAAC TTTCTCTCAA TTCTCTCTAC
      361 CGTGATCAAG GTAAATTTCT GTGTTCCTTA TTCTCTCAAA ATCTTCGATT TTGTTTTCGT
      421 TCGATCCCAA TTTCGTATAT GTTCTTTGGT TTAGATTCTG TTAATCTTAG ATCGTAGACG
      481 ATTTTCTGGG TTTGATCGTT AGATATCATC TTAATTCTCG ATTAGGGTTT CATAGATATC
      541 ATCCGATTTG TTCAAATAAT TTGAGTTTTG TCGAATAATT ACTCTTCGAT TTGTGATTTC
      601 TATCTAGATC TGGTGTTAGT TTCTAGTTTG TGCGATCGAA TTTGTCGATT AATCTGAGTT
      661 TTTCTGATTA ACAGCACCat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcAgag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggCgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtCt agccgcagag
     1201 atcattggta ctttcgttct tgtctacacC gtAttctctg cCactgaccc caaacgtagt
     1261 gcCagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctAag agcttcaggt
     1501 tctaagtctc tTggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaaTAAGACc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga Ggagctgtac AAGtaaagcg gccgcccggc tgcaGATCgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagGAAGACg taatgATAGA AAAGTTGGCT GCGGCCGCGA
     2581 AAATTGTGAG CGGATAACAA TTgaAATTGT GAGCGGATAA CAATTGGAAT TGTGAGCGGA
     2641 TAACAATTCA AATTGTGAGC GGATAACAAT TGTAATTGTG AGCGGATAAC AATTAGAATT
     2701 GTGAGCGGAT AACAATTCGG GTCTAAGCTA GCTAGGCCAC CCACAATcCC ACTatccttt
     2761 cgcaagaccc ttcctcTATA TAAggaagtt catttcattt ggagagAaca cgggggactc
     2821 tagaCACCAT GGTGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG
     2881 AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG
     2941 CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT
     3001 GGCCAACCCT GGTCACCACC CTGACCTACg taagtttctg cttctacctt tgatatatat
     3061 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa
     3121 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata
     3181 acttttctaa tatatgacca aaatttgttg atgtgcagGG CGTGCAGTGC TTCTCCCGTT
     3241 ACCCTGATCA TATGAAGCGG CACGACTTCT TCAAGAGCGC CATGCCTGAG GGATACGTGC
     3301 AGGAGAGGAC CATCTTCTTC AAGGACGACG GGAACTACAA GACACGTGCT GAAGTCAAGT
     3361 TTGAGGGTGA CACCCTCGTC AACAGGATCG AGCTTAAGGG AATCGATTTC AAGGAGGACG
     3421 GAAACATCCT CGGCCACAAG TTGGAATACA ACTACAACTC CCACAACGTA TACATCATGG
     3481 CCGACAAGCA AAAGAACGGC ATCAAAGCCA ACTTCAAGAC CCGCCACAAC ATCGAGGACG
     3541 GCGGCGTGCA ACTCGCTGAT CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC
     3601 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA
     3661 AGAGAGATCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG
     3721 ATGAACTATA CAAATAAtgt caagccattc tctcgcagat gatgttcact ttgtgtttta
     3781 cttcctttat gcattcacag caataaaaga aagaaatctc catcgctttt ggttttcttc
     3841 tctgtcttaa gttagtcgtt ttcgtgtcta atctattact tatcattgta atagactctt
     3901 cttctattga gatttgaata taaactaaaa cacattccat tttactgtgt tctcaacatt
     3961 cagaatgcaa acggactaac cgtagtgact cggcttgaGT CTTCgggcta gagcggacta
     4021 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     4081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4441 aagaacatga aggccttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt
     4501 gtttgagatc tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg Tgctctgctg
     5041 aagccagtta ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5221 gggattttgg tcatgaattc ttgttagaca ttatttgccg actaccttgg tgatctcgcc
     5281 tttcacgtag tgaacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttg
     5341 tccaagataa gcctgcctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     5401 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     5461 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     5521 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     5581 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     5641 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     5701 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     5761 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     5821 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     5881 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     5941 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     6001 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact cctgaattaa
     6061 gccgcgccgc gaagcggtgt Cggcttgaat gaattgttag gcgtcatcct gtgctcccga
     6121 gaaccagtac cagtacatcg ctgtttcgtt cgagacttga ggtctagttt tatacgtgaa
     6181 caggtcaatg ccgccgagag taaagccaca Ttttgcgtac aaattgcagg caggtacatt
     6241 gttcgtttgt gCCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA
     6301 GACACAACGT GGCTTTGTTG AATAAATCGA ACTTTTGCTG AGTTGAAGGA TCAGATCACG
     6361 CATCTTCCCG ACAACGCAGA CCGTTCCGTG GCAAAGCAAA AGTTCAAAAT CACCAACTGG
     6421 TCCACCTACA ACAAAGCTCT CATCAACCGT GGCTCCCTCA CTTTCTGGCT GGATGATGGG
     6481 GCGATTCAGG AGATCCCCAT CCAACAGCCC GCCGTCGAGC GGGCTTTTTT ATCCCCGGAA
     6541 GCCTGTGGAT AGAGGGTAGT TATCCACGTG AAACCGCTAA TGCCCCGCAA AGCCTTGATT
     6601 CACGGGGCTT TCCGGCCCGC TCCAAAAACT ATCCACGTGA AATCGCTAAT CAGGGTACGT
     6661 GAAATCGCTA ATCGGAGTAC GTGAAATCGC TAATAAGGTC ACGTGAAATC GCTAATCAAA
     6721 AAGGCACGTG AGAACGCTAA TAGCCCTTTC AGATCAACAG CTTGCAAACA CCCCTCGCTC
     6781 CGGCAAGTAG TTACAGCAAG TAGTATGTTC AATTAGCTTT TCAATTATGA ATATATATAT
     6841 CAATTATTGG TCGCCCTTGG CTTGTGGACA ATGCGCTACG CGCACCGGCT CCGCCCGTGG
     6901 ACAACCGCAA GCGGTTGCCC ACCGTCGAGC GCCTTTGCCC ACAACCCGGC GGCCGGCCGC
     6961 AACAGATCGT TTTATAAATT TTTTTTTTTG AAAAAGAAAA AGCCCGAAAG GCGGCAACCT
     7021 CTCGGGCTTC TGGATTTCCG ATCCccggaa ttagactct
//